Human Lipase, Hepatic (LIPC) E

类别: Assays and Kits

描述: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hepatic(LIPC).

别名: HL ELISA Kit, HTGL ELISA Kit, Hepatic Lipase ELISA Kit, Hepatic triacylglycerol lipase ELISA Kit, Lipase member C ELISA Kit

浓度: 5000 pg/mL

应用说明: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LIPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LIPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LIPC in the samples is then determined by comparing the OD of the samples to the standard curve

检测时间: 3.5h

研究领域: Metabolism Research; Gastroenterology & Hepatology

UniProt ID: P11150

备注: For research use only.