HGF ELISA KIT 肝细胞生长因子ELISA试剂盒(

EliKine™ 小鼠肝细胞生长因子 (HGF) ELISA 试剂盒 (KTE3053-2)

本试剂盒是一款基于经典双抗体夹心法原理的高灵敏度定量检测试剂盒，专门设计用于精准检测小鼠来源样本（如细胞培养上清、血清、血浆）中的肝细胞生长因子 (HGF) 蛋白浓度。作为我们细胞分析产品线的核心产品，它以其卓越的性能为生命科学研究提供可靠的数据支持。

产品概述

中文名称：HGF ELISA KIT 肝细胞生长因子ELISA试剂盒(小鼠)
英文名称：Mouse HGF ELISA Kit
货号：KTE3053-2
反应性：小鼠
检测范围：0.16 ng/mL - 10 ng/mL
灵敏度：0.16 ng/mL
样本类型：细胞培养上清、血清、血浆

核心特点与优势

• 高灵敏度与特异性：EliKine™ 技术平台确保了极低的检测下限，能够精确检测低丰度 HGF 蛋白。经严格验证，对目标蛋白的类似物未观察到明显的交叉反应或背景干扰。
• 可靠的检测性能：优化的试剂配方和反应体系保证了检测结果的准确性和可重复性。
• 即用型完整组分：试剂盒提供实验所需的所有核心组分，包括预包被微孔板、冻干标准品、检测抗体、HRP 底物等，开盒即可使用，简化实验流程。
• 灵活的规格选择：提供 48 T 和 96 T 两种规格，满足不同通量的实验需求。

试剂盒组分

• Mouse HGF 预包被微孔板 (Mouse HGF Microplate)
• Mouse HGF 标准品 (冻干) (Mouse HGF Standard, lyophilized)
• 标准品稀释液 (Standard Diluent)
• 检测缓冲液 (10×) (Assay Buffer 10×)
• 小鼠 HGF 检测抗体 (100×) (Mouse HGF Detect Antibody 100×)
• 链霉亲和素-HRP (100×) (Streptavidin-HRP 100×)
• HRP 底物 (TMB) (HRP Substrate TMB)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×) (Wash Buffer 20×)
• 板覆膜 (Plate Covers)

背景信息

肝细胞生长因子 (HGF) 是一种由间质细胞分泌的重要多功能细胞因子，在细胞生长、运动、形态发生、血管生成、组织再生以及肿瘤发生等生理和病理过程中扮演着核心角色。它通过与其受体 c-Met 结合，激活下游酪氨酸激酶信号通路。对小鼠 HGF 水平的精确检测，在相关疾病模型的研究中具有重要价值。

技术参数

规格	货号	价格 (元)	货期
48 T	KTE3053-2-48T	1598.0	期货（预计5个工作日内发货）
96 T	KTE3053-2-96T	2558.0	期货（预计5个工作日内发货）

保存与运输

保存建议：未启封的试剂盒请避光保存于 2-8°C，有效期为 24 个月。各组分启封后，请参照说明书建议的条件妥善保存。
运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约 30 分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 每次检测后，请立即将未使用的板条放回原包装袋并密封，置于 4°C 保存。
• 实验过程中充分的混匀至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的结果，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本产品仅用于科学研究用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定，销售方对因使用本产品可能导致的专利侵权或其他违规行为不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Hepatocyte Growth Factor (HGF) ELISA Kit (KTE3053-2)

This kit is a highly sensitive quantitative detection kit based on the classic double-antibody sandwich principle, specifically designed for the accurate detection of Hepatocyte Growth Factor (HGF) protein concentration in mouse-derived samples (such as cell culture supernatant, serum, plasma). As a core product of our Cell Analysis product line, it provides reliable data support for life science research with its excellent performance.

Product Overview

Chinese Name: HGF ELISA KIT 肝细胞生长因子ELISA试剂盒(小鼠)
English Name: Mouse HGF ELISA Kit
Catalog Number: KTE3053-2
Reactivity: Mouse
Detection Range: 0.16 ng/mL - 10 ng/mL
Sensitivity: 0.16 ng/mL
Sample Types: Cell culture supernatant, serum, plasma

Core Features & Advantages

• High Sensitivity & Specificity: The EliKine™ technology platform ensures a very low detection limit, enabling precise detection of low-abundance HGF protein. Strict validation shows no significant cross-reactivity or background interference with analogs of the target protein.
• Reliable Detection Performance: Optimized reagent formulation and reaction system guarantee accurate and reproducible results.
• Ready-to-Use Complete Components: The kit provides all core components required for the experiment, including pre-coated microplate, lyophilized standard, detection antibody, HRP substrate, etc., ready for immediate use, simplifying the experimental workflow.
• Flexible Size Options: Available in 48 T and 96 T sizes to meet different throughput requirements.

Kit Components

• Mouse HGF Pre-coated Microplate
• Mouse HGF Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse HGF Detect Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Background Information

Hepatocyte Growth Factor (HGF) is an important multifunctional cytokine secreted by mesenchymal cells, playing a central role in physiological and pathological processes such as cell growth, motility, morphogenesis, angiogenesis, tissue regeneration, and tumorigenesis. It activates downstream tyrosine kinase signaling pathways by binding to its receptor, c-Met. Accurate detection of mouse HGF levels is of significant value in research involving related disease models.

Technical Parameters

Size	Catalog Number	Price (CNY)	Delivery Time
48 T	KTE3053-2-48T	1598.0	Future (Estimated shipment within 5 business days)
96 T	KTE3053-2-96T	2558.0	Future (Estimated shipment within 5 business days)

Storage & Shipping

Storage Recommendation: Store the unopened kit protected from light at 2-8°C; the shelf life is 24 months. After opening each component, store properly according to the instructions.
Shipping Conditions: Shipped with ice packs (blue ice) throughout to ensure reagent activity.

Important Notes

• Do not mix reagents from different lot numbers or expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• To avoid cross-contamination, use disposable pipette tips during the experiment.
• After each assay, immediately return unused strips to the original foil pouch and seal, storing at 4°C.
• Thorough mixing during the experiment is crucial; use a low-speed shaker or periodic manual shaking is recommended.
• For more reliable results, duplicate or triplicate wells for samples and standards are recommended.

Warning: This product is for research use only, not intended for human or clinical diagnostic use. The user should ensure that its application does not violate any patent or license agreements. The seller assumes no responsibility for any patent infringement or other violations that may result from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.