- ￥7040
- SIGMA
- 美国
- S9305-.5ML
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
见瓶身
- 英文名：
SYBR? Green II RNA gel stain
- 库存：
999
- 供应商：
麦格生物
- CAS号：
172827-25-7
- 规格：
0.5ml
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文献和实验Denaturing Agarose Gel Electrophoresis of RNA
, such as those from needle biopsies or from laser capture microdissected samples, result in very low yields. In these cases, it may be impossible to spare 200 ng of RNA to assess integrity. Alternative nucleic acid stains, such as SYBR® Gold and SYBR® Green II RNA gel stain
Agarose Gel Electrophoresis of RNA
on a denaturing gel, as little as 0.5X Formaldehyde Load Dye can be used, but to completely denaturate the RNA, e.g. for Northern blots, use 3X volumes of Formaldehyde Load Dye. ii. Ethidium bromide can be added to the Formaldehyde Load Dye
Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
II. Transfer of RNA from Gel to Membrane Prepare gel for transfer : Place the gel in an RNase-free dish and rinse with changes sufficient deionized water to cover the gel for 4x20 min. Transfer RNA
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