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Anti-GRP-78, clone C107
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文献和实验Single Clone Excision Protocol
500ml 20% maltose and 500ml 1 M MgSO4 to 50 ml LB broth). Next day: 3.If you're making new cells,spin them down 5 min at 1500 x g in the C0650 rotor in the Beckman centrifuge,then resuspend in about 40 ml 10 mM MgSO4,and quantify absorbance
Clone Genes From a Phage Library
from the desired phage Some preparatory items The library (one that I made from C. albicans genomic DNA) is kept at -80 degrees C in 0.5 ml aliquots. This solution is also stable in the refrigerator for several months at least. The library
Construction and Manipulation of Large-Insert Bacterial Clone Libraries
Introduction Construction of Large-Insert Bacterial Clone Libraries Fig. 1. A general procedure
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