小鼠可溶性血管内皮细胞粘附分子1 ELISA定量试剂盒(VC

产品概述

产品名称：小鼠可溶性血管内皮细胞粘附分子1 ELISA定量试剂盒 (VCAM-1/CD106)

货号：KTE3126-1

本试剂盒是一款基于经典双抗体夹心法原理开发的高品质EliKine™ ELISA检测试剂盒，专为精准定量小鼠来源的细胞培养上清、血清或血浆样本中的可溶性VCAM-1（sVCAM-1/CD106）水平而设计。该产品凭借其优异的性能，成为心血管疾病、免疫炎症反应等研究领域中进行细胞因子定量分析的可靠工具。

核心特性与优势

• 高灵敏度与宽线性范围：检测下限低至7.81 pg/mL，检测范围覆盖7.81 pg/mL 至 500 pg/mL，确保对低丰度样本的准确检测和高浓度样本的精确稀释测定。
• 卓越的特异性：经过严格验证，对目标蛋白的类似物未观察到明显的交叉反应或背景干扰，保证了检测结果的可靠性。
• 优化的试剂组分：提供即用型或便捷配制形式的全套试剂，包括预包被微孔板、冻干标准品、检测抗体、链霉亲和素-HRP偶联物及完整的显色与洗涤系统，简化实验流程。
• 出色的重复性：建议进行复孔检测，有助于获得稳定、可重复的实验数据。

产品背景信息

血管细胞粘附分子-1 (VCAM-1/CD106) 是一种重要的细胞黏附分子，在介导白细胞（如淋巴细胞、单核细胞）与血管内皮细胞的黏附过程中发挥核心作用，参与动脉粥样硬化、类风湿性关节炎等多种病理过程。监测其可溶性形式（sVCAM-1）的水平，对于揭示相关疾病的发病机制具有重要的科研价值。

试剂盒组分（详细列表）

• Mouse sVCAM-1 预包被微孔板 (Mouse sVCAM-1 Microplate)
• Mouse sVCAM-1 标准品 (冻干) (Mouse sVCAM-1 Standard, lyophilized)
• 测定缓冲液 (10×浓缩) (Assay Buffer, 10×)
• Mouse sVCAM-1 检测抗体 (100×浓缩) (Mouse sVCAM-1 Detect Antibody, 100×)
• 链霉亲和素-HRP (100×浓缩) (Streptavidin-HRP, 100×)
• HRP 底物 (TMB) (HRP Substrate, TMB)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×浓缩) (Wash Buffer, 20×)
• 板盖 (Plate Covers)

技术参数

反应种属	小鼠
检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆
灵敏度	7.81 pg/mL
检测范围	7.81 - 500 pg/mL
产品规格	48 T / 96 T

保存与运输

保存建议：未开封的试剂盒应避光保存于2-8℃，保质期为24个月。启用后，请参照说明书妥善保存各组分。

运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿将不同批次的试剂或过期试剂混合使用。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条应立即放回原包装袋中密封，并置于4℃保存。
• 充分的混匀至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本产品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。

订购信息

规格	价格 (元)	货期
48 T	1598.0	请咨询
96 T	2558.0	期货（预计5个工作日内发货）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Mouse Soluble Vascular Cell Adhesion Molecule 1 ELISA Quantification Kit (VCAM-1/CD106)

Catalog Number: KTE3126-1

This kit is a high-quality EliKine™ ELISA detection kit developed based on the classic double-antibody sandwich principle, specifically designed for the accurate quantification of soluble VCAM-1 (sVCAM-1/CD106) levels in mouse-derived cell culture supernatants, serum, or plasma samples. With its excellent performance, this product serves as a reliable tool for cytokine quantitative analysis in research areas such as cardiovascular diseases and immune-inflammatory responses.

Core Features & Advantages

• High Sensitivity & Wide Linear Range: Lower limit of detection as low as 7.81 pg/mL, with a detection range covering 7.81 pg/mL to 500 pg/mL, ensuring accurate detection of low-abundance samples and precise dilution measurement of high-concentration samples.
• Excellent Specificity: Rigorously validated, showing no significant cross-reactivity or background interference with analogs of the target protein, guaranteeing reliable detection results.
• Optimized Reagent Components: Provides a complete set of reagents in ready-to-use or convenient-to-prepare forms, including pre-coated microplate, lyophilized standard, detection antibody, streptavidin-HRP conjugate, and a complete chromogenic and washing system, simplifying the experimental procedure.
• Outstanding Repeatability: Replicate testing is recommended to help obtain stable and reproducible experimental data.

Product Background Information

Vascular Cell Adhesion Molecule-1 (VCAM-1/CD106) is an important cell adhesion molecule that plays a central role in mediating the adhesion of leukocytes (such as lymphocytes and monocytes) to vascular endothelial cells, and is involved in various pathological processes such as atherosclerosis and rheumatoid arthritis. Monitoring the levels of its soluble form (sVCAM-1) holds significant research value for elucidating the pathogenesis of related diseases.

Kit Components (Detailed List)

• Mouse sVCAM-1 Pre-coated Microplate
• Mouse sVCAM-1 Standard (lyophilized)
• Assay Buffer (10× Concentrate)
• Mouse sVCAM-1 Detection Antibody (100× Concentrate)
• Streptavidin-HRP (100× Concentrate)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20× Concentrate)
• Plate Covers

Technical Parameters

Reactivity	Mouse
Detection Method	Sandwich ELISA
Sample Type	Cell Culture Supernatant, Serum, Plasma
Sensitivity	7.81 pg/mL
Detection Range	7.81 - 500 pg/mL
Product Size	48 T / 96 T

Storage & Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2-8℃, with a shelf life of 24 months. After opening, please store components properly according to the instructions.

Shipping Conditions: Shipped entirely under low-temperature conditions using ice packs (blue ice) to ensure reagent activity.

Important Notes

• Do not mix reagents from different batches or expired reagents.
• Before use, all reagents should be equilibrated at room temperature for approximately 30 minutes.
• To avoid cross-contamination, always use disposable pipette tips during the experiment.
• Unused strips should be immediately returned to the original foil pouch, sealed, and stored at 4°C.
• Adequate mixing is crucial; using a low-speed shaker or periodic manual shaking is recommended.
• For more reliable data, replicate testing (duplicate or triplicate wells) of samples and standards is recommended.

Warning: This product is for research use only, not for human use or clinical diagnosis. Users should ensure their application does not violate any patents or licensing regulations.

Ordering Information

Size	Price (CNY)	Lead Time
48 T	1598.0	Please Inquire
96 T	2558.0	Future Goods (Expected to ship within 5 business days)

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.