- ￥1598 - 2558
- 亚科因(Abbkine)
- 31.25 pg/mL-2,000 pg/mL
- 31.25 pg/mL
- 湖北武汉
- KTE3012-2
- 2026年01月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
亚科因(Abbkine)
- 检测范围：
31.25 pg/mL-2,000 pg/mL
- 检测方法：
夹心法
- 应用：
ELISA检测
- 适应物种：
小鼠
- 样本：
细胞培养上清#血清#血浆
- 灵敏度：
31.25 pg/mL
- 规格：
48 T/96 T
| 规格： | 48 T | 产品价格： | ￥1598.0 |
|---|---|---|---|
| 规格： | 96 T | 产品价格： | ￥2558.0 |
产品概述
EliKine™ Mouse CXCL1/KC ELISA Kit是一款专为小鼠样本设计的高灵敏度、高特异性酶联免疫吸附检测试剂盒，适用于细胞培养上清、血清及血浆样本的定量分析。产品采用双抗体夹心法，检测范围覆盖31.25 pg/mL至2,000 pg/mL，最低检测灵敏度达31.25 pg/mL，可精准量化小鼠趋化因子CXCL1的表达水平。
核心特性
- 高特异性：对目标蛋白类似物无显著交叉反应，背景干扰低。
- 宽检测范围：覆盖生理及病理状态下CXCL1的典型浓度区间。
- 即用型组分：预包被微孔板、冻干标准品、检测抗体及HRP底物等齐全试剂，开盒即用。
- 复孔推荐：建议对样品和标准品进行双孔或三孔检测，确保数据可靠性。
试剂盒组分
| 组分名称 | 规格 | 保存条件 |
|---|---|---|
| Mouse CXCL1 Microplate | 1板 | 2-8℃ |
| Mouse CXCL1 Standard (冻干) | 2支 | -20℃ |
| Standard Diluent | 1瓶 | 2-8℃ |
| Assay Buffer (10×) | 1瓶 | 2-8℃ |
| Mouse CXCL1 Detect Antibody (100×) | 1瓶 | 2-8℃ |
| Streptavidin-HRP (100×) | 1瓶 | 2-8℃ |
| HRP Substrate (TMB) | 1瓶 | 2-8℃（避光） |
| Stop Solution | 1瓶 | 2-8℃ |
| Wash Buffer (20×) | 1瓶 | 2-8℃ |
| Plate Covers | 3片 | 室温 |
技术参数
- 产品货号：KTE3012-2
- 检测方法：夹心法ELISA
- 反应性：仅限小鼠
- 样本类型：细胞培养上清、血清、血浆
- 规格选项：48T（货期咨询）/ 96T（货期咨询）
保存与运输
未开封试剂盒需避光保存于2-8℃，保质期24个月；开封后请按组分说明分别保存。运输条件为冰袋（蓝冰）冷链，确保试剂活性稳定。
应用背景
小鼠CXCL1（趋化因子C-X-C motif配体1）是一种参与炎症调节、血管生成及肿瘤发展的关键细胞因子。通过与其受体CXCR2结合，CXCL1在中性粒细胞趋化、脊髓发育及神经保护中发挥重要作用。本试剂盒为研究小鼠模型中的免疫应答、神经炎症及相关疾病机制提供可靠工具。
注意事项
- 请勿混用不同批号试剂或超期试剂。
- 使用前需室温平衡30分钟，避免剧烈震荡。
- 实验过程中建议使用一次性吸头，防止交叉污染。
- 未使用的板条需立即密封并冷藏保存。
注：本品仅供科研使用，不适用于临床诊断。
```注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。
Product Overview
The EliKine™ Mouse CXCL1/KC ELISA Kit is a high-sensitivity, high-specificity enzyme-linked immunosorbent assay kit specifically designed for mouse samples, suitable for the quantitative analysis of cell culture supernatants, serum, and plasma samples. The kit employs a double-antibody sandwich method, with a detection range covering 31.25 pg/mL to 2,000 pg/mL and a minimum detection sensitivity of 31.25 pg/mL, enabling precise quantification of mouse chemokine CXCL1 expression levels.
Key Features
- High Specificity: No significant cross-reactivity with target protein analogs, low background interference.
- Wide Detection Range: Covers the typical concentration range of CXCL1 under physiological and pathological conditions.
- Ready-to-Use Components: Complete reagents including pre-coated microplate, lyophilized standard, detection antibody, and HRP substrate, ready for immediate use.
- Recommended Duplicate/Triplicate Wells: It is advised to perform duplicate or triplicate tests for samples and standards to ensure data reliability.
Kit Components
| Component Name | Specification | Storage Condition |
|---|---|---|
| Mouse CXCL1 Microplate | 1 plate | 2-8℃ |
| Mouse CXCL1 Standard (Lyophilized) | 2 vials | -20℃ |
| Standard Diluent | 1 bottle | 2-8℃ |
| Assay Buffer (10×) | 1 bottle | 2-8℃ |
| Mouse CXCL1 Detect Antibody (100×) | 1 bottle | 2-8℃ |
| Streptavidin-HRP (100×) | 1 bottle | 2-8℃ |
| HRP Substrate (TMB) | 1 bottle | 2-8℃ (Protect from light) |
| Stop Solution | 1 bottle | 2-8℃ |
| Wash Buffer (20×) | 1 bottle | 2-8℃ |
| Plate Covers | 3 sheets | Room Temperature |
Technical Parameters
- Product Catalog Number: KTE3012-2
- Detection Method: Sandwich ELISA
- Reactivity: Mouse specific
- Sample Types: Cell culture supernatant, serum, plasma
- Specification Options: 48T (Lead time inquiry) / 96T (Lead time inquiry)
Storage and Shipping
Unopened kits should be stored protected from light at 2-8℃ with a shelf life of 24 months; after opening, please store components separately according to the instructions. Shipping conditions involve cool pack (blue ice) cold chain transport to ensure reagent stability.
Application Background
Mouse CXCL1 (C-X-C motif chemokine ligand 1) is a key cytokine involved in inflammation regulation, angiogenesis, and tumor development. By binding to its receptor CXCR2, CXCL1 plays important roles in neutrophil chemotaxis, spinal cord development, and neuroprotection. This kit provides a reliable tool for studying immune responses, neuroinflammation, and related disease mechanisms in mouse models.
Precautions
- Do not mix reagents from different lot numbers or use expired reagents.
- Allow reagents to equilibrate to room temperature for 30 minutes before use, avoid vigorous shaking.
- It is recommended to use disposable pipette tips during the experiment to prevent cross-contamination.
- Unused strips should be sealed immediately and stored refrigerated.
Note: This product is intended for research use only and is not suitable for clinical diagnosis.
Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.
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