- ￥960 - 3000
- Elabscience
- AN00429UJ
- 武汉
- 2026年01月30日
- FCM
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
MHC I抗体
- 抗体英文名：
PerCP/Cyanine5.5 Anti-Mouse MHC I (H-2Kd) Antibody[SF1.1.10]
- 应用范围：
FCM
- 宿主：
Mouse
- 保质期：
12个月
- 级别：
科研
- 库存：
99
- 供应商：
武汉伊莱瑞特
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态：
液体
- 亚型：
Mouse IgG2a, κ
- 规格：
100μg/25μg
| 规格： | 100μg | 产品价格： | ￥3000.0 |
|---|---|---|---|
| 规格： | 25μg | 产品价格： | ￥960.0 |
PerCP/Cyanine5.5 Anti-Mouse MHC I (H-2Kd) Antibody[SF1.1.10]
| Verified Samples |
Verified Samples in WB: SH-SY5Y
|
| Dilution |
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Synthesized peptide derived from the C-terminal region of human Enolase
|
| Abbre |
Enolase
|
| Synonyms |
2 phospho D glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, ENO2, ENOG, Eno 2, Enolase 2, Enolase 2 (gamma, Enolase 2 gamma neuronal, Enolase2, Epididymis secretory protein Li 279, Gamma enolase, Gamma-enolase, HEL S 279, Neural enolase, Neuron , neuronal)
|
| Swissprot |
P09104
|
| Calculated MW |
47 kDa
|
| Observed MW |
47 kDa
|
| Cellular Localization |
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
|
| Concentration |
1 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
|
| Purification Method |
Affinity purification
|
| Research Areas |
Cancer, Metabolism, Developmental Biology, Neuroscience, Stem Cells, Tags & Cell Markers
|
| Conjugation |
Unconjugated
|
| Background |
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival
|
PerCP/Cyanine5.5 Anti-Mouse MHC I (H-2Kd) Antibody[SF1.1.10]Elabscience®拥有先进的单抗，多抗和流式抗体开发技术平台，提供8500+高品质抗体， 适用于胞内和胞外蛋白的鉴定、定量和定位研究。抗体已在多种应用中得到验证，包含 流式细胞术（Flow Cytometry）、 免疫印迹（WB）、 免疫组化（IHC/ICC）、 免疫（共）沉淀（IP/Co-IP）、 免疫荧光（IF） 等实验。抗体产品涉及多种生命科学研究领域，包括肿瘤、免疫、心血管、细胞生物学、神经科学、表观遗传学等。Elabscience®始终致力于提高抗体的质量和种类，以保障并加快您的研究进程。
PerCP/Cyanine5.5 Anti-Mouse MHC I (H-2Kd) Antibody[SF1.1.10]This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
PerCP/Cyanine5.5 Anti-Mouse MHC I (H-2Kd) Antibody[SF1.1.10]产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
浓度为 1x107cells/ 100 μl，从中取 1 - 2x106 cells； 2． 用去离子水将 10xBuffer IS 稀释为 1x，准备 3 ml 稀释后的 Buffer IS； 3．取 1ul Strep-Jactin®PE 或 Strep-Tactin®APC，0.8μl MHC I-Strep，与 1x Buffer IS 混匀为 10ul 的终体积，冰上孵育 45 分钟； 4．将上一步孵育完的 Streptamers 复合物加入到细胞悬液中轻轻吹打混匀，冰上孵育 45 分钟
Anti-GFP/BrdU In Situ Immunostaining
of 2 N HCl x30'. Wash 3x in PBS. Block in 1 mL 10% Horse serum, x30' at room temp. Wash 1x in PBS. Add 0.3 mL Biotinylated anti-BrdU (1:300, with 1% horse serum in PBS). Incubate at 4ºC o.n. or 2 hr. at room temp. Wash 3x in PBS.
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
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