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Caspase 10 Antibody

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  • ¥1200
  • affinity
  • AF0122
  • 美国
  • 2026年01月29日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 克隆性

      多克隆

    • 抗体英文名

      Caspase 10 Antibody

    • 抗体名

      Caspase 10 Antibody

    • 浓度

      1mg/ml

    • 保存条件

      低温

    • 规格

      50ul

    Caspase 10 Antibody

    名 称:
    Caspase 10 抗体
    货 号:
    AF0122
    来 源:
    Rabbit
    应 用:
    WB,IHC,IF/ICC,ELISA
    反 应:
    Human, Mouse
    预 测:
    Horse(100%), Rabbit(86%), Xenopus(86%)
    蛋白号:
    Q92851
    分子量:
    60kDa
    浓 度:
    1mg/ml
    Alternative Names:Expand▼

    ALPS2; Apoptotic protease Mch-4; CASP 10; CASP-10; CASP10; CASPA_HUMAN; Caspase 10 apoptosis related cysteine peptidase; Caspase-10 subunit p12; FADD like ICE2; Fas associated death domain protein; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE 2; FLICE2; ICE like apoptotic protease 4; ICE-like apoptotic protease 4; Interleukin 1B converting enzyme 2; MCH 4;

    Applications:

    WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Reactivity:

    Human, Mouse

    Predicted Reactivity:

    Horse(100%), Rabbit(86%), Xenopus(86%)

    Source:

    Rabbit

    Clonality:

    Polyclonal

    Purification:

    The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

    Specificity:

    Caspase 10 antibody detects endogenous levels of total Caspase 10.

    Format:

    Liquid

    Concentration:

    1mg/ml

    Storage Condition and Buffer:

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

    Reference Citations:

    1). Li H et al. MicroRNA-181a regulates epithelial-mesenchymal transition by targeting PTEN in drug-resistant lung adenocarcinoma cells. Int J Oncol 2015 Oct;47(4):1379-92 (PubMed: 26323677)
    Western blot analysis of extracts from mouse brain and HepG2, using Caspase 10 Antibody.
    AF0122 Caspase 10 Antibody - C-terminal IHC human liver carcinoma tissue
    AF0122 at 1/200 staining human liver carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
    AF0122 Caspase 10 Antibody - C-terminal IF/ICC A431
    AF0122 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.

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    图标文献和实验
    相关实验
    • 【求助】关于caspase-3的活化

      Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit

    • Caspase-3活性的检测

      Caspase-3及对底物多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]等的裂解。方法:收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭,室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T(含0.05% Tween 20的TBS)洗3次,5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次

    • 【求助】caspase-3检测问题

      浓度的值差不多。 问题 1. 我的control孔的相对活化程度也有2,这是不是说明细胞状态不好呢。我的细胞密度是4*10^6,6孔板2mL 2. 我这个结果可信不?还是可能因为我们的化合物还有其他非基于caspase-3的凋亡途径呢? 求解答。谢谢、 筱七 您好,请问您做出来了吗?我也是用凯基的,测出来的值很小很小。我用六孔板养的细胞,测出来的蛋白量远超过了200ug,所以当时取了200ug测的,但是值好小呀。问凯基

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