小鼠白介素12/白介素23 p40 ELISA定量试剂盒(I

产品概述

货号：KTE3025-1 | 产品名称：小鼠白介素12/白介素23 p40 ELISA定量试剂盒

本试剂盒是一款基于双抗体夹心法原理的高性能ELISA检测试剂盒，专门用于精准定量小鼠来源的血清、血浆或细胞培养上清样本中的IL-12/IL-23 p40蛋白。作为EliKine™核心产品线的一员，它专为细胞分析领域的基础研究与药物研发设计，提供可靠、可重复的检测数据。

核心特性与优势

• 高灵敏度与特异性：最低检测浓度可达4.69 pg/mL，检测范围为4.69 - 300 pg/mL。经过严格验证，对目标蛋白的类似物未观察到明显的交叉反应或背景干扰，确保数据的准确性。
• 优化的试剂组分：试剂盒提供包括预包被微孔板、冻干标准品、检测抗体、HRP底物在内的完整组分，开盒即用，简化实验流程。
• 可靠的检测性能：基于成熟的夹心法ELISA技术，线性范围宽，重复性好，适用于对不同浓度样本的精确定量分析。

产品背景信息

白细胞介素12 (IL-12) 和白细胞介素23 (IL-23) 是免疫调节中的关键细胞因子。IL-12是由p40和p35亚基组成的异源二聚体，而IL-23则由p40亚基与p19亚基构成。因此，p40亚基是IL-12和IL-23共有的功能性亚基，在Th1细胞分化和免疫应答中扮演核心角色。本试剂盒通过特异性捕获p40亚基，实现对IL-12和IL-23活性的综合评估，是研究自身免疫、感染性疾病及肿瘤免疫机制的理想工具。

技术参数

项目	详细内容
产品类型	ELISA试剂盒
反应性	小鼠 (Mouse)
样本类型	细胞培养上清、血清、血浆
检测方法	夹心法ELISA
灵敏度	4.69 pg/mL
检测范围	4.69 pg/mL - 300 pg/mL

试剂盒组分清单

• Mouse IL-12/IL-23p40 预包被微孔板 (Mouse IL-12/IL-23p40 Microplate)
• Mouse IL-12/IL-23p40 冻干标准品 (Mouse IL-12/IL-23p40 Standard, lyophilized)
• 标准品稀释液 (Standard Diluent)
• 10× Assay Buffer
• 100× Mouse IL-12/IL-23p40 检测抗体 (Mouse IL-12/IL-23p40 Detect Antibody)
• 100× Streptavidin-HRP
• HRP底物 (TMB)
• 终止液 (Stop Solution)
• 20× 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

订购信息

规格	价格 (人民币)	货期
48 T	1,598.0	期货（预计5个工作日内发货）
96 T	2,558.0	期货（预计5个工作日内发货）

保存与运输

• 保存建议：未启封的试剂盒请避光保存于2-8℃，保质期24个月。启用后，请参照说明书妥善保存各组分。
• 运输条件：全程蓝冰冰袋运输，确保试剂活性。

重要注意事项

• 请勿将不同批次的试剂混合使用，或使用已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 建议对样品和标准品进行复孔（双孔或三孔）检测，以提高结果的可靠性。
• 实验过程中充分的混匀至关重要，推荐使用低速振荡器或定期手动摇匀。

警告： 本品仅供研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定，生产商对此类行为不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Item No.: KTE3025-1 | Product Name: Mouse Interleukin-12/Interleukin-23 p40 ELISA Quantitation Kit

This kit is a high-performance ELISA detection kit based on the sandwich immunoassay principle, specifically designed for precise quantification of IL-12/IL-23 p40 protein in mouse serum, plasma, or cell culture supernatant samples. As a member of the EliKine™ core product line, it is tailored for basic research and drug development in cellular analysis, providing reliable and reproducible assay data.

Key Features and Advantages

• High Sensitivity and Specificity: The lowest limit of detection reaches 4.69 pg/mL, with a quantification range of 4.69 – 300 pg/mL. Rigorous validation has shown no significant cross‑reactivity or background interference with protein analogues, ensuring data accuracy.
• Optimized Reagent Components: The kit provides a complete set of components, including a pre‑coated microplate, lyophilized standards, detection antibody, and HRP substrate, ready‑to‑use upon opening, streamlining the workflow.
• Robust Assay Performance: Based on mature sandwich ELISA technology, it offers a broad linear range, excellent repeatability, and is suitable for precise quantification of samples across varying concentrations.

Product Background

Interleukin‑12 (IL‑12) and Interleukin‑23 (IL‑23) are key cytokines in immune regulation. IL‑12 is a heterodimer composed of p40 and p35 subunits, whereas IL‑23 consists of the p40 subunit paired with p19. Consequently, the p40 subunit is a shared functional component of both IL‑12 and IL‑23, playing a central role in Th1 cell differentiation and immune responses. This kit captures the p40 subunit specifically, enabling a combined assessment of IL‑12 and IL‑23 activity, and serves as an ideal tool for studying autoimmunity, infectious diseases, and tumor immunology.

Technical Specifications

Parameter	Details
Product Type	ELISA Kit
Reactivity	Mouse
Sample Types	Cell culture supernatant, serum, plasma
Assay Method	Sandwich ELISA
Sensitivity	4.69 pg/mL
Detection Range	4.69 pg/mL - 300 pg/mL

Kit Component List

• Mouse IL-12/IL-23 p40 Pre‑coated Microplate (Mouse IL-12/IL-23 p40 Microplate)
• Mouse IL-12/IL-23 p40 Lyophilized Standard (Mouse IL-12/IL-23p40 Standard, lyophilized)
• Standard Diluent (Standard Diluent)
• 10× Assay Buffer
• 100× Mouse IL-12/IL-23 p40 Detection Antibody (Mouse IL-12/IL-23p40 Detect Antibody)
• 100× Streptavidin-HRP
• HRP Substrate (TMB)
• Stop Solution (Stop Solution)
• 20× Wash Buffer (Wash Buffer)
• Plate Covers (Plate Covers)

Ordering Information

Specification	Price (CNY)	Lead Time
48 T	1,598.0	Future stock (estimated shipment within 5 business days)
96 T	2,558.0	Future stock (estimated shipment within 5 business days)

Storage and Shipping

• Storage Recommendation: Unopened kits should be protected from light and stored at 2–8 °C, with a shelf life of 24 months. After opening, follow the instruction manual for proper storage of each component.
• Shipping Conditions: Transported in a continuous blue ice pack to preserve reagent activity.

Critical Points

• Do not mix reagents from different batches or use reagents that have expired.
• Prior to use, allow all reagents to equilibrate to room temperature for approximately 30 minutes.
• To avoid cross‑contamination, always use disposable pipette tips during the assay.
• It is recommended to run samples and standards in duplicate or triplicate wells to enhance result reliability.
• Thorough mixing throughout the assay is critical; a low‑speed orbital shaker or periodic manual inversion is recommended.

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements; the manufacturer assumes no responsibility for such violations.

Note: The above information is subject to change due to product optimization or upgrades. For the most current details, please contact Yakein Co.