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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human NCAM-1/CD56 Protein (Site-Specific AF 647 Conjugation, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
25.00 µg/100.00 µg
| 规格: | 25.00 µg | 产品价格: | ¥3480.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥11180.0 |
蛋白名称:Human Human NCAM-1/CD56 Protein (Site-Specific AF 647 Conjugation, His Tag)
蛋白构建:A DNA sequence encoding the human NCAM1 (NP_001070150.1) (Met 1-Pro 603) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主:HEK293 Cells
蛋白活性:1.Flow cytometric analysis of anti-NCAM1 CAR expression. 5e5 of anti-NCAM1 CAR-293 cells were stained with 100 μL of 1 μg/mL of AF 647-conjugated human NCAM-1/CD56 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10673-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated NCAM1 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human NCAM-1/CD56 Protein (Site-Specific AF 647 Conjugation, His Tag, Cat. No. 10673-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Leu 20
蛋白分子量:The protein has a predicted molecular mass of 68.4 kDa.
蛋白NP号:NP_001070150.1
蛋白氨基酸序列:Met1-Pro603
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文献和实验flow field-flow fractionation (AF4) conditions that have proven their reliability for the analysis of quantum dots and other nanoparticles in the 5–50 nm size range. Common pitfalls are emphasized together with strategies to overcome them.
等) 可以完成一般方法很难完成的分离,如变性和非变性蛋白、功能不同的蛋白的分离 属于一种吸附层析,载量高,具有高效的捕获和浓缩作用。 基本步骤 亲和层析作为一种吸附性层析,主要步骤包括平衡、上样、清洗、洗脱和再平衡步骤 (图10)。由于亲和作用需要在特定的条件下结合和洗脱,亲和层析需要使用特定的结合缓冲液和洗脱缓冲液。 图10 亲和层析的基本步骤 适用性 带有标签的融合蛋白纯化,如His标签蛋白、GST标签蛋白等。 抗体及抗体片段的纯化 其他和某些配基有特异性结合作用的分子的纯化
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,蛋白质的单一修饰已经不能够满足当前需求,比如偶联单一类型抗癌药物的 ADC 仍然存在疗效低、耐药等问题。因此蛋白质的双修饰或者多修饰在实现多功能蛋白药物的开发方面变得尤为重要。目前实现蛋白质多修饰,尤其是定点多修饰策略研究相对较少,是亟待解决的科学难题。 针对以上缺陷,2023 年 2 月 21 日,北京大学药学院刘涛及北京大学第一医院杨兴共同通讯在 Nature Communications 在线发表了题为 Noncanonical amino acids as doubly bio
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