Recombinant Human Cytochrome c

Recombinant Human Cytochrome c/CYCS  (CF80)

 

产品说明(Description)

Recombinant Human Cytochrome C is produced by our E.coli expression system and the target gene encoding Gly2-Glu105 is expressed with a 6His tag at the C-terminus. 

Accession #: P99999

Known as: Cytochrome C; CYCS; CYC

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris HCl, 200mM NaCl, Ph8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.      

 

背景（Background）

Cytochrome C (CYCS) is a small heme protein that belongs to the cytochrome c family. It is found loosely associated with the inner membrane of the mitochondrion. Cytochrome C is a highly soluble protein that functions as a central component of the electron transport chain in mitochondria. CYCS transfers electrons between Complexes III (Coenzyme Q - Cyt C reductase) and IV (Cyt C oxidase). CYCS plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of Cytochrome C to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

 

电泳(SDS-PAGE)

 

参考文献（References）

Combining Whispering‐Gallery Mode Optical Biosensors with Microfluidics for Real‐Time Detection of Protein Secretion from Living Cells in Complex Media[J]. Ying‐Jen Chen. et al. Advanced Science. 2018.

 

FOR RESEARCH USE ONLY