BFGF/FGFB/FGF-2/B-FGF/HBGH-2 E

产品概述

产品名称：Rat FGF2 ELISA Kit (One Step Method) 中文名称：BFGF/FGFB/FGF-2/B‑FGF/HBGH-2 ELISA KIT 成纤维细胞生长因子ELISA试剂盒(大鼠) 货号：KTE4003-2 检测对象：大鼠成纤维细胞生长因子‑2（FGF2）

核心应用

• 蛋白类分析 – ELISA 定量检测
• 一级应用：ELISA 研究平台
• 细胞分析中心：适用于血清、血浆、组织匀浆、细胞上清及其他体外液体样本

技术特性与优势

• 极高灵敏度：检测下限 1.6 pg/mL
• 宽检测范围：8 pg/mL ~ 640 pg/mL（线性定量）
• 特异性：采用双抗体夹心法，未观察到目标蛋白类似物的交叉反应或背景干扰
• 一步法设计：样本加入后仅需两次洗涤，即可完成抗原捕获、检测抗体结合与信号放大
• 兼容性：适配市面上常见的酶标仪（450 nm）及 96/48 孔酶标板
• 操作简化：推荐使用低速振荡器或每 10 分钟手动摇匀，以确保反应均一

检测原理

本套件基于双抗体夹心ELISA：
1. 预包被抗体固定于酶标板孔壁，捕获样本中的大鼠FGF2。
2. 加入生物素化检测抗体形成抗原‑抗体复合物。
3. 链霉亲和素‑HRP结合至检测抗体，完成信号放大。
4. 加入TMB底物，在HRP催化下显色（蓝色），终止液使颜色转为黄色。
5. 使用酶标仪在 450 nm 读取 OD 值，依据标准曲线计算样本中FGF2浓度。

试剂盒组成

• Rat FGF2 Microplate（预包被）
• Rat FGF2 Standard（1,280 pg/mL）
• Standard Diluent
• Rat FGF2 Detect Antibody（生物素化）
• Streptavidin‑HRP
• HRP Substrate A & B（TMB）
• Stop Solution
• Wash Buffer
• Plate Covers

实验流程概述（建议）

1. 将试剂盒在室温（≈20‑25 °C）平衡 30 分钟。
2. 加入 50 µL 样本或标准品至对应孔中，37 °C 孵育 60 分钟。
3. 洗板 3 次（使用提供的 Wash Buffer），每次加入 300 µL 并倒掉。
4. 加入 50 µL 检测抗体，37 °C 孵育 30 分钟。
5. 重复洗涤步骤。
6. 加入 50 µL Streptavidin‑HRP，37 °C 孵育 30 分钟。
7. 再次洗涤后，加入 100 µL TMB 底物（A 与 B 合并），在暗室 15‑20 分钟显色。
8. 加入 50 µL Stop Solution，立即读 450 nm。
9. 使用标准曲线进行定量分析。

样本与检测规模

支持的样本类型包括血清、血浆、组织匀浆、细胞上清以及其他体外液体样本。为提高精确度，推荐每个样本设置双孔或三孔平行检测。

包装规格与订购信息

规格	孔数	单价 (¥)	交货周期
48 T	48 孔	1998.00	期货（3 天内发货）
96 T	96 孔	3198.00	期货（3 天内发货）

储存与运输

• 未开封：避光保存于 2‑8 °C，保质期 6 个月。
• 开封后：请参照说明书分别保存各组分（如需冷藏请保持在 4 °C，避免反复冻融）。
• 运输条件：全程使用蓝色冰袋冷链运输。

安全与合规

本产品仅限于科研用途，不适用于人类或临床诊断。使用过程中请遵守实验室生物安全规程，使用一次性吸头防止交叉污染。如因专利、许可证或其他法律限制导致的使用风险，本公司概不承担责任。

关键字检索

BFGF; FGFB; FGF-2; HBGF-2; B‑FGF; Basic Fibroblast Growth Factor; Heparin‑binding growth factor 2

