- ￥3510
- biorbyt
- 1.57-100mIU/mL
- 0.94 mIU/mL
- orb1947730
- 英国
- 2026年01月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
biorbyt
- 检测范围：
1.57-100mIU/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, Tissue homogenate and Other biological samples
- 灵敏度：
0.94 mIU/mL
- 规格：
48 T
产品别名：DBH, DBM, Dopamine beta-monooxygenase, dopamine beta-hydroxylase, Dopamine β-hydroxylase
应用笔记：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DβH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DβH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DβH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DβH. You can calculate the concentration of Human DβH in the samples by comparing the OD of the samples to the standard curve.
实验时长：3.5H
UniProt ID:P09172
靶点：DβH
Note：For research use only.
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
, Bret A., 1995 41.Signal Transduction Protocols, 1st. Edited by D. A. Kendall, 1995 42. ELISA Theory and Practice. Edited by Crowther, John R., 1995 43. In Vitro Toxicity Testing Protocols, Edited by O'Hare, Sheila, 1995 44. Agrobacterium
在相同的酶。另外，它的相应底物易于制备和保存，价格低廉，有色产物易于测定等。 在ELISA中，常用的酶为辣根过氧化物酶(horseradish peroxidase, HRP)和碱性磷酸酶(alkaline phosohatase, AP)。在少数商品ELISA试剂中，应用的酶尚有葡萄糖氧化酶、β-D-半乳糖苷酶和脲酶等。国产ELISA试剂一般都用HRP制备结合物。HRP是一种糖蛋白，含糖量约为18%，分子量为44000，是一种复合酶，由主酶（酶蛋白）和辅基（亚铁血红素）结合而成，是一种卟啉蛋白
Uptake and Release of Neurotransmitters
., Tanen, D., and Roth, R. 1990. Complex interaction of cocaine with the dopamine uptake carrier. Eur. J. Pharmacol. 176:251‐252. Blakely, R.D., Berson, H., Fremeau, R.T., Caron
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