小鼠降钙素原 ELISA定量试剂盒(Pro-Calciton

EliKine™ 小鼠降钙素原 (PCT) ELISA 定量试剂盒 (一步法)

货号：KTE4037-1

产品概述

EliKine™ 小鼠降钙素原 (Procalcitonin, PCT) ELISA 定量试剂盒采用先进的双抗体夹心一步法原理，专为精准定量小鼠血清、血浆、组织匀浆及细胞培养上清等液体样本中的降钙素原含量而设计。本试剂盒隶属于细胞分析领域的核心产品线，是进行ELISA相关研究的理想工具。

核心特性与优势

• 高灵敏度与特异性：检测灵敏度高达 0.3 pg/mL，确保对低丰度样本的准确检测。经过严格验证，与目标蛋白的类似物无明显交叉反应，有效降低背景干扰。
• 宽线性检测范围：提供 1.5 pg/mL 至 120 pg/mL 的宽泛检测范围，满足不同浓度样本的检测需求。
• 便捷的一步法操作：优化后的实验流程减少了操作步骤，有助于提高实验效率并降低误差。
• 即用型组分：试剂盒提供预先包被好的微孔板及所有必要的即用型试剂，开盒即可开始实验，节省准备时间。

检测原理

本试剂盒基于双抗体夹心ELISA原理。预包被于酶标板上的特异性抗体捕获样本中的小鼠PCT，随后依次与生物素化的检测抗体及链霉亲和素-HRP结合，形成免疫复合物。加入TMB底物显色后，颜色的深浅与样本中的PCT浓度成正比，通过酶标仪在450 nm波长下测定吸光度(OD值)即可进行定量分析。

试剂盒组分

• 小鼠PCT预包被微孔板 (Mouse PCT Microplate)
• 小鼠PCT标准品 (240 pg/mL) (Mouse PCT Standard)
• 标准品稀释液 (Standard Diluent)
• 小鼠PCT检测抗体 (Mouse PCT Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A (HRP Substrate A)
• HRP底物B (HRP Substrate B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

规格与订购信息

保存与运输

保存建议：未启封的试剂盒请避光保存于2-8℃，有效期为6个月。启用后，请参照说明书分别保存各组分。

运输条件：全程蓝冰冰袋运输，确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定，对于因使用本产品可能导致的专利侵权或其他违规行为，生产商不承担任何责任。

别名：Pro-Calcitonin

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Procalcitonin (PCT) ELISA Quantitative Kit (One-Step)

Catalog Number: KTE4037-1

Product Overview

The EliKine™ Mouse Procalcitonin (PCT) ELISA Quantitative Kit is based on the advanced principle of a double-antibody sandwich one-step method. It is specifically designed for the accurate quantification of Procalcitonin levels in liquid samples such as mouse serum, plasma, tissue homogenates, and cell culture supernatants. This kit belongs to the core product line within the Cell Analysis field and is an ideal tool for ELISA-related research.

Core Features & Advantages

• High Sensitivity and Specificity: A detection sensitivity as high as 0.3 pg/mL ensures accurate detection of low-abundance samples. Rigorously validated to show no significant cross-reactivity with analogs of the target protein, effectively reducing background interference.
• Wide Linear Detection Range: Provides a broad detection range of 1.5 pg/mL to 120 pg/mL, meeting the detection needs of samples with varying concentrations.
• Convenient One-Step Operation: The optimized experimental procedure reduces operational steps, helping to improve experimental efficiency and reduce errors.
• Ready-to-Use Components: The kit provides pre-coated microplates and all necessary ready-to-use reagents, allowing experiments to begin immediately after opening, saving preparation time.

Detection Principle

This kit is based on the double-antibody sandwich ELISA principle. The specific antibody pre-coated on the microplate captures mouse PCT from the sample, which then sequentially binds with the biotinylated detection antibody and Streptavidin-HRP to form an immune complex. After adding the TMB substrate for color development, the intensity of the color is proportional to the concentration of PCT in the sample. Quantitative analysis is performed by measuring the optical density (OD value) at 450 nm using a microplate reader.

Kit Components

• Mouse PCT Pre-coated Microplate
• Mouse PCT Standard (240 pg/mL)
• Standard Diluent
• Mouse PCT Detect Antibody
• Streptavidin-HRP
• HRP Substrate A
• HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Covers

Specifications & Ordering Information

Storage & Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2-8℃; the shelf life is 6 months. After opening, please store the components separately according to the instructions.

Shipping Conditions: Shipped with blue ice packs to ensure reagent activity.

Important Notes

• Do not mix reagents from different lot numbers or expired reagents.
• All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
• To avoid cross-contamination, always use disposable pipette tips during the experiment.
• Unused strips should be immediately returned to the original packaging bag, sealed, and stored at 4℃.
• Thorough mixing is crucial for experimental results; it is recommended to use a low-speed shaker or perform regular manual shaking.
• For more reliable data, it is recommended to perform replicate (duplicate or triplicate) testing for samples and standards.

Warning: This product is for research use only and is not intended for human or clinical diagnosis. The user should ensure that its application does not violate any patent or license agreements. The manufacturer assumes no liability for any patent infringement or other violations that may result from the use of this product.

Alias: Pro-Calcitonin

Note: The above information may change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.