TNF-α ELISA KIT 肿瘤坏死因子-αELISA试

产品概述

英文名称：Rat TNF-α ELISA Kit 中文名称：TNF-α ELISA KIT 肿瘤坏死因子-α ELISA 试剂盒（大鼠）
货号：KTE9007-2 检测类型：ELISA（夹心法）

核心应用领域

• 蛋白类研究
• 细胞分析中的Quantitative ELISA
• 适用于细胞培养上清、血清、血浆及其他生物液体

技术背景

肿瘤坏死因子-α（TNF-α）是由活化的巨噬细胞、T 细胞、B 细胞、NK 细胞等多种细胞分泌的关键细胞因子，在宿主抗感染、肿瘤免疫及炎症反应中发挥重要作用。过量表达与败血性休克、恶病质及自身免疫紊乱密切相关。

基因ID：24835 蛋白质ID：P16599 Uniprot 详细信息

套件组成

• 大鼠源 TNF-α 抗体预包被微孔板
• 大鼠源 TNF-α 蛋白标准品
• 大鼠源 TNF-α 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

性能指标

检测范围	31.25 pg/mL – 2 000 pg/mL
灵敏度	16 pg/mL
特异性	无明显交叉反应或背景干扰（已在同类蛋白中验证）

使用建议

• 每个样品建议设置双孔或三孔复孔以提高数据可靠性。
• 实验前将试剂在室温平衡 30 分钟。
• 充分混匀是获得准确结果的关键，推荐使用低速振荡器或每 10 分钟手动摇匀一次。
• 使用一次性吸头以避免交叉污染。
• 未使用的板条请立即放回包装，密闭保存于 4 ℃。

存储与运输

• 未开启包装：2 °C ~ 8 °C 保存。
• 启用后：请按照说明书中各组分的保存要求分别保存。
• 运输方式：冰袋（蓝冰）冷链运输。

安全与合规

警告：本产品仅供科研使用，严禁用于人类或临床诊断。使用本试剂盒不构成对任何专利或许可证的侵犯。如因使用本产品导致的专利纠纷或其他合规问题，供应商概不负责。

规格与订购信息

规格	包装数量	价格（人民币）	交货期
48 T	48 个试剂盒	¥1 598.00	现货（次日发货）
96 T	96 个试剂盒	¥2 558.00	现货（次日发货）

关键检验指标

检测对象：TNF-α（大鼠）
种属：大鼠（Null 表示无特定限定）
样本类型：细胞培养上清、血清、血浆及其他生物液体

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Rat TNF-α ELISA Kit Chinese Name: TNF-α ELISA KIT Tumor Necrosis Factor‑α ELISA Kit (Rat)
Catalog No.: KTE9007-2 Assay Type: ELISA (sandwich format)

Core Application Areas

• Protein research
• Quantitative ELISA in cellular analysis
• Suitable for cell culture supernatants, serum, plasma, and other biological fluids

Technical Background

Tumor necrosis factor‑α (TNF‑α) is a key cytokine secreted by activated macrophages, T cells, B cells, NK cells and other cell types, playing crucial roles in host anti‑infection, tumor immunity, and inflammatory responses. Over‑expression is closely associated with septic shock, cachexia, and autoimmune disorders.

Gene ID: 24835 Protein ID: P16599 Uniprot detailed information

Kit Components

• Rat‑derived TNF‑α antibody‑coated microplate
• Rat‑derived TNF‑α protein standard
• Rat‑derived TNF‑α detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate sealing film

Performance Specifications

Detection Range	31.25 pg/mL – 2 000 pg/mL
Sensitivity	16 pg/mL
Specificity	No significant cross‑reactivity or background interference (validated against related proteins)

Recommended Use

• It is recommended to assign duplicate or triplicate wells for each sample to improve data reliability.
• Equilibrate reagents at room temperature for 30 minutes before the assay.
• Thorough mixing is critical for accurate results; use a low‑speed orbital shaker or manually vortex every 10 minutes.
• Use disposable pipette tips to avoid cross‑contamination.
• Unused strips should be promptly returned to their packaging and stored sealed at 4 °C.

Storage and Shipping

• Unopened: Store at 2 °C ~ 8 °C.
• After opening: Store each component according to the preservation requirements specified in the manual.
• Shipping method: Cold‑chain transport with ice packs (blue ice).

Safety and Compliance

Warning: This product is for research use only and must not be used in humans or clinical diagnostics. Use of this kit does not infringe any patents or licenses. The supplier assumes no responsibility for any patent disputes or other compliance issues arising from the use of this product.

Specifications and Ordering Information

Format	Package Quantity	Price (CNY)	Lead Time
48‑T	48 kits	¥1 598.00	In stock (next‑day shipment)
96‑T	96 kits	¥2 558.00	In stock (next‑day shipment)

Key Assay Parameters

Analyte: TNF‑α (Rat)
Species: Rat (Null indicates no specific restriction)
Sample Types: Cell culture supernatants, serum, plasma, and other biological fluids

Note: The above information may change due to product improvements or upgrades. Please consult Yakeyin Company for the most current details.