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Anti-Tom20/Tomm20, clone 2F8.1
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文献和实验Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
A5754 anti-DNA-POD (clone MCA-33) Boehringer Mannhein 1544675 biotinylated dUTP Boehringer Mannhein 1093070 DAB tablets (100 tablets)
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