Granzyme B ELISA KIT 颗粒酶BELISA

产品概述

货号：KTE3018-2
产品名称：EliKine™ 小鼠颗粒酶B (Granzyme B) ELISA试剂盒

本试剂盒是一款基于双抗体夹心法原理的高灵敏度、高特异性定量检测试剂盒，专门用于精准检测小鼠来源的细胞培养上清、血清或血浆样本中的颗粒酶B (Granzyme B) 蛋白含量。作为细胞分析领域的核心产品，它为您研究免疫反应及相关疾病机制提供了可靠的工具。

核心特性与优势

• 高灵敏度检测：最低检测限可达 0.31 ng/mL，能够有效捕获样本中的低丰度目标蛋白。
• 优异的特异性：经严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰，确保检测结果的准确性。
• 宽线性范围：检测范围为 0.31 ng/mL - 20 ng/mL，满足不同浓度样本的检测需求。
• 预组装即用型试剂：试剂盒包含实验所需全部核心组分，优化配比，简化操作流程，提升实验效率。
• 稳定可靠的性能：批间差小，重复性好，为您的科研数据一致性提供有力支持。

产品背景

颗粒酶B (Granzyme B) 是一种重要的丝氨酸蛋白酶，主要存在于细胞毒性T淋巴细胞(CTLs)和自然杀伤细胞(NK细胞)的胞质颗粒中。它与穿孔素协同作用，是诱导靶细胞凋亡的关键分子。近年研究发现，其在免疫调节、炎症反应及细胞外基质重塑中也扮演着重要角色，其水平异常与多种自身免疫性疾病和病理状态密切相关。本试剂盒为深入研究小鼠模型中颗粒酶B的功能提供了精准的定量检测方案。

试剂盒组分

• 小鼠颗粒酶B预包被微孔板 (Mouse Granzyme B Microplate)
• 小鼠颗粒酶B标准品 (冻干) (Mouse Granzyme B Standard, lyophilized)
• 检测缓冲液 (10×) (Assay Buffer)
• 小鼠颗粒酶B检测抗体 (100×) (Mouse Granzyme B Detect Antibody)
• 链霉亲和素-HRP偶联物 (100×) (Streptavidin-HRP)
• HRP底物 (TMB) (HRP Substrate)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×) (Wash Buffer)
• 板盖 (Plate Covers)

规格与价格

规格	货号	价格 (人民币)	货期
48 T	KTE3018-2-48T	1,598.0	请咨询
96 T	KTE3018-2-96T	2,558.0	期货（5天内发货）

保存与运输

保存建议：未启封试剂盒请避光保存于2-8℃，保质期24个月。启用后，请参照说明书妥善保存各组分。
运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要提示

• 请严格按照操作规程进行实验，不同批号试剂请勿混用。
• 使用前请将所有试剂室温平衡约30分钟。
• 为避免交叉污染，建议使用一次性吸头。
• 推荐对样品和标准品进行复孔检测，并使用振荡器混匀，以确保结果准确性。
• 本品仅供科研使用，不适用于人类或临床诊断。

如需了解更多产品详情、技术支持或订购信息，敬请垂询。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Catalog Number: KTE3018-2
Product Name: EliKine™ Mouse Granzyme B ELISA Kit

This kit is a highly sensitive and specific quantitative detection kit based on the sandwich ELISA principle, specifically designed for the accurate detection of Granzyme B protein levels in mouse-derived cell culture supernatants, serum, or plasma samples. As a core product in the field of cell analysis, it provides a reliable tool for your research on immune responses and related disease mechanisms.

Core Features and Advantages

• High Sensitivity Detection: The minimum detection limit can reach 0.31 ng/mL, effectively capturing low-abundance target proteins in samples.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with analogs of the target protein, ensuring the accuracy of detection results.
• Wide Linear Range: The detection range is 0.31 ng/mL - 20 ng/mL, meeting the detection needs of samples with different concentrations.
• Pre-assembled Ready-to-Use Reagents: The kit contains all essential core components required for the experiment, with optimized ratios to simplify the operation process and improve experimental efficiency.
• Stable and Reliable Performance: Low inter-assay variation and good reproducibility provide strong support for the consistency of your scientific research data.

Product Background

Granzyme B is an important serine protease primarily found in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It acts synergistically with perforin and is a key molecule in inducing target cell apoptosis. Recent studies have shown that it also plays important roles in immune regulation, inflammatory responses, and extracellular matrix remodeling. Abnormal levels of Granzyme B are closely associated with various autoimmune diseases and pathological conditions. This kit provides a precise quantitative detection solution for in-depth research on the function of Granzyme B in mouse models.

Kit Components

• Mouse Granzyme B Pre-coated Microplate
• Mouse Granzyme B Standard (lyophilized)
• Assay Buffer (10×)
• Mouse Granzyme B Detect Antibody (100×)
• Streptavidin-HRP Conjugate (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Specifications and Pricing

Specification	Catalog Number	Price (RMB)	Availability
48 T	KTE3018-2-48T	1,598.0	Please Inquire
96 T	KTE3018-2-96T	2,558.0	Future Goods (Ships within 5 days)

Storage and Shipping

Storage Recommendation: Store the unopened kit protected from light at 2-8°C. The shelf life is 24 months. After opening, please store each component properly according to the instructions.
Shipping Conditions: The entire shipment uses ice packs (blue ice) for low-temperature transport to ensure reagent activity.

Important Notes

• Please strictly follow the operating procedures for experiments. Do not mix reagents from different lot numbers.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• To avoid cross-contamination, the use of disposable pipette tips is recommended.
• It is recommended to perform duplicate wells for samples and standards and use a shaker for mixing to ensure result accuracy.
• This product is for research use only. Not intended for human or clinical diagnostic use.

For more product details, technical support, or ordering information, please feel free to contact us.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult ACAFBIO for specific information.