IL-18 ELISA KIT 白介素18ELISA试剂盒(

产品概述

货号：KTE3028-2 | 产品名称：Mouse IL-18 ELISA Kit (小鼠白介素18 ELISA试剂盒)

本试剂盒为EliKine™系列核心产品，专为科研领域中小鼠白介素18（IL-18）的高灵敏度定量检测而设计。采用成熟的夹心法ELISA原理，适用于细胞培养上清、血清及血浆等多种样本类型，确保实验结果稳定可靠。

主要特性与优势

• 高灵敏度：最低检测浓度达62.5 pg/mL，精准捕捉低丰度目标蛋白。
• 优异特异性：经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 宽线性范围：检测范围为62.5 pg/mL - 4,000 pg/mL，满足多样本浓度需求。
• 即用型组分：预包被微孔板与优化试剂配方，简化操作流程，缩短实验时间。

检测原理

本试剂盒基于双抗体夹心法原理。预先包被于微孔板上的特异性抗体捕获样本中的小鼠IL-18，洗涤后加入HRP标记的检测抗体形成复合物，经TMB底物显色反应，在450 nm波长下测定吸光度值，通过标准曲线精确计算样品中IL-18的浓度。

试剂盒组分

• 预包被Mouse IL-18微孔板
• Mouse IL-18标准品（冻干）
• 标准品稀释液
• 10×Assay Buffer
• 100×HRP标记检测抗体
• TMB显色底物
• 终止液
• 20×洗涤缓冲液
• 板盖

样本类型与适用性

适用于小鼠来源的细胞培养上清、血清及血浆样本。建议对样品和标准品进行复孔检测以提高结果准确性。

操作注意事项

• 请勿混合使用不同批号或过期试剂。
• 使用前请将所有组分室温平衡30分钟，确保试剂充分混匀。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 未使用的板条需立即密封，4℃保存。

保存与运输

未开封试剂盒避光保存于2-8℃，有效期24个月。开启后请参照说明书分别保存各组分。本品采用蓝冰冰袋运输，确保试剂活性。

产品规格与价格

规格	价格（元）	货期
48 T	1598.0	期货（5天内发货）
96 T	2558.0	期货（5天内发货）

背景信息

白细胞介素18（IL-18）是一种重要的促炎细胞因子，属于IL-1超家族，主要由巨噬细胞等免疫细胞分泌。它能够诱导NK细胞和T细胞产生干扰素γ，进而激活免疫应答，参与炎症反应及神经退行性疾病等病理过程。

警告：本品仅用于科学研究，不适用于人类或临床诊断。使用前请仔细阅读产品说明书，并遵守相关实验室安全规范。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog Number: KTE3028-2 | Product Name: Mouse IL-18 ELISA Kit

This kit is a core product of the EliKine™ series, specifically designed for the highly sensitive quantitative detection of Mouse Interleukin-18 (IL-18) in research fields. It utilizes a mature sandwich ELISA principle and is suitable for various sample types including cell culture supernatants, serum, and plasma, ensuring stable and reliable experimental results.

Key Features & Advantages

• High Sensitivity: Minimum detection concentration reaches 62.5 pg/mL, accurately capturing low-abundance target proteins.
• Excellent Specificity: Rigorously validated, showing no significant cross-reactivity or background interference with homologous proteins.
• Wide Linear Range: Detection range from 62.5 pg/mL to 4,000 pg/mL, meeting the needs of samples with varying concentrations.
• Ready-to-Use Components: Pre-coated microplate and optimized reagent formulations simplify the operation process and reduce experimental time.

Detection Principle

This kit is based on a double-antibody sandwich principle. The specific antibody pre-coated on the microplate captures Mouse IL-18 from the sample. After washing, an HRP-labeled detection antibody is added to form a complex. A colorimetric reaction is developed using TMB substrate, and the absorbance is measured at 450 nm. The concentration of IL-18 in the sample is precisely calculated from a standard curve.

Kit Components

• Pre-coated Mouse IL-18 Microplate
• Mouse IL-18 Standard (Lyophilized)
• Standard Diluent
• 10× Assay Buffer
• 100× HRP-labeled Detection Antibody
• TMB Substrate
• Stop Solution
• 20× Wash Buffer
• Plate Seal

Sample Types & Applicability

Suitable for mouse-derived cell culture supernatants, serum, and plasma samples. It is recommended to perform duplicate tests for both samples and standards to improve result accuracy.

Operation Precautions

• Do not mix reagents from different lots or use expired reagents.
• Before use, equilibrate all components to room temperature for 30 minutes and ensure thorough mixing.
• Use disposable pipette tips during the experiment to avoid cross-contamination.
• Unused strips should be sealed immediately and stored at 4°C.

Storage & Shipping

Store the unopened kit protected from light at 2-8°C; it is stable for 24 months. After opening, store individual components as described in the instructions. This product is shipped with blue ice packs to ensure reagent activity.

Specifications & Pricing

Specification	Price (USD)	Availability
48 T	1598.0	Futures (Ships within 5 days)
96 T	2558.0	Futures (Ships within 5 days)

Background Information

Interleukin-18 (IL-18) is an important pro-inflammatory cytokine belonging to the IL-1 superfamily. It is primarily secreted by immune cells such as macrophages. It can induce NK cells and T cells to produce interferon-gamma, thereby activating immune responses and participating in pathological processes such as inflammatory reactions and neurodegenerative diseases.

Warning: This product is intended for research use only and is not suitable for human use or clinical diagnosis. Please read the product instructions carefully before use and adhere to relevant laboratory safety regulations.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeen Company.