Interferonβ/IFN-β/IFNB1/IFN-B/

Rat IFN-β ELISA Kit (One‑Step Method) – KTE4017‑2

产品概述

本套装为EliKine™系列核心产品，专为大鼠β干扰素（IFN‑β）定量分析设计，采用双抗体夹心法实现一次性完成的ELISA检测。适用于血清、血浆、组织匀浆、细胞上清以及其他相关液体样本的高灵敏度测定，是细胞分析与免疫学研究的常规工具。

主要特点 & 优势

• 极高灵敏度：检测下限 1 pg/mL，覆盖 5 pg/mL‑400 pg/mL 的动态范围。
• 特异性强：已验证对大鼠IFN‑β的特异性，无显著交叉反应或背景干扰。
• 一次性操作：“One‑Step”流程简化实验步骤，降低操作误差。
• 兼容性：适配常规酶标仪（450 nm），可与市售的TMB底物系统直接配合。
• 标准化配套：含有预配浓度的标准品（800 pg/mL）与稀释缓冲，确保实验重复性。

检测原理

ELISA板预包被针对大鼠IFN‑β的捕获抗体，加入样品或标准后形成抗体‑抗原复合物。随后加入生物素化的检测抗体和链霉亲和素‑HRP，完成抗体‑抗原‑抗体‑酶的四元复合物。经洗涤后加入HRP底物（TMB），在酶促下显色，终止后在450 nm读取OD值，所得光密度与IFN‑β浓度呈正比。

试剂盒组分

• Rat IFN‑β Microplate（预包被）
• Rat IFN‑β Standard (800 pg/mL)
• Standard Diluent
• Rat IFN‑β Detect Antibody (biotinylated)
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

适用样本类型

血清 / 血浆 / 组织匀浆 / 细胞培养上清及其他相关液体样本。

使用建议

• 样品与标准建议采用双孔或三孔复孔，以降低偶然误差。
• 实验前所有试剂请于室温平衡 30 分钟。
• 采用一次性吸头，防止交叉污染。
• 使用低速振荡器或每 10 分钟手动轻摇，确保混匀。
• 未使用的板条请立即回装、密封并置于 4 ℃保存。

储存与运输

未开封试剂盒：避光保存于 2‑8 ℃，有效期 6 个月。
开启后：请参考随盒说明书对各组分的分开储存要求。
运输：采用蓝冰（冰袋）冷链运输，确保全程温控。

技术规格

适用研究领域

本试剂盒适用于病毒感染、免疫调节、炎症模型以及药物筛选等涉及大鼠IFN‑β动态变化的科研项目，帮助科研人员快速获得可靠的定量数据。

重要声明

本产品仅供科研使用，不得用于人类临床诊断或治疗。使用本试剂盒的任何专利或许可证相关事宜，请自行确认并遵守相关法规；对因此产生的侵权或其他法律责任，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat IFN-β ELISA Kit (One‑Step Method) – KTE4017‑2

Product Overview

This kit is a core product of the EliKine™ series, specifically designed for the quantitative analysis of rat beta interferon (IFN‑β) using a sandwich ELISA format that completes the assay in a single step. It is suitable for high‑sensitivity measurement of serum, plasma, tissue homogenates, cell supernatants, and other related liquid samples, and serves as a routine tool for cellular analysis and immunology research.

Key Features & Advantages

• Ultra‑high sensitivity: detection limit of 1 pg/mL, covering a dynamic range of 5 pg/mL–400 pg/mL.
• Strong specificity: validated for rat IFN‑β with no significant cross‑reactivity or background interference.
• One‑Step workflow: simplifies the assay procedure and reduces operational error.
• Compatibility: works with conventional microplate readers (450 nm) and can be directly paired with commercially available TMB substrate systems.
• Standardized kit: includes a pre‑diluted standard (800 pg/mL) and dilution buffer to ensure assay reproducibility.

Assay Principle

The ELISA plate is pre‑coated with a capture antibody specific for rat IFN‑β; addition of sample or standard forms an antibody‑antigen complex. A biotinylated detection antibody and streptavidin‑HRP are then added, generating a antibody‑antigen‑antibody‑enzyme sandwich complex. After washing, HRP substrate (TMB) is added; enzymatic conversion produces color, which is stopped and read at 450 nm. The measured optical density is directly proportional to the IFN‑β concentration.

Kit Components

• Rat IFN‑β Microplate (pre‑coated)
• Rat IFN‑β Standard (800 pg/mL)
• Standard Diluent
• Rat IFN‑β Detect Antibody (biotinylated)
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

Compatible Sample Types

Serum / plasma / tissue homogenate / cell culture supernatant and other related liquid samples.

Recommended Usage

• Samples and standards are recommended to be assayed in duplicate or triplicate wells to minimize random error.
• Allow all reagents to equilibrate to room temperature for 30 minutes before the assay.
• Use disposable pipette tips to avoid cross‑contamination.
• Incubate on a low‑speed shaker or gently tap the plate every 10 minutes to ensure mixing.
• Un‑used plate strips should be promptly resealed and stored at 4 °C.

Storage & Shipping

Unopened kit: Store protected from light at 2–8 °C; shelf life 6 months.
After opening: Refer to the accompanying instruction manual for specific storage requirements of each component.
Shipping: Ship on a cold chain using ice packs (blue ice) to maintain temperature control throughout transit.

Technical Specifications

Applicable Research Areas

This kit is suitable for research projects involving dynamic changes of rat IFN‑β in viral infection, immunomodulation, inflammation models, and drug screening, enabling investigators to obtain reliable quantitative data rapidly.

Important Statement

This product is intended for research use only and must not be used for human clinical diagnosis or therapy. Users are responsible for verifying and complying with any patents or licensing requirements associated with the use of this kit; the company assumes no liability for any infringement or other legal consequences.

Note: The above information may change due to product optimization or upgrades; please contact Yakein for the most current details.