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- 2026年01月23日
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- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
广州市左克生物
- CAS号：
2735814-23-8
- 规格：
1mg(6.89mM*100μLinMethylacetate)
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文献和实验-H2AX Detection in Somatic and Germ Cells of Mice
The phosphorylation of histone H2AX at serine 139 (γ-H2AX) is one of the first steps of DNA damage response and its detection is widely used as a sensitive marker for DNA double-strand breaks induced by ionizing radiation or other genotoxic
Histone H2AX Phosphorylation: A Marker for DNA Damage
) are generated that rapidly result in the phosphorylation of histone H2A variant H2AX. Because phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA
PROCEDURE 1. Resuspend tissue/extract in 5 volumes of 1:1 chloroform/methanol by vortexing (to homogeneity or as close as possible) 2. Add the same volume of a 1M KCl, 0.2M Phosphoric acid
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