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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human GTPase HRas (HRAS), partial, Biotinylated
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human GTPase HRas (HRAS), partial, Biotinylated品名简称:
Recombinant Human HRAS protein, partial, Biotinylated货号:
CSB-EP010726HU-B规格:
20μg价格:
2208LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
HRASUniprot No.:
P01112别名:
H-Ras-1;Ha-Ras;Transforming protein p21;c-H-ras;p21ras种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
68.6 kDa表达区域:
2-186aa氨基酸序列:
TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKC蛋白标签:
N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity功能2:
/文献:
/研究领域:
Cell Biology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验肝刺激因子对人肝癌细胞BEL-7402 p21ras表达的影响
15,16]。EGFR具有酪氨酸激酶活性, 其活化后可诱导形成包括Ras在内的复杂的细胞信号传导通路, 最终导致细胞增殖[24]。 p21ras蛋白位于细胞膜内表面, 本身具有GTPase活性, 兼有效应分子和调节分子的共同特点, 它可接受多种生长因子的作用而活化, 调节如丝裂素活化蛋白激酶(mitogen-activated protein kinase, MAPK)的活性, 是信号传导通路中的关键一环[25]。Gupta等初步观察到, HSS能诱导S期进展基因c-Ha-ras、H3组蛋白
直接检测癌基因有两种方法:一种是从动物肿瘤中提取 DNA 转染正常细胞使其转化,已确立的小鼠 NIH3T3 纤维瘤细胞株也可作为受体细胞。另一种方法将这种提取的 DNA 注入裸鼠(无免疫能力的一种小鼠)可形成肿瘤。用以上的方法就可用来检测 c-ras 家族的 onc 。 ras 癌基因最初是在两种大鼠的反转录病毒 Ha-Musr 和 Ki-Musy 中发现的。在人和大鼠的基因组中相应的 C-ras,,N-ras,H-ras 和 K-ras
, IkappaB, NFkappaB. ERK is activated by LPS activated TLFR4 through Raf1. LPS activated TLFR4 activates the Raf1/MEK/ERK pathway both by p21Ras-GTPase-dependent and Ras-independent mechanisms. Lipopolysaccharides (LPS) are major Cell wall
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