/S重组蛋白Spike glycoprotein(S gly

英文名称：

Recombinant Avian infectious bronchitis virus Spike glycoprotein (S), partial

品名简称：

Recombinant Avian infectious bronchitis virus S protein, partial

货号：

CSB-EP320142ARP

规格:

20μg

价格：

2328

LC-MS/Western Blot：

/

SDS-PAGE：

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

纯度：

Greater than 85% as determined by SDS-PAGE.

内毒素：

Not test

生物活性：

/

基因名：

S

Uniprot No.：

P12722

别名：

Spike glycoprotein(S glycoprotein)(E2)(Peplomer protein) [Cleaved into: Spike protein S1; Spike protein S2; Spike protein S2']

种属：

Avian infectious bronchitis virus (strain D274) (IBV)

蛋白长度：

Partial

来源：

E.coli

分子量：

27.2 kDa

表达区域：

318-538aa

氨基酸序列：

ASDYMYGSYHPSCKFRLETINNGLWFNSLSVSLGYGPIQGGCKQSVFANRATCCYAYSYNGPSLCKGVYRGELTKSFECGLLVFVTKTDGSRIQTRNEPFTLTQHNYNNITLDRCVEYNIYGRVGQGFITNVTNYAINYNYLADGGMAILDTSGAIDIFVVQGEYGLNYYKVNPCEDVNQQFVVSGGKLVGILTSRNETGSQPLENQFYIKIINGTRRSRR

蛋白标签：

C-terminal 6xHis-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

储存条件：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能1：

[Spike protein S1]: attaches the virion to the host cell membrane by interacting with sialic acids, initiating the infection.; [Spike protein S2]: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; [Spike protein S2']: Acts as a viral fusion peptide after S2 cleavage occurring upon virus endocytosis.

功能2：

/

文献：

"Nucleotide sequence of the gene coding for the peplomer protein (= spike protein) of infectious bronchitis virus, strain D274." Jordi B.J.A.M., Kremers D.A.W.M., Kusters H.G., van der Zeijst B.A.M. Nucleic Acids Res. 17:6726-6726(1989)

研究领域：

Microbiology

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