Recombinant Human B3GAT3/β-1,3

Recombinant Human B3GAT3/β-1,3-Glucuronyltransferase 3  (CK05)

 

产品说明(Description)

Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 is produced by our E.coli expression system and the target gene encoding Glu72-Val335 is expressed with a 6His tag at the C-terminus. 

Accession #: O94766

Known as: B3GAT3;Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3;Beta-1;3-glucuronyltransferase 3;Glucuronosyltransferase I;GlcAT-I;GlcUAT-I;Gal beta-1;3-Gal-R glucuronyltransferase;;

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaC,2mM EDTA,20% Glycerol,pH 8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family. B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins , hows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY