颗粒酶B酶联免疫试剂盒(小鼠) Granzyme B ELI

产品概述

英文名称：Mouse Granzyme B ELISA Kit   |   中文名称：颗粒酶B酶联免疫试剂盒(小鼠)
货号：KTE3018-3   |   检测类型：ELISA（双抗体夹心法）
反应性：小鼠   |   样本类型：细胞培养上清、血清、血浆

核心应用

• 蛋白类研究 – 颗粒酶 B 定量
• ELISA平台的细胞分析
• 小鼠模型中免疫细胞毒性及炎症相关研究

产品定位

本套件属于细胞分析方向的核心产品，专为科研机构、药企研发部门以及生物技术公司提供高灵敏度的 Granzyme B 检测解决方案。

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检测原理

采用双抗体夹心法：
1. 预包被抗小鼠 Granzyme B 抗体的酶标板捕获样品中的目标蛋白。
2. 添加生物素化的检测抗体形成双抗体复合体。
3. 链霉亲和素‑HRP 与生物素特异结合，随后加入 TMB 底物，在 HRP 催化下显色（蓝→黄），在 450 nm 读取 OD 值，实现定量。

检测范围与灵敏度

检测范围	0.31 ng/mL – 20 ng/mL
最低检出限（灵敏度）	0.31 ng/mL

关键特性 & 优势

• 高灵敏度：0.31 ng/mL 检出极低水平的 Granzyme B。
• 卓越特异性：对同源蛋白未出现显著交叉反应或背景干扰。
• 采用专利链霉亲和素‑HRP 复合物，确保信号放大且重复性好。
• 适用于细胞培养上清、血清和血浆，满足多样化实验需求。

试剂盒组成

每套完整试剂盒包含以下组件（按需配备）：

• Mouse Granzyme B Microplate
• Mouse Granzyme B Standard (lyophilized)
• Assay Buffer (10×)
• Mouse Granzyme B Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

操作要点

• 所有试剂仅能与同批号产品配合使用，避免跨批混用。
• 使用前将试剂在室温平衡 ≥30 分钟。
• 实验过程中建议使用一次性吸头，以防交叉污染。
• 未使用的板条需立即回装并密封，4 ℃保存。
• 充分混匀是获得可靠结果的关键，推荐使用低速振荡器或每 10 分钟手动轻摇一次。
• 样品和标准推荐做双孔或三孔（复孔）检测，以提升数据可靠性。

保存与运输

• 未开封试剂盒：避光保存于 2‑8 ℃，有效期 24 个月。
• 启用后：请参照说明书对各组分分别保存。
• 运输条件：冰袋（蓝冰）冷链运输，确保全程低温。

规格与报价

规格	单价 (¥)	交货周期
48 T	1 598.00	请咨询
96 T	2 558.00	期货（5 天内发货）

合规声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本试剂盒进行的任何专利或许可证相关活动需自行承担责任，供应商不承担因侵权或违规产生的任何责任。

技术背景

颗粒酶 B（Granzyme B）是细胞毒性淋巴细胞（CTL）和自然杀伤细胞（NK）颗粒中的关键丝氨酸蛋白酶，联合穿孔蛋白促使靶细胞凋亡。近年来研究显示，非细胞毒性细胞（如嗜碱性粒细胞、肥大细胞、平滑肌细胞）亦能产生 Granzyme B，参与炎症介质释放及细胞外基质重塑。Granzyme B 的升高与多种自身免疫疾病、皮肤病以及 1 型糖尿病等病理状态密切相关，因而成为免疫与炎症研究的重要指标。本套 ELISA 采用特异性双抗体夹心结构，实现对小鼠样本中 Granzyme B 的精准定量。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse Granzyme B ELISA Kit   |   Chinese Name: Granzyme B Enzyme-Linked Immunosorbent Assay Kit (Mouse)
Catalog Number: KTE3018-3   |   Assay Type: ELISA (Sandwich ELISA)
Reactivity: Mouse   |   Sample Type: Cell Culture Supernatant, Serum, Plasma

Core Applications

• Protein Research – Granzyme B Quantification
• Cell Analysis on ELISA Platform
• Research on Immune Cytotoxicity and Inflammation-Related Studies in Mouse Models

Product Positioning

This kit is a core product in the field of cell analysis, specifically designed to provide a highly sensitive Granzyme B detection solution for research institutions, pharmaceutical R&D departments, and biotechnology companies.

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Assay Principle

Utilizes the Sandwich ELISA method:
1. The microplate pre-coated with anti-mouse Granzyme B antibody captures the target protein in the sample.
2. A biotinylated detection antibody is added to form an antibody-antigen-antibody sandwich complex.
3. Streptavidin-HRP specifically binds to biotin, followed by the addition of TMB substrate. Color development (blue → yellow) is catalyzed by HRP, and the OD value is measured at 450 nm for quantification.

Detection Range & Sensitivity

Detection Range	0.31 ng/mL – 20 ng/mL
Minimum Detectable Concentration (Sensitivity)	0.31 ng/mL

Key Features & Advantages

• High Sensitivity: Detects Granzyme B levels as low as 0.31 ng/mL.
• Excellent Specificity: No significant cross-reactivity or background interference with homologous proteins observed.
• Utilizes a proprietary Streptavidin-HRP complex to ensure signal amplification and good reproducibility.
• Suitable for cell culture supernatant, serum, and plasma, meeting diverse experimental needs.

Kit Components

Each complete kit contains the following components (provided as required):

• Mouse Granzyme B Microplate
• Mouse Granzyme B Standard (lyophilized)
• Assay Buffer (10×)
• Mouse Granzyme B Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Operational Key Points

• All reagents should only be used with products from the same lot number; avoid mixing components from different lots.
• Allow all reagents to equilibrate to room temperature for ≥30 minutes before use.
• Use disposable pipette tips throughout the assay procedure to prevent cross-contamination.
• Unused strips should be resealed immediately and stored at 4 °C.
• Thorough mixing is crucial for reliable results; use a low-speed shaker or manually shake gently every 10 minutes.
• It is recommended to test samples and standards in duplicate or triplicate to improve data reliability.

Storage & Shipping

• Unopened Kit: Store protected from light at 2-8 °C. Shelf life is 24 months.
• After Opening: Please refer to the instructions for storage conditions of individual components.
• Shipping Conditions: Shipped with ice packs (blue ice) via cold chain to ensure consistent low temperature during transit.

Specifications & Pricing

Specification	Unit Price (¥)	Delivery Period
48 T	1,598.00	Please Inquire
96 T	2,558.00	Stock (Ships within 5 days)

Compliance Statement

This product is for research use only. Not intended for human or clinical diagnostic use. The user assumes full responsibility for any patent or licensing activities associated with the use of this kit. The supplier is not liable for any infringement or non-compliance issues.

Technical Background

Granzyme B is a key serine protease found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It works in conjunction with perforin to induce apoptosis in target cells. Recent studies indicate that non-cytotoxic cells (such as basophils, mast cells, and smooth muscle cells) can also produce Granzyme B, which is involved in the release of inflammatory mediators and extracellular matrix remodeling. Elevated levels of Granzyme B are closely associated with various pathological conditions, including autoimmune diseases, dermatological conditions, and type 1 diabetes, making it an important biomarker in immune and inflammation research. This ELISA kit employs a specific sandwich antibody pair to achieve accurate quantification of Granzyme B in mouse samples.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.