小鼠血清淀粉样蛋白A ELISA定量试剂盒(SAA)

产品概述

货号：KTE3042-1 | 产品名称：小鼠血清淀粉样蛋白A ELISA定量试剂盒 (Mouse SAA ELISA Kit)

本试剂盒是一款基于双抗体夹心法原理的高性能ELISA检测工具，专为精准定量小鼠血清、血浆及细胞培养上清样本中的血清淀粉样蛋白A (SAA) 而设计。作为细胞分析领域的核心产品，它凭借其卓越的检测性能，成为炎症反应、急性期蛋白研究及相关疾病模型构建中的理想选择。

主要特性与优势

• 高灵敏度与宽检测范围：最低检测浓度低至0.16 ng/mL，检测范围覆盖0.16 ng/mL 至 10 ng/mL，能够精准捕捉样本中SAA的细微浓度变化。
• 出色的特异性：采用精心筛选的配对抗体，经验证，对小鼠SAA目标蛋白具有高度特异性，与类似物无明显交叉反应或背景干扰，确保检测结果的可靠性。
• 操作便捷，结果稳定：优化的试剂配方和反应体系，提供清晰的实验步骤，有助于获得重复性高、线性关系好的实验结果。
• 即用型完整组分：试剂盒提供包括预包被微孔板、冻干标准品、检测抗体、HRP底物等在内的全部必需组分，开盒即可使用，节省准备时间。

检测原理简介

本试剂盒采用经典的双抗体夹心ELISA法。预包被于孔板上的特异性抗体捕获样本中的小鼠SAA，随后依次加入生物素化的检测抗体和链霉亲和素-HRP偶联物，形成复合物。加入TMB底物显色，反应终止后，通过测定OD450nm处的吸光度值，即可根据标准曲线精确计算出样本中SAA的浓度。

产品组分详情

组分名称	规格/状态
Mouse SAA Pre-coated Microplate	48T或96T
Mouse SAA Standard (冻干标准品)	2支
10× Assay Buffer (检测缓冲液)	1瓶
100× Mouse SAA Detect Antibody (生物素化检测抗体)	1瓶
100× Streptavidin-HRP (链霉亲和素-HRP)	1瓶
HRP Substrate (TMB) (底物溶液)	1瓶
Stop Solution (终止液)	1瓶
20× Wash Buffer (洗涤缓冲液)	1瓶
Plate Covers (板贴)	适量

规格与价格

规格	价格 (人民币)	货期
48 T	1,598.0	请咨询
96 T	2,558.0	期货 (预计5个工作日内发货)

重要操作提示

• 请务必按照说明书指引，在使用前将所有试剂组分室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 标准品及样品建议进行复孔检测，以提高数据的准确性。
• 充分混匀试剂及反应液对实验结果至关重要，建议使用振荡器或定期手动摇匀。
• 未使用的板条应立即放回铝箔袋并密封，于4℃保存。

储存与运输

储存：未开封的试剂盒请避光保存于2-8℃，有效期详见标签。启用后，请参考说明书分别保存各组分。

运输：本品采用冰袋（蓝冰）低温运输，以确保试剂活性。

背景知识

血清淀粉样蛋白A (SAA) 是急性期反应蛋白家族的重要成员，主要由肝脏合成，在炎症刺激下其血浆水平会显著升高。小鼠SAA基因家族包含多个成员（如SAA1, SAA2, SAA3, SAA5），在炎症性疾病、感染及代谢研究中具有重要指示意义。本试剂盒旨在为研究者提供一种稳定、灵敏的工具，以深入探索SAA在小鼠模型中的生物学功能。

警告： 本产品仅用于科学研究用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog No.: KTE3042-1 | Product Name: Mouse Serum Amyloid A ELISA Quantitation Kit (Mouse SAA ELISA Kit)

This kit is a high‑performance ELISA detection tool based on the sandwich antibody principle, specifically designed for precise quantification of mouse serum amyloid A (SAA) in serum, plasma, and cell culture supernatant samples. As a core product in cellular analysis, its outstanding analytical performance makes it an ideal choice for inflammation studies, acute‑phase protein research, and the development of related disease models.

Key Features and Advantages

• High Sensitivity and Wide Detection Range: Minimum detection limit as low as 0.16 ng/mL, detection range spanning 0.16 ng/mL to 10 ng/mL, enabling precise capture of subtle SAA concentration changes in samples.
• Excellent Specificity: Utilizes rigorously screened antibody pair that has been validated to exhibit high specificity for mouse SAA with negligible cross‑reactivity or background interference from related proteins, ensuring reliable assay results.
• User‑friendly Operation and Stable Results: Optimized reagent formulation and reaction system provide clear procedural steps, facilitating high reproducibility and robust linearity in assay outcomes.
• Ready‑to‑use Complete Components: The kit includes all essential items such as pre‑coated microplates, lyophilized standards, detection antibody, HRP substrate, etc., allowing immediate use upon opening and saving preparation time.

Assay Principle Overview

The kit employs the classic sandwich ELISA format. A specific capture antibody pre‑coated on the plate binds mouse SAA present in the sample, followed by the sequential addition of a biotinylated detection antibody and streptavidin‑HRP conjugate to form the complex. After addition of TMB substrate for color development and termination of the reaction, the absorbance at 450 nm is measured and the SAA concentration in the sample is quantified using the standard curve.

Product Component Details

Component Name	Specification/Status
Mouse SAA Pre-coated Microplate	48T or 96T
Mouse SAA Standard (Lyophilized Standard)	2 vials
10× Assay Buffer (Assay Buffer)	1 bottle
100× Mouse SAA Detect Antibody (Biotinylated Detection Antibody)	1 bottle
100× Streptavidin‑HRP (Streptavidin‑HRP)	1 bottle
HRP Substrate (TMB) (Substrate Solution)	1 bottle
Stop Solution (Stop Solution)	1 bottle
20× Wash Buffer (Wash Buffer)	1 bottle
Plate Covers (Plate Seals)	Sufficient quantity

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T	1,598.0	Please inquire
96 T	2,558.0	Future stock (estimated dispatch within 5 business days)

Critical Operational Tips

• Ensure that, as per the instructions, all reagent components are equilibrated to room temperature for approximately 30 minutes before use.
• To prevent cross‑contamination, use disposable pipette tips throughout the experiment.
• It is recommended to run standards and samples in duplicate wells to enhance data accuracy.
• Thorough mixing of reagents and reaction mixtures is critical; use a plate shaker or periodically vortex manually.
• Unused plates should be promptly returned to the aluminum foil pouch, sealed, and stored at 4 °C.

Storage and Shipping

Storage: Keep unopened kits protected from light at 2–8 °C; refer to the label for shelf‑life. After opening, store each component as instructed in the user manual.

Shipping: The product is shipped on ice packs (blue ice) under refrigerated conditions to preserve reagent activity.

Background

Serum Amyloid A (SAA) is a major acute‑phase protein primarily synthesized by the liver, with plasma concentrations markedly increasing in response to inflammatory stimuli. The mouse SAA gene family comprises several isoforms (e.g., SAA1, SAA2, SAA3, SAA5) that serve as important biomarkers in inflammatory diseases, infection, and metabolic studies. This kit provides researchers with a stable and sensitive tool to investigate the biological role of SAA in mouse models.

Warning: This product is intended for scientific research purposes only and is not for human or clinical diagnostic use. Users must ensure that its application does not infringe any patents or licensing agreements.

Note: The above information may change due to product optimization or upgrades; for the most current details, please contact Yakoin Company.