大鼠糖化血红蛋白A1c ELISA定量试剂盒(GHbA1C/

产品概述

英文名称：Rat Glycated Hemoglobin A1C (GHbA1C) ELISA Kit (One Step Method)
中文名称：大鼠糖化血红蛋白A1c ELISA定量试剂盒 (GHbA1C/HbA1c)
货号：KTE4058-1
适用物种：大鼠（血清、血浆、组织、细胞上清及相关液体样本）

核心应用

本试剂盒专为ELISA平台设计，适用于糖尿病、代谢综合征及血红蛋白相关研究中的大鼠糖化血红蛋白A1c（GHbA1c）定量检测。

检测原理

采用双抗体夹心法，步骤概览：

1. 微孔板预包被纯化的大鼠GHbA1c抗体（固相捕获抗体）。
2. 加入样本或标准品，使GHbA1c与捕获抗体结合。
3. 加入生物标记的检测抗体形成抗原‑抗体‑抗体复合物。
4. 链霉亲和素‑HRP结合检测抗体，随后加入TMB底物显色。
5. 在酸性停止液作用下颜色由蓝转黄，使用酶标仪在450 nm读取OD值，依据标准曲线计算浓度。

关键组件

性能参数

• 检测范围：0.25 μmol/L – 20 μmol/L
• 灵敏度（LOD）：0.05 μmol/L
• 特异性：未检测到显著交叉反应或背景干扰
• 重复性：建议每个样本设置双孔或三孔（复孔）以降低实验误差

使用指南（重点提示）

• 不同批号或过期的试剂不可混用。
• 使用前请将所有试剂在室温（≈20 ℃）平衡30 分钟。
• 实验全过程推荐使用一次性移液器头，防止交叉污染。
• 洗板后未用完的板条请立即放回原包装，密封后在4 ℃保存。
• 混匀是关键步骤，建议使用低速振荡器或每10 分钟手动轻摇一次。
• 样品和标准品建议采用双孔或三孔检测，以保证数据可靠性。

保存与运输

未开封试剂盒：避光、2 – 8 ℃保存，保质期6个月。
启用后各组分：请参照说明书中具体的保存条件。
运输方式：使用蓝色冰袋冷链运输，确保运输全程维持低温。

规格与报价

法规声明

本试剂盒仅限于科研用途，不得用于人类临床诊断或治疗。用户须自行确保使用过程不侵犯任何专利或许可证，因违规使用导致的后果本公司不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Rat Glycated Hemoglobin A1C (GHbA1C) ELISA Kit (One Step Method)
Chinese Name: Rat Glycated Hemoglobin A1c ELISA Quantitative Kit (GHbA1C/HbA1c)
Catalog Number: KTE4058-1
Suitable Species: Rat (serum, plasma, tissue, cell supernatant, and related liquid samples)

Core Applications

This kit is specifically designed for the ELISA platform and is suitable for the quantitative detection of rat glycated hemoglobin A1c (GHbA1c) in diabetes, metabolic syndrome, and hemoglobin-related research.

Detection Principle

Utilizes a sandwich enzyme-linked immunosorbent assay method. Procedure overview:

1. Microplate is pre-coated with purified rat GHbA1c antibody (solid-phase capture antibody).
2. Add samples or standards, allowing GHbA1c to bind to the capture antibody.
3. Add biotin-labeled detection antibody to form an antigen-antibody-antibody complex.
4. Streptavidin-HRP binds to the detection antibody, followed by the addition of TMB substrate for color development.
5. The color changes from blue to yellow upon addition of the acidic stop solution. Measure the OD at 450 nm using a microplate reader and calculate the concentration based on the standard curve.

Key Components

Performance Parameters

• Detection Range: 0.25 μmol/L – 20 μmol/L
• Sensitivity (LOD): 0.05 μmol/L
• Specificity: No significant cross-reactivity or background interference detected.
• Repeatability: It is recommended to set up duplicate or triplicate wells for each sample to minimize experimental error.

User Guide (Key Points)

• Do not mix reagents from different lots or expired reagents.
• Equilibrate all reagents to room temperature (≈20 ℃) for 30 minutes before use.
• Use disposable pipette tips throughout the experiment to prevent cross-contamination.
• After washing, immediately return unused strips to the original packaging, seal, and store at 4 ℃.
• Thorough mixing is a critical step; use a low-speed shaker or manually shake gently every 10 minutes.
• It is recommended to test samples and standards in duplicate or triplicate to ensure data reliability.

Storage and Shipping

Unopened Kit: Store protected from light at 2 – 8 ℃; shelf life is 6 months.
Opened Components: Please refer to the specific storage conditions in the instruction manual.
Shipping Method: Shipped with blue ice packs via cold chain to ensure low temperature throughout transit.

Specifications and Pricing

Regulatory Statement

This kit is intended for research use only and is not for use in human clinical diagnosis or treatment. Users must ensure that their use does not infringe upon any patents or licenses. The company is not responsible for consequences resulting from non-compliant use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Ya Keyin Company.