大鼠免疫球蛋白M ELISA定量试剂盒(IgM)

EliKine™ 大鼠免疫球蛋白M (IgM) ELISA定量试剂盒

货号：KTE3132-1

产品概述

EliKine™ 大鼠免疫球蛋白M (IgM) ELISA定量试剂盒是一款基于经典双抗体夹心法原理开发的检测试剂盒，专门设计用于定量检测大鼠血清、血浆或细胞培养上清样本中的IgM含量。本试剂盒隶属于蛋白类核心产品线，在ELISA应用领域表现出卓越的性能，是进行细胞免疫分析研究的可靠工具。

产品特点与优势

• 高灵敏度与宽检测范围：检测下限低至0.63 ng/mL，定量检测范围覆盖0.63 ng/mL - 40 ng/mL，能够精准检测样本中微量的IgM浓度变化。
• 优异特异性：经过严格验证，对目标蛋白的类似物检测未观察到明显的交叉反应或背景干扰，确保检测结果的准确性与可靠性。
• 操作便捷：试剂盒提供预包被微孔板及优化的缓冲液体系，实验流程标准化，有效缩短操作时间并提升实验效率。
• 批次稳定性：严格的生产质量控制体系，保证不同批次间试剂性能的高度一致性。

检测原理

本试剂盒采用双抗体夹心ELISA法。微孔板预包被有大鼠IgM特异性抗体。加入样本或标准品后，其中的大鼠IgM会与固相抗体结合。洗板后加入HRP标记的检测抗体形成“夹心”复合物。再次洗涤后加入TMB底物进行显色反应，颜色的深浅与样本中的大鼠IgM浓度成正比。

试剂盒组分

• 预包被大鼠IgM微孔板 (Rat IgM Microplate)
• 大鼠IgM标准品 (冻干粉) (Rat IgM Standard, lyophilized)
• 检测缓冲液 (10×) (Assay Buffer)
• HRP标记的大鼠IgM检测抗体 (100×) (HRP-conjugated Rat IgM Detect Antibody)
• HRP底物 (TMB) (HRP Substrate)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (20×) (Wash Buffer)
• 板盖 (Plate Covers)

规格与价格

规格	价格 (元)	货期
48 T	1598.0	请咨询
96 T	2558.0	期货（5天内发货）

样本类型

适用于大鼠的细胞培养上清、血清、血浆样本。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条应立即放回原包装袋中密封，并于4℃保存。
• 为确保反应充分，建议在振荡器上低速混匀或每10分钟手动摇匀。
• 为获得更准确的结果，推荐对样品和标准品进行双孔或三孔复孔检测。

保存与运输

保存：未启封的试剂盒请避光保存于2-8℃，有效期为24个月。启用后，各组分的具体保存条件请参阅说明书。

运输：本产品采用冰袋（蓝冰）运输，以确保试剂活性。

背景信息

免疫球蛋白M (IgM)是B细胞在受到抗原刺激后产生的抗体类型，也是人体循环系统中分子量最大的抗体。它主要存在于血清中，并在感染初期发挥重要的免疫防御作用。检测血清中IgM的水平对于判断近期感染或某些疾病的早期诊断具有重要的参考价值。本试剂盒为研究大鼠免疫应答，特别是早期体液免疫反应提供了有效的检测手段。

警告： 本产品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Rat Immunoglobulin M (IgM) ELISA Quantitation Kit

Catalog Number: KTE3132-1

Product Overview

The EliKine™ Rat Immunoglobulin M (IgM) ELISA Quantitation Kit is a detection kit developed based on the classic double-antibody sandwich principle, specifically designed for the quantitative measurement of IgM levels in rat serum, plasma, or cell culture supernatant samples. This kit belongs to the Protein core product line and demonstrates excellent performance in the field of ELISA applications, making it a reliable tool for cellular immune analysis research.

Features & Advantages

• High Sensitivity & Broad Detection Range: The minimum detectable concentration is as low as 0.63 ng/mL, with a quantitative detection range covering 0.63 ng/mL - 40 ng/mL, enabling precise detection of subtle changes in IgM concentration within samples.
• Excellent Specificity: Rigorously validated, no significant cross-reactivity or background interference with analogs of the target protein has been observed, ensuring the accuracy and reliability of the results.
• Convenient Operation: The kit provides pre-coated microplates and an optimized buffer system, featuring a standardized experimental procedure that effectively reduces hands-on time and improves experimental efficiency.
• Lot-to-Lot Consistency: Strict production quality control systems ensure high consistency in reagent performance across different lots.

Assay Principle

This kit employs a double-antibody sandwich ELISA method. The microplate is pre-coated with a rat IgM-specific antibody. After adding samples or standards, the rat IgM present binds to the immobilized antibody. Following a wash step, an HRP-conjugated detection antibody is added to form a "sandwich" complex. After another wash, TMB substrate is added for the color development reaction. The intensity of the color developed is proportional to the concentration of rat IgM in the sample.

Kit Components

• Pre-coated Rat IgM Microplate
• Rat IgM Standard (lyophilized powder)
• Assay Buffer (10×)
• HRP-conjugated Rat IgM Detection Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Specifications & Price

Specification	Price (CNY)	Availability
48 T	1598.0	Please inquire
96 T	2558.0	Futures (Ships within 5 days)

Sample Types

Suitable for rat cell culture supernatant, serum, and plasma samples.

Important Notes

• Do not mix reagents from different lots or expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• To avoid cross-contamination, use disposable pipette tips throughout the procedure.
• Unused strips should be immediately returned to the original foil pouch, sealed, and stored at 4°C.
• To ensure sufficient reaction, low-speed agitation on a plate shaker or manual shaking every 10 minutes is recommended.
• For more accurate results, it is recommended to test samples and standards in duplicate or triplicate wells.

Storage & Shipping

Storage: The unopened kit should be stored away from light at 2-8°C; the shelf life is 24 months. After opening, please refer to the manual for specific storage conditions for each component.

Shipping: This product is shipped with ice packs (blue ice) to ensure reagent activity.

Background Information

Immunoglobulin M (IgM) is the first type of antibody produced by B cells upon antigenic stimulation and is the largest antibody by molecular weight in the circulatory system. It is primarily found in serum and plays a crucial role in immune defense during the early stages of infection. Measuring serum IgM levels provides valuable reference information for determining recent infections or early diagnosis of certain diseases. This kit provides an effective tool for studying rat immune responses, particularly early humoral immune responses.

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. The user should ensure that its application does not infringe any patents or license agreements. The company assumes no liability for any patent infringement or other violations that may result from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult YaKeyin Company.