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- 武汉
- 2026年01月22日
- FCM
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PE/Cyanine 5 Anti-Human CD32 Antibody[AT10]
- 应用范围：
FCM
- 宿主：
Mouse
- 库存：
99
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态：
液体
- 亚型：
Mouse IgG1, κ
- 规格：
100Tests/20Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥3600.0 |
|---|---|---|---|
| 规格： | 20Tests | 产品价格： | ￥1464.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥6480.0 |
PE/Cyanine 5 Anti-Human CD32 Antibody[AT10]
| Alternate Names |
FCR II, FcγRII
|
| Cellular Localization |
Membrane
|
| Clone No |
AT10
|
| Isotype |
Mouse IgG1, κ
|
| Background |
CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).
|
| Abbre |
CD32
|
| Swissprot |
P31995
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Clonality |
Monoclonal
|
| Applications |
FCM
|
| Form |
Liquid
|
| Concentration |
5 μL/Test
|
| Conjugation |
PE/Cyanine 5
|
| Conjugation Information |
PE/Cyanine 5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration Date |
12个月
|
| Shipping |
冰袋
|
| Gene ID |
2212
|
PE/Cyanine 5 Anti-Human CD32 Antibody[AT10]Elabscience®拥有先进的单抗，多抗和流式抗体开发技术平台，提供8500+高品质抗体， 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证，包含 流式细胞术（Flow Cytometry）、 免疫印迹（WB）、 免疫组化（IHC/ICC）、 免疫（共）沉淀（IP/Co-IP）、 免疫荧光（IF） 等实验。抗体产品涉及多种生命科学研究领域，包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类，以保障并加快您的研究进程。
PE/Cyanine 5 Anti-Human CD32 Antibody[AT10]This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
PE/Cyanine 5 Anti-Human CD32 Antibody[AT10]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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