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- 默克密理博
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- 2026年01月30日
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Hyaluronidase, Bovine Testes 1PC x 25KU
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文献和实验TUNEL PROCEDURE IN BOVINE EMBRYOS
Hoescht 33342: Prepare Stock 1 by dissolving 25 mg Hoechst 33342 (Sigma B2261) in 2.5 ml of distilled water (10 mg/ml). Store at 4 C. On the day of use, prepare Stock 2 by diluting 5 µl Stock 1 in 10 ml PBS containing 1 mg/ml
Procedures for In Vitro Production of Bovine Embryos
with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap
TUNEL PROCEDURE IN BOVINE EMBRYOS
Prepare Stock 1 by dissolving 25 mg Hoechst 33342 (Sigma B2261) in 2.5 ml of distilled water (10 mg/ml). Store at 4 C. On the day of use, prepare Stock 2 by diluting 5 µl Stock 1 in 10 ml PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) to produce
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