小鼠胰岛素样生长因子1 ELISA定量试剂盒(IGF-1)

Mouse/Rat IGF‑1 ELISA Kit (KTE3036‑1)

中文名称：小鼠胰岛素样生长因子‑1 ELISA定量试剂盒（IGF‑1）
商品编号：KTE3036‑1
适用物种：小鼠 / 大鼠

产品概览

本套装属于EliKine™核心产品系列，专为细胞分析与蛋白水平定量设计，采用高灵敏度的双抗体夹心ELISA技术，实现对IGF‑1的精准测定。适用于细胞培养上清、血清及血浆等多种样本基质。

关键技术参数

• 检测类型：酶联免疫吸附测定（ELISA）
• 检测方法：夹心法
• 检测范围：15.63 pg/mL – 1,000 pg/mL
• 检测下限（灵敏度）：15.63 pg/mL
• 样本类型：细胞培养上清、血清、血浆
• 测定波长：450 nm（参考波长 570‑630 nm）

试剂盒组成

• Mouse/Rat IGF‑1 Microplate（预包被高亲和力抗体）
• Mouse/Rat IGF‑1 Standard (lyophilized)
• Assay Buffer (10×)
• Mouse/Rat IGF‑1 Detect Antibody (100×, biotin‑标记)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

特点与优势

• 高灵敏度（≤15.63 pg/mL）满足低丰度IGF‑1检测需求。
• 特异性抗小鼠/大鼠IGF‑1抗体，交叉反应与背景干扰极低。
• 双抗体夹心结构提供宽动态范围，适用于浓度变化大的实验样本。
• 完整的实验步骤配套试剂，降低实验操作变量。

实验操作要点

• 禁止将不同批号或已过期的试剂混用。
• 使用前请将所有试剂在室温（≈25 °C）平衡30 分钟。
• 建议使用一次性吸头，防止交叉污染。
• 未使用的板条需立即放回原包装，密封后在4 °C保存。
• 反应混合过程需充分均匀，推荐低速振荡或每10 分钟手动轻摇一次。
• 标准品及样本建议设置双孔或三孔复孔，以提高数据可靠性。

存储与运输

• 未开封保存：避光、2‑8 °C，保质期24个月。
• 启用后：请参照说明书对各组分进行分装保存。
• 运输方式：冰袋（蓝冰）冷链运输，确保全程温度控制。

安全与合规

本产品仅供科研使用，不适用于临床诊断或人类治疗。使用过程中请遵守所在机构及当地的专利、许可证规定，因本产品引发的任何专利侵权或违规行为，本公司概不负责。

IGF‑1 背景信息

胰岛素样生长因子‑1（IGF‑1）是一种结构类似胰岛素的多功能激素，主要在肝脏合成并作为内分泌因子发挥作用。IGF‑1 能激活AKT信号通路，促进细胞增殖、抑制细胞凋亡，并通过IGF‑1受体（IGF1R）或胰岛素受体介导信号传导。其表达受生长激素、营养状态以及下游信号（如STAT5B）调控。IGF‑1 在儿童生长、成人代谢、衰老、神经退行性疾病、癌症及中风等多种生理病理过程中发挥关键作用，因而成为细胞生物学、药理学及代谢研究中的常规检测指标。

包装规格与采购信息

规格	含量（测试孔）	单价（人民币）	交货期
48 T	48 孔	1,598.00	期货（5 天内发货）
96 T	96 孔	2,558.00	期货（5 天内发货）

订购与技术支持

如需进一步的技术指导、样本前处理建议或批量采购优惠，请联系贵司专属客户经理或访问我们的技术支持平台。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse/Rat IGF‑1 ELISA Kit (KTE3036‑1)

Product Name: Mouse Insulin-like Growth Factor‑1 ELISA Quantitative Kit (IGF‑1)
Catalog Number: KTE3036‑1
Applicable Species: Mouse / Rat

Product Overview

This kit belongs to the EliKine™ core product series, specifically designed for cell analysis and protein level quantification. It utilizes a highly sensitive double-antibody sandwich ELISA technology for the accurate determination of IGF‑1. It is suitable for various sample matrices including cell culture supernatants, serum, and plasma.

Key Technical Parameters

• Assay Type: Enzyme-Linked Immunosorbent Assay (ELISA)
• Assay Format: Sandwich
• Detection Range: 15.63 pg/mL – 1,000 pg/mL
• Lower Limit of Detection (Sensitivity): 15.63 pg/mL
• Sample Types: Cell Culture Supernatant, Serum, Plasma
• Detection Wavelength: 450 nm (Reference Wavelength 570‑630 nm)

Kit Components

• Mouse/Rat IGF‑1 Microplate (Pre-coated with high-affinity antibody)
• Mouse/Rat IGF‑1 Standard (lyophilized)
• Assay Buffer (10×)
• Mouse/Rat IGF‑1 Detect Antibody (100×, biotin-labeled)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Features & Advantages

• High sensitivity (≤15.63 pg/mL) meets the detection requirements for low-abundance IGF‑1.
• Specific antibodies against mouse/rat IGF‑1, with minimal cross-reactivity and background interference.
• The double-antibody sandwich structure provides a wide dynamic range, suitable for experimental samples with large concentration variations.
• Complete set of reagents for the experimental procedure reduces operational variables.

Key Experimental Considerations

• Do not mix reagents from different lots or expired reagents.
• Allow all reagents to equilibrate to room temperature (≈25 °C) for 30 minutes before use.
• Use disposable pipette tips to prevent cross-contamination.
• Unused strips should be immediately returned to the original packaging, sealed, and stored at 4 °C.
• Reaction mixtures must be thoroughly homogenized; low-speed shaking or manual gentle shaking every 10 minutes is recommended.
• Standards and samples should be assayed in duplicate or triplicate to improve data reliability.

Storage & Shipping

• Unopened Storage: Protect from light, 2‑8 °C, shelf life 24 months.
• After Opening: Please refer to the instructions for aliquoting and storage of individual components.
• Shipping Method: Shipped with ice packs (blue ice) under cold chain conditions to ensure temperature control throughout transit.

Safety & Compliance

This product is for research use only. Not intended for clinical diagnosis or human therapeutic use. Please comply with the patent, license regulations of your institution and local authorities when using this product. The company assumes no responsibility for any patent infringement or regulatory violations arising from the use of this product.

IGF‑1 Background Information

Insulin-like Growth Factor‑1 (IGF‑1) is a multifunctional hormone structurally similar to insulin, primarily synthesized in the liver and functioning as an endocrine factor. IGF‑1 activates the AKT signaling pathway, promotes cell proliferation, inhibits apoptosis, and mediates signal transduction via the IGF‑1 receptor (IGF1R) or the insulin receptor. Its expression is regulated by growth hormone, nutritional status, and downstream signals (e.g., STAT5B). IGF‑1 plays a critical role in various physiological and pathological processes such as childhood growth, adult metabolism, aging, neurodegenerative diseases, cancer, and stroke, making it a routine detection target in cell biology, pharmacology, and metabolic research.

Packaging Specifications & Ordering Information

Specification	Content (Test Wells)	Unit Price (RMB)	Delivery Time
48 T	48 wells	1,598.00	Future goods (Ships within 5 days)
96 T	96 wells	2,558.00	Future goods (Ships within 5 days)

Ordering & Technical Support

For further technical guidance, sample pre-treatment advice, or bulk purchase discounts, please contact your dedicated account manager or visit our technical support platform.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.