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文献和实验How Do I make an Antibody against a Peptides
If your peptide is synthesized with a terminal cysteine residue (i.e., a free sulfhydryl group, -SH), you can use any maleimide-activated carrier protein to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre
were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
Characterization of Glycosaminoglycans by Capillary Electrophoresis
to a D-glucosamine (either N -sulfo or N -acetyl-d -glucosamine) residue. Both saccharide residues can be O -sulfonated at a variety of different positions, making these glycosaminoglycans structurally complex. Hyaluronic acid contains a simple repeating
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