大鼠脂联素 ELISA定量试剂盒(Acrp30)

大鼠脂联素 (Acrp30) ELISA定量试剂盒 - KTE3127-1

英文名称：Rat Adiponectin/Acrp30 ELISA Kit

产品分类：核心产品 | 细胞分析 | ELISA（夹心法）

适用物种：大鼠

检测类型：ELISA（双抗体夹心法）

产品背景与应用价值

脂联素（Acrp30）是一种由脂肪组织和胎盘分泌的蛋白质激素，参与葡萄糖调节、脂肪酸氧化以及抗炎、抗动脉粥样硬化等代谢过程。监测大鼠血清、血浆或细胞培养上清中的脂联素水平，可帮助研究者：

• 评估2型糖尿病、肥胖、非酒精性脂肪肝等代谢性疾病模型的代谢状态。
• 研究脂联素在炎症调节、巨噬细胞TNF‑α产生抑制及血管内皮功能中的作用。
• 筛选能够调节Acrp30表达或分泌的药物候选分子。

关键技术特性

• 极高灵敏度：检测下限 78.13 pg/mL。
• 宽测定范围：78.13 pg/mL – 5,000 pg/mL，满足低至中等浓度样本的定量需求。
• 高特异性：未检测到明显交叉反应或背景干扰。
• 双抗体夹心法：采用特异性包被抗体、双抗体-生物素/链霉亲和素-HRP体系，信号放大且可重复性好。

试剂盒组成（每盒）

• Rat Adiponectin Microplate
• Rat Adiponectin Standard (lyophilized)
• Assay Buffer (10×)
• Rat Adiponectin Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

典型实验流程（概览）

1. 将标准品或样品（血清、血浆、细胞培养上清）加入已包被抗体的酶标板孔中，37 °C孵育1 h。
2. 洗涤去除未结合组分。
3. 加入生物素化检测抗体，37 °C孵育30 min。
4. 洗涤后加入链霉亲和素‑HRP，室温孵育30 min。
5. 洗涤并加入TMB显色底物，显色后加入终止液。
6. 在450 nm读取OD值，使用标准曲线计算样本中Acrp30浓度。

为确保数据可靠，建议每个样本设定双孔或三孔复测。

性能指标

检测范围	78.13 pg/mL – 5,000 pg/mL
灵敏度 (LOD)	78.13 pg/mL
样本类型	细胞培养上清、血清、血浆

使用注意事项

• 同一批号内的试剂方可混用，禁用不同批号或过期试剂。
• 使用前将所有试剂在室温平衡30 min。
• 实验中请使用一次性吸头，防止交叉污染。
• 未使用的板条需立即密封回包装，4 °C保存。
• 每10 min轻轻摇匀或使用低速振荡器，以确保充分混合。
• 标准品与样品建议做双孔/三孔复测，提高可靠性。

保存与运输

• 未开封的整盒请避光保存于2‑8 °C，保质期24 个月。
• 启封后各组分请严格按照说明书保存条件进行管理。
• 运输方式：冰袋（蓝冰）配合冷链运输。

法规声明

本试剂盒仅供科研使用，不适用于人类或临床诊断。使用本产品进行的任何专利或许可证相关行为，风险需自行承担。

订购信息

规格	包装数量	单价 (CNY)	交货期
48 T	48	1,598.0	期货（5天内发货）
96 T	96	2,558.0	期货（5天内发货）

产品货号：KTE3127-1  |  基因/蛋白别名：Acrp30 / 脂联素  |  检测指标：Acrp30 / 脂联素

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat Adiponectin (Acrp30) ELISA Quantitation Kit - KTE3127-1

English Name:Rat Adiponectin/Acrp30 ELISA Kit

Product Category:Core Products | Cell Analysis | ELISA (Sandwich)

Applicable Species:Rat

Assay Type:ELISA (Dual-Antibody Sandwich)

Product Background and Application Value

Adiponectin (Acrp30) is a protein hormone secreted by adipose tissue and placenta, involved in glucose regulation, fatty‑acid oxidation, and anti‑inflammatory, anti‑atherosclerotic metabolic processes. Monitoring adiponectin levels in rat serum, plasma, or cell‑culture supernatants can assist investigators to:

• Assess the metabolic status of type‑2 diabetes, obesity, non‑alcoholic fatty liver disease, and other metabolic disease models.
• Investigate the role of adiponectin in inflammation regulation, suppression of macrophage TNF‑α production, and vascular endothelial function.
• Screen drug candidates that modulate Acrp30 expression or secretion.

Key Technical Features

• Ultra‑high Sensitivity: Limit of detection 78.13 pg/mL.
• Wide Dynamic Range: 78.13 pg/mL – 5,000 pg/mL, suitable for quantifying low to moderate concentration samples.
• High Specificity: No significant cross‑reactivity or background interference detected.
• Dual‑Antibody Sandwich Format: Utilizes a specific capture antibody and a biotin‑labeled detection antibody with streptavidin‑HRP, providing signal amplification and excellent reproducibility.

Kit Components (per kit)

• Rat Adiponectin Microplate
• Rat Adiponectin Standard (lyophilized)
• Assay Buffer (10×)
• Rat Adiponectin Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Typical Experimental Procedure (Overview)

1. Add standards or samples (serum, plasma, cell‑culture supernatant) to wells pre‑coated with capture antibody, incubate at 37 °C for 1 h.
2. Wash to remove unbound components.
3. Add biotinylated detection antibody, incubate at 37 °C for 30 min.
4. Wash, then add streptavidin‑HRP, incubate at room temperature for 30 min.
5. Wash and add TMB substrate; after color development, add stop solution.
6. Read absorbance at 450 nm and calculate Acrp30 concentration using the standard curve.

To ensure data reliability, it is recommended to run each sample in duplicate or triplicate.

Performance Specifications

Detection Range	78.13 pg/mL – 5,000 pg/mL
Sensitivity (LOD)	78.13 pg/mL
Sample Type	Cell‑culture supernatant, serum, plasma

Precautions

• Only reagents from the same lot may be interchanged; do not use reagents from different lots or expired reagents.
• Allow all reagents to equilibrate to room temperature for 30 min before use.
• Use disposable pipette tips during the assay to avoid cross‑contamination.
• Unutilized plate strips must be resealed promptly and stored at 4 °C.
• Gently mix every 10 min or use a low‑speed shaker to ensure thorough mixing.
• Perform standards and samples in duplicate or triplicate to improve reliability.

Storage and Shipping

• Store unopened boxes protected from light at 2‑8 °C; shelf life is 24 months.
• After opening, each component must be stored according to the conditions specified in the manual.
• Shipping: ice packs (blue ice) with cold‑chain logistics.

Regulatory Statement

This kit is for research use only and is not intended for human or clinical diagnostic applications. Any patent or licensing activities associated with the use of this product are undertaken at the user's own risk.

Ordering Information

Specification	Package Qty	Unit Price (CNY)	Lead Time
48 T	48	1,598.0	Stock (shipment within 5 days)
96 T	96	2,558.0	Stock (shipment within 5 days)

Product Code:KTE3127-1  |  Gene/Protein Alias:Acrp30 / Adiponectin  |  Assay Target:Acrp30 / Adiponectin

Note: The above information is subject to change due to product upgrades or other factors; please contact Yakeein Co. for the most current details.