Rat Platelet Membrane Glycopro
tein Ⅱb/Ⅲa complex (GPⅡb/Ⅲa) ELISA Kit试剂盒，orb1948299，biorbyt- ￥3770
- biorbyt
- 0.63-40ng/mL
- 0.38 ng/mL
- orb1948299
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
0.63-40ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
Tissue homogenate and Cell lysate
- 灵敏度：
0.38 ng/mL
- 规格：
48 T
产品别名：integrin αⅡbβ3
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GPⅡb/Ⅲa. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GPⅡb/Ⅲa and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GPⅡb/Ⅲa, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GPⅡb/Ⅲa. You can calculate the concentration of Rat GPⅡb/Ⅲa in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
靶点：GPⅡb/Ⅲa
Note：For research use only.
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文献和实验h,同上收集培养上清液 用双抗体夹心ELISA 方法测HSC-T6培养上清液中PⅢP ，CⅣ的含量。以上每组均设3个复孔，具体操作严格按照试剂盒说明进行。 1.3 统计学方法 采用SPSS12.0统计软件，进行Spearman相关分析，单因素ANOVA(方差分析)，两样本比较采用LSD-t检验，实验数据均以(x±s)表示,P＜0.05判定有显著性差异。 2 结果 2.1 HMGB1对 HSC-T6分泌PⅢP、CⅣ的剂量效应 HMGB1能促进
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