总基质金属蛋白酶9酶联免疫试剂盒(大鼠) Total MMP

总基质金属蛋白酶9 (Total MMP‑9) ELISA 试剂盒 — 大鼠专用

产品型号： KTE3041-3  |  英文名称： Rat Total MMP‑9 ELISA Kit  |  分类： 核心产品 / 细胞分析 / ELISA

产品概述

本套件基于EliKine™ 双抗体夹心ELISA技术，专为定量检测大鼠来源的全长基质金属蛋白酶9（Total MMP‑9）设计。适用于细胞培养上清、血清及血浆等样本，提供 78.13 pg/mL – 5,000 pg/mL 的线性检测范围，满足从基础研究到疾病模型的多层次需求。

关键特性 & 优势

• 极高灵敏度：检测下限 78.13 pg/mL。
• 宽检测范围：78.13 pg/mL – 5,000 pg/mL，覆盖低至中等表达水平。
• 特异性强：采用特异性抗大鼠 Total MMP‑9 抗体，未见明显交叉反应或背景干扰。
• 标准化操作：全套预包装试剂，简化实验流程。

检测原理

采用双抗体夹心酶联免疫吸附（ELISA）技术。

1. 高亲和力抗体预包被于微孔板。
2. 加入标准品、待测样本及生物素化检测抗体，形成抗原‑抗体‑抗体复合物。
3. 加入链霉亲和素‑HRP，随后加入TMB显色底物。
4. 在 450 nm（参考波长 570‑630 nm）读取吸光度，颜色深浅与 Total MMP‑9 浓度呈正比。

试剂盒组成

• Rat Total MMP‑9 Microplate
• Rat Total MMP‑9 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat Total MMP‑9 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

技术规格

检测类型	ELISA（夹心法）
反应物种	大鼠（Rat）
样本类型	细胞培养上清、血清、血浆
检测范围	78.13 pg/mL – 5,000 pg/mL
灵敏度	78.13 pg/mL

操作注意事项

• 不同批号或过期的试剂请勿混用。
• 使用前在室温放置 30 分钟使试剂平衡。
• 实验全程使用一次性吸头，防止交叉污染。
• 未使用完的板请立即放回包装、密封并置 4 ℃保存。
• 每 10 分钟轻轻摇匀或使用低速振荡器，以确保反应均匀。
• 建议对标准品和样本采用双孔或三孔复孔检测。

保存与运输

未开封存储	避光、2‑8 ℃，有效期 24 个月
开封后处理	请参照说明书对各组分分别保存
运输方式	冰袋（蓝冰）冷链运输

警示声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本试剂盒不应侵犯任何专利或许可证，若产生相关法律责任，供应商不承担责任。

背景信息

基质金属蛋白酶 9（MMP‑9）是锌依赖的金属蛋白酶，参与胚胎发育、血管生成、伤口愈合等生理过程，也与关节炎、脑出血、肿瘤转移等病理状态密切相关。监测大鼠模型中的 Total MMP‑9 水平，可帮助阐明其在免疫、心血管及神经系统疾病中的作用机制。

订购信息

规格	单价 (CNY)	货期
48 T	1,598.00	请咨询
96 T	2,558.00	期货（5 天内发货）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Total Matrix Metalloproteinase 9 (Total MMP‑9) ELISA Kit — For Rat

Catalog Number： KTE3041-3  |  English Name： Rat Total MMP‑9 ELISA Kit  |  Category： Core Products / Cell Analysis / ELISA

Product Overview

This kit is based on the EliKine™ sandwich ELISA technology and is specifically designed for the quantitative detection of full-length Matrix Metalloproteinase 9 (Total MMP‑9) from rat samples. It is suitable for samples such as cell culture supernatants, serum, and plasma, providing a linear detection range of 78.13 pg/mL – 5,000 pg/mL, meeting diverse needs from basic research to disease modeling.

Key Features & Advantages

• High Sensitivity：Lower limit of detection: 78.13 pg/mL.
• Broad Detection Range：78.13 pg/mL – 5,000 pg/mL, covering low to medium expression levels.
• High Specificity：Utilizes specific anti-rat Total MMP‑9 antibodies with no significant cross-reactivity or background interference observed.
• Standardized Procedure：Fully pre-packaged reagents simplify the experimental workflow.

Assay Principle

Utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique.

1. A high-affinity antibody is pre-coated onto the microplate.
2. Standards, test samples, and a biotinylated detection antibody are added to form an antigen-antibody-antibody complex.
3. Streptavidin-HRP is added, followed by TMB substrate for color development.
4. Absorbance is read at 450 nm (reference wavelength 570‑630 nm), with the color intensity being proportional to the Total MMP‑9 concentration.

Kit Components

• Rat Total MMP‑9 Microplate
• Rat Total MMP‑9 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat Total MMP‑9 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Technical Specifications

Assay Type	ELISA (Sandwich)
Reactivity	Rat
Sample Type	Cell Culture Supernatant, Serum, Plasma
Detection Range	78.13 pg/mL – 5,000 pg/mL
Sensitivity	78.13 pg/mL

Procedure Notes

• Do not mix reagents from different lots or expired kits.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the assay to prevent cross-contamination.
• Seal and store unused wells immediately at 4 ℃ after opening.
• Gently agitate the plate every 10 minutes or use a low-speed shaker to ensure uniform reaction.
• It is recommended to perform duplicate or triplicate measurements for standards and samples.

Storage and Shipping

Unopened Storage	Store protected from light at 2‑8 ℃; stable for 24 months.
After Opening	Please refer to the manual for individual component storage instructions.
Shipping Condition	Shipped on blue ice via cold chain.

Disclaimer

This product is for research use only and is not intended for human or clinical diagnostic use. Use of this kit should not infringe upon any patents or licenses; the supplier assumes no liability for any related legal responsibilities.

Background Information

Matrix Metalloproteinase 9 (MMP‑9) is a zinc-dependent metalloproteinase involved in physiological processes such as embryonic development, angiogenesis, and wound healing, and is also closely associated with pathological conditions like arthritis, cerebral hemorrhage, and tumor metastasis. Monitoring Total MMP‑9 levels in rat models can help elucidate its role in the mechanisms of immune, cardiovascular, and neurological diseases.

Ordering Information

Size	Unit Price (CNY)	Lead Time
48 T	1,598.00	Please Inquire
96 T	2,558.00	Future Product (Ships within 5 days)

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.