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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Arabidopsis thaliana EPIDERMAL PATTERNING FACTOR-like protein 9 (EPFL9)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Arabidopsis thaliana EPIDERMAL PATTERNING FACTOR-like protein 9 (EPFL9)品名简称:
Recombinant Mouse-ear cress EPFL9 protein货号:
CSB-EP879855DOA规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
EPFL9Uniprot No.:
Q9SV72别名:
EPF-like protein 9 (EPFL9) (STOMAGEN) (At4g12970) (F25G13.60)种属:
Arabidopsis thaliana (Mouse-ear cress)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
14.2 kDa表达区域:
32-102aa氨基酸序列:
SRPRSIENTVSLLPQVHLLNSRRRHMIGSTAPTCTYNECRGCRYKCRAEQVPVEGNDPINSAYHYRCVCHR蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Stomagen: Positively regulates stomatal density and patterning. Acts by competing with EPF2 for the same receptors, ERECTA and TMM . Not cleaved by the protease CRSP .功能2:
/文献:
"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome." Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., Town C.D. Plant J. 89:789-804(2017)研究领域:
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文献和实验,我们用已知的人工合成 MAP 作为阳性对照,分析研究拟南芥的不同 MAP 激酶 [23] 。 ( 4 ) 重组蛋白质除了含有各自的蛋白质的编码序列外,常常还包含由克隆载体所编码的 3' 或 5' 标记物。这些标记物的磷酸化可能会导致假阳性结果,因此在芯片实验之前要将其排除掉。用一些已知的不被认为是相应激酶底物,并且具有相同表达模式(相同标记物)的重组蛋白质,在溶液中进行活性激酶的检测实验可能是一种解决问题的办法。在这个检测实验中,这些所选择的蛋白质应该显示阴性
N a t. G en et. 2 6, 283-289. 10. Boutell, J. M., Hart, D. J., Godber, B. L., Kozlowski, R. Z., and Blackburn, J. M. (2004) Functional protein microarrays for parallel characterisation of p53 mutants. P r o te o m ic s 4, 1950-1958. 11.
【整理】蛋白版2006年06月无人应助帖,请广大战友积极应助,加分从优!
&sty=1&tpg=10&age=0 【求助】细胞微粒体的方法 http://www.dxy.cn/bbs/post/view?bid=65&id=6436513&sty=1&tpg=10&age=0‘ 【求助】寻求提酵母RNA的方法 http://www.dxy.cn/bbs/post/view?bid=65&id=6436249&sty=1&tpg=10&age=0 [求助]:G protein-coupled receptor属于什么类型的蛋白 http://www.dxy.cn
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