大鼠脂联素 ELISA定量试剂盒(ADP/GBP28/ApM

大鼠脂联素(ADP) ELISA定量试剂盒 - KTE4049-1

本试剂盒专为大鼠样本中脂联素（Adiponectin，ADP）的精确定量而设计，采用一步法操作流程，是代谢研究、肥胖及相关疾病研究领域的理想工具。

产品概述

EliKine™ 大鼠脂联素(ADP) ELISA定量试剂盒采用双抗体夹心法原理，用于定量检测大鼠血清、血浆、组织匀浆及细胞上清液等样本中的脂联素蛋白水平。该试剂盒具有卓越的检测性能，为科研人员提供可靠的数据支持。

核心特性与优势

• 高灵敏度：最低检测浓度可达0.02 ng/mL，能够精准检测样本中的低丰度脂联素。
• 宽线性范围：检测范围为0.1 ng/mL - 8 ng/mL，覆盖广泛的生理和病理浓度。
• 优异特异性：经过严格验证，对目标蛋白类似物无明显交叉反应或背景干扰，确保结果准确性。
• 操作便捷：优化的“一步法”流程，减少了操作步骤和时间，提高了实验效率。

检测原理

试剂盒采用双抗体夹心ELISA法。预包被于酶标板的特异性抗体捕获样本中的大鼠脂联素，随后依次加入生物素化检测抗体和链霉亲和素-HRP，形成复合物。加入TMB底物显色，显色强度与样本中的脂联素浓度成正比。

试剂盒组分

组分名称	规格
预包被酶标板 (Rat Adiponectin Microplate)	96孔 / 48孔
脂联素标准品 (Rat Adiponectin Standard, 16 ng/mL)	2支
标准品稀释液 (Standard Diluent)	1瓶
检测抗体 (Rat Adiponectin Detect Antibody)	1瓶
链霉亲和素-HRP (Streptavidin-HRP)	1瓶
底物A (HRP Substrate A) & 底物B (HRP Substrate B)	各1瓶
终止液 (Stop Solution)	1瓶
洗涤缓冲液 (Wash Buffer, 浓缩液)	1瓶
封板膜 (Plate Covers)	4张

样本类型

适用于大鼠的血清、血浆、组织分浆、细胞培养上清液及其他相关液体样本。

规格与价格

规格	价格 (人民币)	货期
48 T	1,998.0	期货（预计3个工作日内发货）
96 T	3,198.0	期货（预计3个工作日内发货）

保存与运输

• 保存：未开封试剂盒请避光保存于2-8℃，保质期6个月。启用后各组分请按说明书建议保存。
• 运输：全程采用冰袋（蓝冰）低温运输，确保试剂活性。

重要注意事项

• 请勿将不同批次的试剂混合使用，亦请勿使用过期试剂。
• 使用前，所有试剂需室温平衡约30分钟。
• 实验过程中请使用一次性耗材，避免交叉污染。
• 建议样本和标准品进行复孔检测（双孔或三孔），以提高数据可靠性。
• 充分混匀是确保结果准确的关键步骤。

警告：本产品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或法规。

别名信息： Adiponectin, ADP, GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat Adiponectin (ADP) ELISA Quantification Kit - KTE4049-1

This kit is specifically designed for the precise quantification of adiponectin (ADP) in rat samples, featuring a one-step operational procedure. It is an ideal tool for research in metabolism, obesity, and related diseases.

Product Overview

EliKine™ Rat Adiponectin (ADP) ELISA Quantification Kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) principle for the quantitative detection of adiponectin protein levels in rat serum, plasma, tissue homogenates, cell culture supernatants, and other sample types. The kit offers excellent detection performance, providing reliable data support for researchers.

Key Features and Advantages

• High Sensitivity: Minimum detectable concentration as low as 0.02 ng/mL, enabling accurate detection of low-abundance adiponectin in samples.
• Wide Linear Range: Detection range from 0.1 ng/mL to 8 ng/mL, covering a broad spectrum of physiological and pathological concentrations.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with analogous proteins, ensuring result accuracy.
• Operational Convenience: Optimized "one-step" procedure reduces operational steps and time, enhancing experimental efficiency.

Detection Principle

The kit employs a sandwich ELISA method. A specific antibody pre-coated on the microplate captures rat adiponectin from the sample. Subsequently, a biotinylated detection antibody and Streptavidin-HRP are added sequentially to form a complex. Following the addition of TMB substrate, color development occurs, with the intensity being proportional to the adiponectin concentration in the sample.

Kit Components

Component Name	Specification
Pre-coated Microplate (Rat Adiponectin Microplate)	96 wells / 48 wells
Adiponectin Standard (Rat Adiponectin Standard, 16 ng/mL)	2 vials
Standard Diluent	1 bottle
Detection Antibody (Rat Adiponectin Detect Antibody)	1 vial
Streptavidin-HRP	1 vial
Substrate A (HRP Substrate A) & Substrate B (HRP Substrate B)	1 bottle each
Stop Solution	1 bottle
Wash Buffer (Concentrate)	1 bottle
Plate Covers	4 sheets

Sample Types

Suitable for rat serum, plasma, tissue homogenates, cell culture supernatants, and other related liquid samples.

Specifications and Pricing

Specification	Price (RMB)	Lead Time
48 T	1,998.0	Future goods (Expected shipment within 3 business days)
96 T	3,198.0	Future goods (Expected shipment within 3 business days)

Storage and Shipping

• Storage: Store the unopened kit protected from light at 2-8°C. Shelf life is 6 months. After opening, store components as recommended in the instructions.
• Shipping: Shipped with ice packs (blue ice) to maintain low temperature throughout transit, ensuring reagent activity.

Important Notes

• Do not mix reagents from different lots, and do not use expired reagents.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable consumables during the experiment to avoid cross-contamination.
• It is recommended to perform duplicate or triplicate assays for samples and standards to improve data reliability.
• Thorough mixing is a critical step to ensure accurate results.

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Users should ensure their application does not infringe upon relevant patents or regulations.

Alias Information: Adiponectin, ADP, GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.