小鼠干细胞因子 ELISA定量试剂盒(SCF)

小鼠干细胞因子（SCF）ELISA定量试剂盒 KTE3133-1

产品概述

本套件为EliKine™系列核心产品，专为细胞分析实验室设计，采用双抗体夹心ELISA技术实现小鼠干细胞因子（SCF）的高灵敏度定量检测。适用于细胞培养上清、血清及血浆等样本，广泛服务于蛋白类研究及ELISA应用场景。

关键特性与优势

• 极高灵敏度：检测下限 7.81 pg/mL。
• 宽广检测范围：7.81 pg/mL – 500 pg/mL。
• 特异性强，未检测到显著交叉反应或背景干扰。
• 采用 100× 检测抗体与 100× 链霉亲和素‑HRP，保证信号放大。
• 完整的试剂包涵 48 孔或 96 孔微孔板，满足不同通量需求。

试剂盒组成

• Mouse SCF Microplate
• Mouse SCF Standard (lyophilized)
• Assay Buffer (10×)
• Mouse SCF Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

检测原理

本试剂盒基于双抗体夹心法：

1. 预包被的抗小鼠SCF抗体捕获样品或标准品中的SCF。
2. 洗涤去除未结合组分。
3. 加入HRP标记的检测抗体形成二抗复合物。
4. 再次洗涤后加入TMB底物，HRP催化产生蓝色显色。
5. 加入终止液后颜色转为黄色，在450 nm波长读取OD值。

适用样本类型

• 细胞培养上清
• 血清
• 血浆

性能指标

检测范围	7.81 pg/mL – 500 pg/mL
灵敏度	7.81 pg/mL
反应性物种	小鼠
检测方法	夹心ELISA

使用注意事项

• 不同批号或已过期的试剂不得混用。
• 使用前请将试剂在室温平衡30 分钟。
• 实验操作建议使用一次性吸头，防止交叉污染。
• 未使用的板条请放回包装，密闭保存于4 ℃。
• 混匀对结果影响显著，推荐低速振荡或每10 分钟手动轻摇。
• 样品及标准建议设置双孔或三孔（复孔）以提高可靠性。

存储与运输

• 未开封试剂盒请避光存放于2‑8 ℃，保质期 24 个月。
• 使用后各组分请参照说明书具体保存。
• 运输方式：冰袋（蓝冰）冷链运输，确保试剂活性。

背景信息

干细胞因子（SCF）是能够与c‑Kit受体结合的细胞因子，既以跨膜形式亦以可溶形式存在。SCF在肥大细胞粘附、迁移、增殖及存活中发挥关键作用，并参与组胺、类胰蛋白酶的释放，影响过敏应答。此外，SCF与IL‑3、IL‑6、IL‑11、GM‑CSF、G‑CSF、EPO等多种生长因子协同，诱导髓系和红系集落形成，涉及造血、精子发生、黑素生成及心肌修复等生理过程。

规格与价格

规格	单价（人民币）	货期
48 T	1,598.00	请咨询
96 T	2,558.00	期货（5 天内发货）

重要声明

本产品仅供科研使用，不适用于人类或临床诊断。本公司不对因使用本产品而可能产生的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Stem Cell Factor (SCF) ELISA Quantitation Kit KTE3133-1

Product Overview

This kit is a core product of the EliKine™ series, specifically designed for cellular analysis laboratories. It utilizes a double‑antibody sandwich ELISA platform to achieve high‑sensitivity quantification of mouse Stem Cell Factor (SCF). The kit is compatible with cell culture supernatants, serum, and plasma samples, and broadly serves protein research and ELISA applications.

Key Features and Advantages

• Ultra‑high sensitivity: limit of detection 7.81 pg/mL.
• Broad detection range: 7.81 pg/mL – 500 pg/mL.
• High specificity with no significant cross‑reactivity or background interference observed.
• Utilizes 100× detection antibody and 100× streptavidin‑HRP to ensure signal amplification.
• Complete reagent set includes 48‑well or 96‑well microplates to accommodate varying throughput needs.

Kit Components

• Mouse SCF Microplate
• Mouse SCF Standard (lyophilized)
• Assay Buffer (10×)
• Mouse SCF Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Assay Principle

The kit is based on a double‑antibody sandwich format:

1. Pre‑coated anti‑mouse SCF capture antibody binds SCF present in samples or standards.
2. Wash to remove unbound components.
3. Add HRP‑conjugated detection antibody to form a secondary antibody complex.
4. After a second wash, add TMB substrate; HRP catalyzes a blue color development.
5. Add stop solution, converting the color to yellow; read absorbance at 450 nm.

Applicable Sample Types

• Cell culture supernatant
• Serum
• Plasma

Performance Specifications

Detection Range	7.81 pg/mL – 500 pg/mL
Sensitivity	7.81 pg/mL
Reactive Species	Mouse
Assay Method	Sandwich ELISA

Precautions for Use

• Do not mix reagents from different lots or expired reagents.
• Allow reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips during assay to avoid cross‑contamination.
• Return unused plate strips to their packaging and store sealed at 4 °C.
• Mixing has a significant impact on results; recommend gentle vortexing or manual inversion every 10 minutes.
• Run samples and standards in duplicate or triplicate (replicates) to improve reliability.

Storage and Shipping

• Store unopened kits protected from light at 2‑8 °C; shelf life is 24 months.
• After opening, store each component according to the instructions.
• Shipping method: cold‑chain transport with ice packs (blue ice) to preserve reagent activity.

Background Information

Stem Cell Factor (SCF) is a cytokine that binds the c‑Kit receptor and exists in both transmembrane and soluble forms. SCF plays a critical role in mast cell adhesion, migration, proliferation, and survival, and participates in the release of histamine and tryptase, influencing allergic responses. Moreover, SCF synergizes with a variety of growth factors such as IL‑3, IL‑6, IL‑11, GM‑CSF, G‑CSF, and EPO to induce myeloid and erythroid colony formation, and is involved in hematopoiesis, spermatogenesis, melanogenesis, and myocardial repair.

Specifications and Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.00	Please inquire
96 T	2,558.00	In stock (ship within 5 days)

Important Statement

This product is for research use only and is not intended for human or clinical diagnostic applications. The company assumes no liability for any patent infringement or other violations that may arise from the use of this product.

Note: The above information is subject to change due to product optimization or other factors; for the most up‑to‑date details, please contact Yakein Co., Ltd.