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Rat FGF2 ELISA Kit (One Step Method) Chinese Name: BFGF/FGFB/FGF-2/B‑FGF/HBGH-2 ELISA KIT Fibroblast Growth Factor ELISA Kit (Rat) Item No.: KTE4003-2 Analyte: Rat Fibroblast Growth Factor‑2 (FGF2)

Core Applications

• Protein analysis – quantitative ELISA detection
• Primary application: ELISA research platform
• Cell analysis core: suitable for serum, plasma, tissue homogenates, cell supernatants, and other extracellular fluid samples

Technical Features and Advantages

• Ultra‑high sensitivity: detection limit 1.6 pg/mL
• Wide detection range: 8 pg/mL ~ 640 pg/mL (linear quantitation)
• Specificity: based on a sandwich immunoassay; no cross‑reactivity or background interference from related proteins observed
• One‑step design: after sample addition only two washes are required to complete antigen capture, detection antibody binding, and signal amplification
• Compatibility: compatible with common microplate readers (450 nm) and 96‑ or 48‑well plates
• Simplified workflow: recommended to use a low‑speed shaker or manually mix every 10 minutes to ensure uniform reaction

Assay Principle

This kit is based on a sandwich ELISA:
1. The pre‑coated capture antibody is immobilized on the microplate well surface to capture rat FGF2 from the sample.
2. Biotinylated detection antibody is added to form an antigen‑antibody complex.
3. Streptavidin‑HRP binds to the detection antibody, completing signal amplification.
4. TMB substrate is added; HRP catalyzes a color change to blue, which is stopped to yield a yellow color.
5. Optical density is read at 450 nm using a microplate reader, and the FGF2 concentration in the sample is calculated from the standard curve.

Kit Components

• Rat FGF2 Microplate (pre‑coated)
• Rat FGF2 Standard (1,280 pg/mL)
• Standard Diluent
• Rat FGF2 Detect Antibody (biotinylated)
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

Suggested Experimental Procedure Overview

1. Equilibrate the kit at room temperature (≈20‑25 °C) for 30 minutes.
2. Add 50 µL of sample or standard to the appropriate wells and incubate at 37 °C for 60 minutes.
3. Wash the plate 3 times (using the supplied Wash Buffer), adding 300 µL each time and discarding.
4. Add 50 µL detection antibody and incubate at 37 °C for 30 minutes.
5. Repeat the washing step.
6. Add 50 µL Streptavidin‑HRP and incubate at 37 °C for 30 minutes.
7. After a second wash, add 100 µL TMB substrate (A and B combined) and develop in the dark for 15‑20 minutes.
8. Add 50 µL Stop Solution and read immediately at 450 nm.
9. Quantify using the standard curve.

Sample Types and Throughput

Supported sample types include serum, plasma, tissue homogenates, cell supernatants, and other extracellular fluid samples. To improve accuracy, duplicate or triplicate wells are recommended for each sample.

Packaging Specifications and Ordering Information

Specification	Number of wells	Unit price (¥)	Lead time
48 T	48 wells	1998.00	In stock (shipped within 3 days)
96 T	96 wells	3198.00	In stock (shipped within 3 days)

Storage and Shipping

• Unopened: Store protected from light at 2‑8 °C; shelf life 6 months.
• After opening: Refer to the manual for storage of each component (refrigerate at 4 °C if needed, avoid repeated freeze‑thaw cycles).
• Shipping condition: Transport on a continuous cold chain using blue ice packs.

Safety and Compliance

The product is for research use only, not intended for human or clinical diagnostic applications. Follow laboratory biosafety procedures during use and employ disposable pipette tips to prevent cross‑contamination. The company assumes no liability for any risks arising from patents, licenses, or other legal restrictions.

Keyword Search

BFGF; FGFB; FGF-2; HBGF-2; B‑FGF; Basic Fibroblast Growth Factor; Heparin‑binding growth factor 2

Note: The above information may change due to product optimization, upgrades, or other factors; please contact Yakein Company for the latest details.