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- 技术资料
- 规格:
1*125mL/500mL
| 规格: | 1*125mL | 产品价格: | ¥270.0 |
|---|---|---|---|
| 规格: | 500mL | 产品价格: | ¥800.0 |

产品介绍
SF763细胞专用培养基由我们精心优化,经过长期测试,本产品可保持SF763细胞最佳的生长状态。
本产品中已包含SF763细胞生长所需的各种成分,无需添加任何成分,可直接用于SF763细胞的培养。
产品主要成分
MEM 基础培养基 (iCell-0012) 445mL
特级胎牛血清 (iCell-0500) 50 mL
P/S青霉素-链霉素 (iCell-15140-122) 5 mL
运输和保存
运输:放置于含有生物冰袋的保温箱中低温运输
保存方法:2℃~8℃,保存2个月;
质量控制
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检测项目 |
质量控制 |
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澄清度 |
澄清 |
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PH |
7.3±0.2 |
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内毒素含量 (EU/mL) |
≤10 |
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无菌检测 |
细菌 |
阴性 |
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真菌 |
阴性 |
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支原体 |
阴性 |
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细胞生长试验 |
细胞形态 |
正常 |
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细胞生长实验 |
合格 |
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文献和实验Superior anti-swelling and durably lubricious bio-hydrogels via robust crystalline domain construction for diverse biodevice coating Weiyi Zhao, Yunlei Zhang, Xiaoduo Zhao, Bo Yu, Licheng Zhang, Shuanhong Ma, Feng Zhou
Native purification of His-tagged Proteins from Sf9 cells
Protocol is for 150 ml of cell culture. 1. Spin down cells and wash 1X in 10 ml ice cold PBS. 2. Resuspend pellet in 20 ml lysis buffer: 50 mM Tris pH 8.0 1 % NP-40 1 mM PMSF 1 µg/ml leupeptin 3. Transfer to oakridge tubes and sonicate
GPCR Expression Using Baculovirus-Infected Sf9 Cells
promoter-driven expression of a C-terminal 6�histidine-tagged mammalian M2 muscarinic receptor in Sf 9 cells. Results demonstrate that expressed receptor could be detected and quantified using radiolabeled ligand binding, that expression was maximal
Purification of Recombinant p53 from Sf9 Insect Cells
We describe a method for purifying recombinant p53 from baculovirus infected cells in one step by anion exchange chromatography. The p53 is full-length with no flanking sequences and its expression is driven by the baculovirus polyhedron
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