小鼠免疫球蛋白同型鉴定 ELISA定量试剂盒(IgA/IgM

小鼠免疫球蛋白同型鉴定 ELISA定量试剂盒 (货号: KTE3140-1)

本产品是一款基于夹心法ELISA原理的高品质检测试剂盒，专为定性测定杂交瘤细胞培养上清液、腹水以及纯化抗体样本中的小鼠免疫球蛋白亚型而设计。它可同时鉴定6种重链亚型（IgA, IgM, IgG1, IgG2a, IgG2b, IgG3）和2种轻链亚型（κ链, λ链），是杂交瘤技术研究与单克隆抗体开发领域的核心工具。

产品详情

• 产品英文名称: Mouse Ig Isotyping ELISA Kit
• 产品分类: 核心产品 - 细胞分析
• 反应性: 特异性针对小鼠样本
• 检测方法: 夹心法ELISA

主要特点与优势

• 全面覆盖: 一次实验即可完成8种关键免疫球蛋白亚型的鉴定，为抗体分型提供全面数据支持。
• 高特异性: 采用EliKine™技术，经过严格验证，对目标蛋白的类似物未发现明显的交叉反应或背景干扰，结果准确可靠。
• 操作便捷: 试剂盒提供优化好的预包被微孔板与即用型试剂，大大简化了实验流程，节省准备时间。
• 实用指导: 准确的亚型鉴定结果，对于后续选择最合适的抗体纯化方案（如蛋白A/G/L亲和层析）具有重要的指导价值。

试剂盒组分

试剂盒包含进行实验所需的全部核心组分：

• Mouse Ig Isotyping Microplate (预包被微孔板)
• Positive Control (阳性对照)
• Assay Buffer (10×) (检测缓冲液)
• HRP-conjugated Mouse Ig Isotyping Detect Antibody (100×) (HRP标记检测抗体)
• HRP Substrate (TMB) (HRP底物)
• Stop Solution (终止液)
• Wash Buffer (20×) (洗涤缓冲液)
• Plate Covers (板盖)

应用背景

在单克隆抗体制备过程中，对抗体进行准确的同型鉴定是至关重要的一步。不同亚型的免疫球蛋白在生物化学特性（如分子量、与蛋白A/G/L的结合能力）上存在差异，这会直接影响下游纯化方法的选择与效率。本试剂盒是区分和鉴定每个潜在杂交瘤克隆的高效工具，有助于研究人员快速筛选出目标单克隆抗体。

技术规格与订购信息

保存与运输

• 保存建议: 未启封的试剂盒请避光保存于2-8℃，保质期24个月。启用后，请参考说明书分别保存各组分。
• 运输条件: 全程冰袋（蓝冰）运输，确保试剂活性。

重要注意事项

• 请勿将不同批号或已过有效期的试剂混合使用。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 建议对样品和标准品进行复孔检测（双孔或三孔），以确保结果的准确性。
• 充分混匀试剂对实验结果至关重要，推荐使用振荡器或定期手动摇匀。

警告: 本产品仅用于科学研究，不适用于人类或临床诊断。使用前请务必阅读产品说明书。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Immunoglobulin Isotyping ELISA Quantitative Kit (Cat#: KTE3140-1)

This product is a high-quality detection kit based on the sandwich ELISA principle, specifically designed for the qualitative determination of mouse immunoglobulin subclasses in hybridoma cell culture supernatants, ascites, and purified antibody samples. It can simultaneously identify 6 heavy chain subclasses (IgA, IgM, IgG1, IgG2a, IgG2b, IgG3) and 2 light chain types (κ-chain, λ-chain), serving as a core tool in hybridoma technology research and monoclonal antibody development.

Product Details

• Product English Name: Mouse Ig Isotyping ELISA Kit
• Product Category: Core Products - Cell Analysis
• Reactivity: Specific for mouse samples
• Detection Method: Sandwich ELISA

Key Features & Advantages

• Comprehensive Coverage: Enables identification of 8 key immunoglobulin subclasses in a single experiment, providing comprehensive data support for antibody isotyping.
• High Specificity: Utilizes EliKine™ technology, rigorously validated to show no significant cross-reactivity or background interference with analogs of the target protein, ensuring accurate and reliable results.
• User-Friendly Operation: The kit provides optimized pre-coated microplates and ready-to-use reagents, significantly simplifying the experimental procedure and saving preparation time.
• Practical Guidance: Accurate isotyping results are highly valuable for guiding the subsequent selection of the most appropriate antibody purification strategy (e.g., Protein A/G/L affinity chromatography).

Kit Components

The kit contains all core components required for the experiment:

• Mouse Ig Isotyping Microplate
• Positive Control
• Assay Buffer (10×)
• HRP-conjugated Mouse Ig Isotyping Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Application Background

Accurate antibody isotyping is a crucial step in the monoclonal antibody preparation process. Immunoglobulins of different subclasses exhibit differences in biochemical properties (such as molecular weight and binding capacity to Protein A/G/L), which directly impact the selection and efficiency of downstream purification methods. This kit is a high-efficiency tool for differentiating and identifying each potential hybridoma clone, aiding researchers in rapidly screening for target monoclonal antibodies.

Technical Specifications & Ordering Information

Storage & Shipping

• Storage Recommendation: Store the unopened kit protected from light at 2-8°C. The shelf life is 24 months. After opening, please refer to the manual for separate storage of components.
• Shipping Conditions: Shipped with ice packs (blue ice) throughout to ensure reagent activity.

Important Notes

• Do not mix reagents from different lot numbers or expired reagents.
• All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
• To avoid cross-contamination, always use disposable pipette tips during the experiment.
• Replicate testing (duplicate or triplicate wells) for samples and standards is recommended to ensure result accuracy.
• Thorough mixing of reagents is crucial for experimental results; the use of a vortex mixer or regular manual shaking is recommended.

Warning: This product is for research use only, not intended for human or clinical diagnostic use. Please read the product manual carefully before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.