Recombinant Human Vinculin

Recombinant Human Vinculin (C264)

产品说明(Description)

Recombinant Human Vinculin is produced by our E.coli expression system and the target gene encoding Pro2-Gln1066 is expressed. 

Accession #: AAH39174.1

Known as: Vinculin; Metavinculin; VCL

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

 

 

Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:	Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/α-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Circulating Anti-cytolethal Distending Toxin B and Anti-vinculin Antibodies as Biomarkers in Community and Healthcare Populations With Functional Dyspepsia and Irritable Bowel Syndrome[J].Talley NJ. et al. Clinical and Translational Gastroenterology. 2019.

Association between interstitial cells of Cajal and anti-vinculin antibody in human stomach[J]. Ji Hyun Kim. et al. Korean Journal of Physiology & Pharmacology. 2020.

Development and Validation of a Biomarker for Diarrhea-Predominant Irritable Bowel Syndrome in Human Subjects. Pimentel M. et al. PLoS ONE. 2015.

Methods for Detecting Post-Infectious Irritable Bowel Syndrome[J]. Pimentel. et al. 2020.

 

 

FOR RESEARCH USE ONLY