血管内皮生长因子酶联免疫试剂盒(大鼠) VEGF ELISA

产品概述

EliKine™ 大鼠血管内皮生长因子(VEGF)酶联免疫吸附测定(ELISA)试剂盒（货号：KTE9008-3）是一款专为大鼠样本设计的核心产品，采用高灵敏度的夹心法ELISA原理，用于精准定量检测细胞培养上清、血清、血浆及其他生物液体中的VEGF蛋白水平。本试剂盒凭借其卓越的性能，为心血管生物学、肿瘤学及代谢疾病等领域的研究提供可靠的数据支持。

主要特性与优势

• 高灵敏度：最低检测浓度达8 pg/mL，可精准检测低丰度样本。
• 优异特异性：经严格验证，与目标蛋白类似物无明显交叉反应或背景干扰。
• 宽线性范围：检测范围为15.6-1000 pg/mL，覆盖多种样本浓度需求。
• 即用型组分：预包被微孔板及优化试剂，简化操作流程，提升实验效率。
• 严格的质控标准：每批次试剂均经过性能验证，确保结果的一致性。

试剂盒组分（48T/96T）

• 大鼠VEGF抗体预包被微孔板
• 大鼠VEGF蛋白标准品
• 大鼠VEGF检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液、实验缓冲液、HRP底物、终止液
• 洗涤缓冲液、封板膜

背景信息

血管内皮生长因子（VEGF）是一种分子量约34-45 kDa的同源二聚体糖蛋白，又称血管通透性因子（VPF），在促进血管生成、内皮细胞增殖及血管通透性调节中发挥关键作用。其与大鼠模型中的肿瘤转移、生殖调控及糖尿病并发症等研究密切相关，基因ID为83785（UniProt ID: P16612）。

技术参数

项目	参数
检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆、其他生物液体
检测范围	15.6-1000 pg/mL
灵敏度	8 pg/mL
反应种属	大鼠
保存条件	未开封试剂盒2-8℃储存；启用后参照说明书保存各组分
运输条件	冰袋（蓝冰）运输

规格与价格

• 48T：价格1598.0元 | 货期：现货（次日发货）
• 96T：价格2558.0元 | 货期：现货（次日发货）

注意事项

• 请勿混用不同批号或过期试剂。
• 使用前需室温平衡30分钟，推荐使用一次性吸头避免污染。
• 未使用的板条需密封后4℃保存。
• 建议使用振荡器混匀试剂，并对样本及标准品进行复孔检测以提高准确性。

警告：本产品仅用于科研用途，不适用于临床诊断。使用前请确保符合相关法规及知识产权要求。

别名参考：VEGF-A, VPF, vascular permeability factor, 大鼠VEGF ELISA试剂盒, KET9008

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

EliKine™ Rat Vascular Endothelial Growth Factor (VEGF) Enzyme-Linked Immunosorbent Assay (ELISA) Kit (Catalog No.: KTE9008-3) is a core product specifically designed for rat samples. It employs a highly sensitive sandwich‑ELISA format for precise quantitative detection of VEGF protein levels in cell culture supernatants, serum, plasma, and other biological fluids. Owing to its outstanding performance, this kit provides reliable data support for research in cardiovascular biology, oncology, metabolic diseases, and related fields.

Key Features and Advantages

• High Sensitivity: Minimum detection limit of 8 pg/mL, enabling accurate measurement of low‑abundance samples.
• Excellent Specificity: Rigorously validated with no significant cross‑reactivity or background interference from homologous proteins.
• Broad Linear Range: Quantification range of 15.6–1000 pg/mL, covering diverse sample concentration requirements.
• Ready‑to‑Use Components: Pre‑coated microplate and optimized reagents streamline workflow and increase throughput.
• Strict Quality Control: Every batch undergoes performance verification to ensure result consistency.

Kit Components (48T/96T)

• Rat VEGF antibody‑coated microplate
• Rat VEGF protein standards
• Rat VEGF detection antibody
• EliKine™ Streptavidin‑HRP
• Standard diluent, assay buffer, HRP substrate, stop solution
• Wash buffer, plate sealing film

Background Information

Vascular Endothelial Growth Factor (VEGF) is a homologous dimeric glycoprotein of approximately 34–45 kDa, also known as Vascular Permeability Factor (VPF). It plays a pivotal role in angiogenesis, endothelial cell proliferation, and regulation of vascular permeability. VEGF is closely linked to studies of tumor metastasis, reproductive regulation, and diabetic complications in rat models. Gene ID: 83785 (UniProt ID: P16612).

Technical Specifications

Item	Specification
Assay Method	Sandwich ELISA
Sample Types	Cell culture supernatant, serum, plasma, other biological fluids
Quantitation Range	15.6–1000 pg/mL
Sensitivity	8 pg/mL
Species Reactivity	Rat
Storage Conditions	Unopened kit: 2–8 °C; post‑opening: store individual components as indicated in the manual
Shipping Conditions	Transport with ice packs (blue ice)

Specifications and Pricing

• 48T: ¥1598.0 | Availability: In stock (next‑day shipment)
• 96T: ¥2558.0 | Availability: In stock (next‑day shipment)

Precautions

• Do not mix reagents from different lot numbers or use expired components.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use; use disposable tips to avoid contamination.
• Seal unused plate strips and store at 4 °C.
• Mix reagents on a vortexer and perform duplicate measurements of samples and standards to improve accuracy.

Warning: This product is for research use only and is not intended for clinical diagnosis. Ensure compliance with applicable regulations and intellectual‑property requirements before use.

Synonym Reference: VEGF‑A, VPF, vascular permeability factor, Rat VEGF ELISA Kit, KET9008

Note: The above information may change due to product optimization or upgrades. For the most current details, please contact EliKine™.