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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
78.13-5000 pg/mL
- 检测方法:
Sandwich
- 应用:
ELISA
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
35 pg/mL
- 规格:
48 T
产品别名:HL ELISA Kit, HTGL ELISA Kit, Hepatic Lipase ELISA Kit, Hepatic triacylglycerol lipase ELISA Kit, Lipase member C ELISA Kit
应用笔记:standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LIPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LIPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LIPC in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:3.5h
UniProt ID:P11150
Note:For research use only.
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文献和实验High-Level Expression and Purification of Human Hepatic Lipase from Mammalian Cells
Through its ability to catalyze the hydrolysis of triglycerides and phospholipids, hepatic lipase (HL) has been shown to influence the metabolism of a broad range of circulating lipoproteins (1 ). HL is synthesized and secreted
Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13 ). Finally, recent studies with cultured hepatoma cells over-expressing human HL
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols







