胰岛素样生长因子1酶联免疫试剂盒(小鼠) IGF-1 ELI

Mouse/Rat IGF‑1 ELISA Kit (KTE3036‑3)

中文名称：胰岛素样生长因子1酶联免疫试剂盒(小鼠) IGF‑1 ELISA KIT 产品分类：核心产品 | 细胞分析 | ELISA 检测类型：夹心法 ELISA（双抗体夹心） 适用物种：小鼠 / 大鼠

产品概述

EliKine™ 研发的 IGF‑1 夹心 ELISA 试剂盒专为小鼠和大鼠样本中的胰岛素样生长因子 1（IGF‑1）进行高灵敏度定量设计。通过预包被高亲和力抗体的微孔板、双抗体夹心结构以及 TMB 显色底物，实现对 15.63 pg/mL‑1 000 pg/mL 范围内 IGF‑1 的可靠测定。

关键特点与优势

• 极高灵敏度：检测下限 15.63 pg/mL。
• 广泛线性范围：15.63 pg/mL ~ 1 000 pg/mL，满足从细胞培养上清到血清、血浆的不同浓度需求。
• 特异性强：已验证对同类蛋白和非特异性结合的交叉反应极低。
• 操作简便：标准化试剂配方，配套 10× 检测缓冲、20× 洗涤缓冲等，降低实验误差。
• 兼容多种样本：细胞培养上清、血清、血浆均可直接使用。

检测原理

本套件采用双抗体夹心酶联免疫吸附技术：

1. 微孔板预包被特异性抗小鼠/大鼠 IGF‑1 抗体。
2. 加入标准品或待测样本，IGF‑1 与固相抗体形成捕获复合物。
3. 洗涤后加入生物素化检测抗体，实现二步特异性识别。
4. 再加入链霉亲和素‑HRP，形成酶标复合物。
5. 加入 TMB 底物，在暗处显色，随后加入终止液。
6. 在 450 nm（参考波长 570‑630 nm）测定吸光度，光密度与 IGF‑1 浓度呈正比。

试剂盒组成

每套试剂盒均含以下分装（对应 48 或 96 井板规格）：

• Mouse/Rat IGF‑1 微孔板
• Mouse/Rat IGF‑1 标准品（冻干）
• Assay Buffer (10×)
• Mouse/Rat IGF‑1 检测抗体（100×）
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

技术规格

检测指标	IGF‑1
分子	IGF‑1
检测范围	15.63 pg/mL ~ 1 000 pg/mL
灵敏度	15.63 pg/mL
样本类型	细胞培养上清、血清、血浆
反应性	小鼠 / 大鼠
试剂盒规格	48 T / 96 T
单价（人民币）	48 T  ¥1 598.00   96 T  ¥2 558.00
交货期	期货（5 天内发货）
保存条件	未开封：避光、2‑8 ℃，保质期 24 个月 开封后：请参照说明书中各组分的储存要求
运输方式	冰袋（蓝冰）冷链运输

使用注意事项

• 试剂不得与不同批号或已过期的试剂混用。
• 使用前请于室温平衡 30 分钟。
• 操作时请使用一次性吸头，防止交叉污染。
• 未用完的板条请立即回放于原包装，密封后置于 4 ℃保存。
• 混匀对结果影响显著，推荐使用低速振荡器或每 10 分钟手动轻摇。
• 建议对标准品与样品进行双孔或三孔复孔检测，以提升数据可靠性。

产品背景

IGF‑1（胰岛素样生长因子 1）是结构上类似胰岛素的多功能激素，主要由肝脏分泌并在多组织中实现旁分泌/自分泌。它是 AKT 信号通路的天然激活剂，调控细胞增殖、存活及代谢过程。IGF‑1 通过 IGF‑1 受体 (IGF1R) 与胰岛素受体介导信号传导，其水平受生长激素、营养状态及下游信号通路（如 SHP2、STAT5B）调控。异常的 IGF‑1 表达与生长障碍、代谢紊乱、神经退行性疾病、癌症以及脑血管事件密切相关。

适用研究领域

• 细胞增殖与凋亡机制研究
• 代谢与内分泌调控实验
• 药物作用机制及筛选（尤其是影响 IGF‑1 通路的化合物）
• 动物模型中 IGF‑1 动态监测
• 疾病模型（如生长迟缓、老年痴呆、肿瘤）中的生物标志物研究

免责声明

本产品仅供科研使用，不得用于人类临床诊断或治疗。使用本套件进行的任何专利或许可证相关行为均需使用者自行负责，供应商不承担因侵权或其他违规产生的责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse/Rat IGF‑1 ELISA Kit (KTE3036‑3)

Chinese Name: Insulin-like Growth Factor 1 Enzyme-Linked Immunosorbent Assay Kit (Mouse) IGF‑1 ELISA KIT Product Category: Core Products | Cell Analysis | ELISA Assay Type: Sandwich ELISA (Two-Antibody Sandwich) Species Reactivity: Mouse / Rat

Product Overview

The EliKine™ IGF‑1 Sandwich ELISA Kit is specifically designed for the highly sensitive quantification of Insulin-like Growth Factor 1 (IGF‑1) in mouse and rat samples. Utilizing a pre-coated microplate with high-affinity antibodies, a two-antibody sandwich structure, and TMB chromogenic substrate, it enables reliable determination of IGF‑1 within the range of 15.63 pg/mL to 1,000 pg/mL.

Key Features & Advantages

• High Sensitivity: Lower detection limit of 15.63 pg/mL.
• Wide Linear Range: 15.63 pg/mL to 1,000 pg/mL, meeting the concentration requirements for various samples from cell culture supernatants to serum and plasma.
• High Specificity: Validated to have minimal cross-reactivity with similar proteins and non-specific binding.
• Easy Operation: Standardized reagent formulations, including 10× Assay Buffer, 20× Wash Buffer, etc., to minimize experimental error.
• Compatible with Multiple Sample Types: Suitable for direct use with cell culture supernatants, serum, and plasma.

Assay Principle

This kit employs a two-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique:

1. The microplate is pre-coated with a specific antibody against mouse/rat IGF‑1.
2. Standards or test samples are added, allowing IGF‑1 to bind to the immobilized capture antibody.
3. After washing, a biotinylated detection antibody is added, enabling a second specific recognition step.
4. Streptavidin-HRP is then added, forming an enzyme-labeled complex.
5. TMB substrate is added, developing color in the dark, followed by the addition of a Stop Solution.
6. Absorbance is measured at 450 nm (reference wavelength 570-630 nm), with the optical density being proportional to the IGF‑1 concentration.

Kit Components

Each kit contains the following components (for 48 or 96 well plate formats):

• Mouse/Rat IGF‑1 Pre-coated Microplate
• Mouse/Rat IGF‑1 Standard (Lyophilized)
• Assay Buffer (10×)
• Mouse/Rat IGF‑1 Detection Antibody (100×)
• Streptavidin-HRP Conjugate (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Technical Specifications

Analyte	IGF‑1
Molecule	IGF‑1
Detection Range	15.63 pg/mL to 1,000 pg/mL
Sensitivity	15.63 pg/mL
Sample Types	Cell Culture Supernatant, Serum, Plasma
Reactivity	Mouse / Rat
Kit Size	48 T / 96 T
Unit Price (RMB)	48 T  ¥1,598.00   96 T  ¥2,558.00
Delivery Time	Future-dated item (Ships within 5 business days)
Storage Conditions	Unopened: Store protected from light at 2-8 ℃, Shelf life: 24 months Opened: Please refer to the storage requirements for individual components in the manual.
Shipping Method	Cold chain transport with ice packs (blue ice)

Usage Notes

• Do not mix reagents from different lot numbers or expired kits.
• Allow all reagents to reach room temperature (equilibrate for approximately 30 minutes) before use.
• Use disposable pipette tips to prevent cross-contamination.
• Return unused strips to the original foil pouch immediately, seal, and store at 4 ℃.
• Thorough mixing significantly affects results; use a low-speed plate shaker or gentle manual shaking every 10 minutes is recommended.
• It is advised to perform duplicate or triplicate measurements for standards and samples to enhance data reliability.

Product Background

IGF‑1 (Insulin-like Growth Factor 1) is a multifunctional hormone structurally similar to insulin, primarily secreted by the liver and acting in an autocrine/paracrine manner in various tissues. It is a natural activator of the AKT signaling pathway, regulating cell proliferation, survival, and metabolic processes. IGF‑1 signaling is mediated through the IGF‑1 receptor (IGF1R) and insulin receptor, with its levels regulated by growth hormone, nutritional status, and downstream signaling pathways (e.g., SHP2, STAT5B). Aberrant IGF‑1 expression is closely associated with growth disorders, metabolic diseases, neurodegenerative diseases, cancer, and cerebrovascular events.

Applicable Research Areas

• Studies on cell proliferation and apoptosis mechanisms
• Metabolic and endocrine regulation experiments
• Drug mechanism of action studies and screening (especially for compounds affecting the IGF‑1 pathway)
• Dynamic monitoring of IGF‑1 in animal models
• Biomarker research in disease models (e.g., growth retardation, Alzheimer's disease, tumors)

Disclaimer

This product is intended for research use only and is not for use in human clinical diagnosis or treatment. The user is solely responsible for any actions related to patents or licenses involving the use of this kit. The supplier assumes no liability for infringement or other violations arising from such use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.