小鼠白介素-10 ELISA定量试剂盒(IL-10)

Mouse IL-10 ELISA Kit (KTE7010-1)

产品概览

本套件为 ELISA 夹心法检测平台，专为小鼠来源的白介素-10 (IL‑10) 定量分析设计，适用于细胞培养上清、血清、血浆以及其他生物液体样本。产品归属 细胞分析 中心的 核心产品，广泛服务于蛋白类 ELISA应用。

技术原理

套装采用双抗体夹心技术：

1. 微孔板预包被小鼠特异性 IL‑10 捕获抗体。
2. 样品中 IL‑10 与捕获抗体形成复合物。
3. 加入生物素化检测抗体后形成三层复合物。
4. EliKine™ Streptavidin‑HRP 与检测抗体结合，随后通过 HRP 底物产生可测光的信号。
5. 使用反应终止液固定颜色，按标准曲线计算样本中 IL‑10 含量。

主要特点 & 优势

• 极高灵敏度：检测下限 50 pg/mL，检测范围 93.75 pg/mL – 6000 pg/mL。
• 特异性强：未检测到显著的交叉反应或背景干扰。
• 全部试剂均为小鼠源抗体，确保与小鼠样本的匹配度。
• 标准品提供双/三孔重复检测选项，提高实验 reproducibility。
• 配套的封板膜、洗涤缓冲液等完整组件，便于一次性完成整套实验。

试剂盒组成

• 小鼠源 IL‑10 抗体预包被微孔板
• 小鼠源 IL‑10 蛋白标准品
• 小鼠源 IL‑10 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

使用说明要点

• 不同批号或过期的试剂请勿混用。
• 使用前将所有试剂在室温平衡 30 分钟。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 未使用的板条请密封回包装，置 4 ℃保存。
• 混匀对结果影响显著，推荐低速振荡或每 10 分钟手动摇匀。
• 样品和标准品建议做双孔或三孔检测（复孔）。

存储与运输

项目	要求
未开封试剂盒	2 ℃–8 ℃保存
启用后试剂	请依据说明书各组分的保存条件操作
运输方式	冰袋（蓝冰）冷链运输

警示声明

本产品仅限用于科研用途，不适用于人类或临床诊断。请勿将本套件用于违反任何专利或许可证的行为，因使用本产品产生的专利侵权或其他违规后果，本公司概不负责。

产品规格 & 价格

规格	价格 (CNY)	货期
48 T	1598.0	现货（次日发货）
96 T	2558.0	现货（次日发货）

检索信息

基因 ID：16153
别名：IL10, CSIF, IL‑10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
蛋白质 ID：P18893
蛋白序列：UniProt 访问链接

适用领域

本套件适合免疫调节、炎症研究、细胞因子网络分析等方向的科研项目，帮助实验室快速、准确地评估小鼠模型中 IL‑10 的表达水平。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-10 ELISA Kit (KTE7010-1)

Product Overview

This kit is an ELISA sandwich assay platform specifically designed for quantitative analysis of mouse‑derived interleukin‑10 (IL‑10), suitable for cell culture supernatants, serum, plasma, and other biological fluid samples. The product is part of the Cell Analysis division’s core products, widely supporting protein ELISA applications.

Technical Principle

The kit employs a dual‑antibody sandwich format:

1. Microplate pre‑coated with mouse‑specific IL‑10 capture antibody.
2. IL‑10 in the sample forms a complex with the capture antibody.
3. Addition of biotinylated detection antibody creates a three‑layer complex.
4. EliKine™ Streptavidin‑HRP binds to the detection antibody, and HRP substrate generates a measurable optical signal.
5. Reaction stop solution fixes the color, and IL‑10 concentration is calculated from a standard curve.

Key Features & Advantages

• Ultra‑high sensitivity: detection limit 50 pg/mL; dynamic range 93.75 pg/mL – 6000 pg/mL.
• High specificity: no significant cross‑reactivity or background interference detected.
• All reagents are mouse‑derived antibodies, ensuring optimal compatibility with mouse samples.
• Standards are provided with duplicate or triplicate well options to enhance experimental reproducibility.
• Complete set of sealing film, wash buffer, etc., enables one‑step completion of the entire assay.

Kit Components

• Mouse‑derived IL‑10 antibody pre‑coated microplate
• Mouse‑derived IL‑10 protein standard
• Mouse‑derived IL‑10 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer
• Assay buffer
• HRP substrate
• Reaction stop solution
• Wash buffer
• Plate sealing film

Critical Usage Notes

• Do not mix reagents from different lot numbers or expired kits.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips during the assay to avoid cross‑contamination.
• Unspent plate strips should be resealed in their original packaging and stored at 4 °C.
• Mixing significantly influences results; gentle orbital shaking or manual inversion every 10 minutes is recommended.
• Samples and standards are recommended to be run in duplicate or triplicate wells (repeat wells).

Storage & Shipping

Item	Requirement
Unopened kit	Store at 2 °C–8 °C
Opened reagents	Follow the storage conditions specified for each component in the manual
Shipping method	Cold‑chain transport with ice packs (blue ice)

Warning Statement

This product is intended for research use only and is not suitable for human or clinical diagnostics. Do not use this kit in any activity that infringes patents or licenses; the company assumes no responsibility for any patent infringement or other violations arising from product use.

Product Specifications & Pricing

Specification	Price (CNY)	Lead Time
48 T	1598.0	In stock (next‑day shipment)
96 T	2558.0	In stock (next‑day shipment)

Reference Information

Gene ID: 16153
Aliases: IL10, CSIF, IL‑10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
Protein ID: P18893
Protein sequence: UniProt link

Applicable Fields

This kit is suitable for research projects focusing on immune modulation, inflammation studies, cytokine network analysis, and related areas, enabling laboratories to rapidly and accurately assess IL‑10 expression levels in mouse models.

Note: The information above may change due to product optimization or other factors; please contact Yakein Co. for the most current details.