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- Elabscience
- E-AB-60228
- 湖北武汉
- 2025年07月16日
- WB;IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
ASC / TMS1 Polyclonal Antibody_货号:E-AB-60228_多克隆抗体
- 抗体英文名：
ASC / TMS1 Polyclonal Antibody
- 应用范围：
WB;IF
- 宿主：
Rabbit
- 适应物种：
Human;Mouse;Rat
- 保质期：
12个月
- 目录编号：
E-AB-60228
- 级别：
科研级别
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
- 形态：
液体
- 亚型：
IgG
- 规格：
200μL/120μL/60μL
| 规格： | 200μL | 产品价格： | ￥3580.0 |
|---|---|---|---|
| 规格： | 120μL | 产品价格： | ￥2220.0 |
| 规格： | 60μL | 产品价格： | ￥1380.0 |
货号：E-AB-60228
| Verified Samples |
Verified Samples in WB:A549,THP-1,HT29,BT-474
Verified Samples in IF: HeLa |
| Dilution |
WB 1:500-1:2000, IF 1:50-1:200
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Recombinant fusion protein of human ASC / TMS1 (NP_037390.2).
|
| Abbre |
ASC / TMS1
|
| Synonyms |
PYCARD;ASC;CARD5;TMS;TMS-1;TMS1;PYD and CARD domain containing;ASC / TMS1抗体
|
| Swissprot |
Q9ULZ3
|
| Calculated MW |
15kDa/19kDa/21kDa
|
| Observed MW |
22kDa
|
| Cellular Localization |
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
|
| Concentration |
1mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|
| Purification Method |
Affinity purification
|
| Research Areas |
Cancer; Cell Biology
|
| Conjugation |
Unconjugated
|
| Background |
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
|
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, and apply primary Ab only to the coverslip. Incubate 45'. Dilute antibody with Blotto. Some typical dilutions are listed below: monoclonal M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; polyclonal 12CA5 mouse anti-HA (Roche
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antibody unconjugated； 货号21337，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号3052，产品描述：Rabbit anti-Human IgG secondary antibody HRP conjugated； 货号300904050，产品描述：One-Hour Western kit，10 ml / bottle x 3；
Staining for Surface Receptors
polyclonal 5. Treat cells. Typical incubation is approximately 25'. 6. Quickly aspirate media and add fixative (~ 2 mL/well). Fix for 15-20'. 7. Wash 3x. 8. Aspirate the final wash, taking care to dry the edges of the coverslip well. Add
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