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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 抗体英文名:
Caspase 10 Antibody
- 抗体名:
Caspase 10 Antibody
- 浓度:
1mg/ml
- 保存条件:
低温
- 规格:
50ul
名 称:
Caspase 10 抗体
货 号:
AF0122
来 源:
Rabbit
应 用:
WB,IHC,IF/ICC,ELISA
反 应:
Human, Mouse
预 测:
Horse(100%), Rabbit(86%), Xenopus(86%)
蛋白号:
Q92851
分子量:
60kDa
浓 度:
1mg/ml
Alternative Names:Expand▼
ALPS2; Apoptotic protease Mch-4; CASP 10; CASP-10; CASP10; CASPA_HUMAN; Caspase 10 apoptosis related cysteine peptidase; Caspase-10 subunit p12; FADD like ICE2; Fas associated death domain protein; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE 2; FLICE2; ICE like apoptotic protease 4; ICE-like apoptotic protease 4; Interleukin 1B converting enzyme 2; MCH 4;
Applications:
WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Reactivity:
Human, Mouse
Predicted Reactivity:
Horse(100%), Rabbit(86%), Xenopus(86%)
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Specificity:
Caspase 10 antibody detects endogenous levels of total Caspase 10.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reference Citations:
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文献和实验Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit
的Caspase-3及对底物多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]等的裂解。方法:收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭,室温1.5~2h或4°C过夜→Caspase-3多抗或单抗室温反应1~2h或4°C过夜→TBS-T(含0.05% Tween 20的TBS)洗3次,5~10min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS-T洗3次
浓度的值差不多。 问题 1. 我的control孔的相对活化程度也有2,这是不是说明细胞状态不好呢。我的细胞密度是4*10^6,6孔板2mL 2. 我这个结果可信不?还是可能因为我们的化合物还有其他非基于caspase-3的凋亡途径呢? 求解答。谢谢、 筱七 您好,请问您做出来了吗?我也是用凯基的,测出来的值很小很小。我用六孔板养的细胞,测出来的蛋白量远超过了200ug,所以当时取了200ug测的,但是值好小呀。问凯基
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