Human Zymogen Granule Protein

类别: Assays and Kits

描述: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zymogen Granule Protein 16 Homolog B(ZG16B).

别名: JCLN2 ELISA Kit, Jacalin-Like Lectin Domain Containing 2 ELISA Kit

浓度: 20 ng/mL

应用说明: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZG16B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ZG16B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ZG16B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ZG16B in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间: 3.5h

研究领域: Signal Transduction

UniProt ID: Q96DA0

备注: For research use only.