- ￥3900
- biorbyt
- 0.32-20 ng/mL
- 0.128 ng/mL
- orb780467
- 英国
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 应用：
ELISA
- 适应物种：
Mouse
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.128 ng/mL
- 规格：
48 T
产品别名：PCB ELISA Kit
应用笔记：standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PC in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长：3.5h
Note：For research use only.
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Pyruvate dehydrogenase complex (PDC) and pyruvate carboxylase (PC) are mitochondrial enzymes that provide the initial steps of the two main alternatives for pyruvate metabolism: oxidative decarboxylation vs. anaplerotic carboxylation
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