IL-13 ELISA KIT 白介素13ELISA试剂盒(

小鼠IL-13 ELISA Kit (白介素13ELISA试剂盒)

货号：KTE3026-2 | 产品分类：核心产品 - EliKine™系列

产品概述

本试剂盒是一款基于经典双抗体夹心法原理开发的高品质ELISA检测产品，专门用于精准定量小鼠来源的样本（如细胞培养上清、血清或血浆）中的白介素13 (IL-13) 含量。作为细胞分析领域的核心工具，其设计旨在为免疫学、炎症机制及传染病研究提供可靠的数据支持。

核心特性与优势

• 高灵敏度与特异性：最低检测浓度达到15.63 pg/mL，检测范围覆盖15.63 pg/mL - 1,000 pg/mL。经严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰，确保数据的准确性。
• 优化的试剂组分：试剂盒提供完整且稳定的组分，包括预包被微孔板、冻干标准品、检测抗体、链霉亲和素-HRP偶联物及全套缓冲液，方便实验操作。
• 可靠的检测性能：采用经过验证的配对抗体，确保检测结果的高重复性和线性关系。

技术参数

反应性	小鼠 (Mouse)	检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆	灵敏度	15.63 pg/mL
检测范围	15.63 - 1000 pg/mL	保存条件	未开封试剂盒避光保存于2-8°C

产品背景

白细胞介素13 (IL-13) 是一种主要由Th2细胞分泌的多效性细胞因子，在过敏性炎症、哮喘、抗寄生虫免疫以及调节巨噬细胞中HIV-1病毒复制等生理和病理过程中扮演关键角色。其基因位于染色体5q31，与IL-4具有同源性。本试剂盒通过特异性捕获并检测小鼠IL-13，助力于相关疾病模型的深入研究。

试剂盒组分清单

• Mouse IL-13 Pre-coated Microplate (96-well or 48-well format)
• Mouse IL-13 Standard (lyophilized)
• Standard Diluent Buffer
• 10× Assay Buffer
• Mouse IL-13 Biotinylated Detection Antibody (100×)
• Streptavidin-HRP Conjugate (100×)
• TMB Substrate Solution
• Stop Solution
• 20× Wash Buffer
• Plate Covers

规格与价格

规格	价格 (人民币)	货期
48 T	1598.0	期货（预计5个工作日内发货）
96 T	2558.0	期货（预计5个工作日内发货）

重要操作提示

• 请勿混合使用不同批号的试剂或已过有效期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为获得最佳结果，建议对样品和标准品进行复孔检测（双孔或三孔）。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 未使用的板条应立即放回原包装袋并密封，于4°C保存。
• 充分的混匀步骤对实验结果至关重要。

运输与储存

运输：全程采用蓝冰（冰袋）低温运输，以确保试剂稳定性。
储存：未开封试剂盒请避光保存于2-8°C，有效期24个月。启用后，请参照说明书分别保存各组分。

警告： 本产品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-13 ELISA Kit

Catalog Number: KTE3026-2 | Category: Core Products - EliKine™ Series

Product Overview

This kit is a high-quality ELISA detection product developed based on the classic double-antibody sandwich principle, specifically designed for the accurate quantification of Interleukin-13 (IL-13) levels in mouse-derived samples (such as cell culture supernatants, serum, or plasma). As a core tool in the field of cell analysis, it is designed to provide reliable data support for immunology, inflammation mechanism, and infectious disease research.

Key Features & Advantages

• High Sensitivity & Specificity: Minimum detectable concentration reaches 15.63 pg/mL, with a detection range of 15.63 pg/mL - 1,000 pg/mL. Rigorously validated to show no significant cross-reactivity or background interference with analogs of the target protein, ensuring data accuracy.
• Optimized Reagent Components: The kit provides complete and stable components, including pre-coated microplates, lyophilized standards, detection antibodies, Streptavidin-HRP conjugates, and a full set of buffers for convenient experimental operation.
• Reliable Detection Performance: Utilizes validated antibody pairs to ensure high repeatability and linearity of detection results.

Technical Parameters

Reactivity	Mouse	Detection Method	Sandwich ELISA
Sample Type	Cell Culture Supernatant, Serum, Plasma	Sensitivity	15.63 pg/mL
Detection Range	15.63 - 1000 pg/mL	Storage Conditions	Unopened kit should be stored protected from light at 2-8°C

Background

Interleukin-13 (IL-13) is a pleiotropic cytokine primarily secreted by Th2 cells, playing a key role in physiological and pathological processes such as allergic inflammation, asthma, anti-parasitic immunity, and regulating HIV-1 virus replication in macrophages. Its gene is located on chromosome 5q31 and shares homology with IL-4. This kit facilitates in-depth research on related disease models by specifically capturing and detecting mouse IL-13.

Kit Components

• Mouse IL-13 Pre-coated Microplate (96-well or 48-well format)
• Mouse IL-13 Standard (lyophilized)
• Standard Diluent Buffer
• 10× Assay Buffer
• Mouse IL-13 Biotinylated Detection Antibody (100×)
• Streptavidin-HRP Conjugate (100×)
• TMB Substrate Solution
• Stop Solution
• 20× Wash Buffer
• Plate Covers

Specifications & Pricing

Specification	Price (RMB)	Lead Time
48 T	1598.0	Future goods (Estimated shipment within 5 business days)
96 T	2558.0	Future goods (Estimated shipment within 5 business days)

Important Operational Notes

• Do not mix reagents from different lot numbers or use expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• For optimal results, it is recommended to perform replicate testing (duplicate or triplicate wells) for both samples and standards.
• Use disposable pipette tips during the experiment to avoid cross-contamination.
• Unused strips should be immediately returned to the original packaging bag, sealed, and stored at 4°C.
• Adequate mixing steps are crucial for experimental results.

Shipping & Storage

Shipping: Shipped with blue ice (ice packs) throughout to ensure reagent stability.
Storage: Unopened kits should be stored protected from light at 2-8°C, valid for 24 months. After opening, please store individual components separately according to the instructions.

Warning: This product is for research use only, not for human or clinical diagnostic use. Users should ensure that its application does not infringe upon any patents or licensing regulations.

Note: The above information may change due to factors such as product optimization and upgrades. For specific information, please consult ArKayBio.