IgG4/IGHG4 ELISA KIT 免疫球蛋白G4EL

大鼠免疫球蛋白G4 (IgG4) ELISA试剂盒 (一步法)

货号: KTE4048-2

产品概述

EliKine™ 大鼠免疫球蛋白G4 (IgG4) ELISA定量试剂盒是一款基于成熟双抗体夹心法原理开发的高品质检测工具，专为大鼠血清、血浆、组织匀浆及细胞上清液等样本中IgG4的精确定量而设计。本试剂盒作为细胞分析领域的核心产品，为ELISA应用提供了可靠的技术支持，其优化的“一步法”操作流程有效缩短了实验时间，提升了实验效率。

主要特性与优势

• 高灵敏度与宽检测范围：检测下限低至20 pg/mL，线性检测范围为100 pg/mL - 8000 pg/mL，能够精准检测样本中极低浓度的目标蛋白。
• 卓越的特异性：经过严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰，确保检测结果的准确性和可靠性。
• 操作便捷：采用一步法检测流程，减少了操作步骤，有助于提高实验的可重复性并降低人为误差。
• 即用型组分：试剂盒提供预包被微孔板及即用型标准品、检测抗体等全套优化组分，开箱即用，省去繁琐的准备工作。

检测原理

本试剂盒采用双抗体夹心ELISA原理。预先包被在微孔板上的特异性抗体捕获样本中的大鼠IgG4，随后依次加入生物素化的检测抗体和链霉亲和素-HRP，形成复合物。经洗涤后加入底物TMB显色，颜色深浅与样本中大鼠IgG4的浓度成正比，通过在450 nm波长下测定OD值即可进行定量分析。

试剂盒组分

• 预包被大鼠IgG4微孔板 (Rat IgG4 Microplate)
• 大鼠IgG4标准品 (1,600 pg/mL) (Rat IgG4 Standard)
• 标准品稀释液 (Standard Diluent)
• 大鼠IgG4检测抗体 (Rat IgG4 Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A (HRP Substrate A)
• HRP底物B (HRP Substrate B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

规格与价格

规格	价格 (人民币)	货期
48 T (测试)	1,998.0	期货 (3天内发货)
96 T (测试)	3,198.0	期货 (3天内发货)

样本类型

适用于大鼠的血清、血浆、组织分浆、细胞培养上清液及相关液体样本。

保存与运输

保存建议：未开封试剂盒请避光保存于2-8℃，保质期为6个月。启用后，各组分请参照说明书建议条件保存。
运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或超出有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 实验过程中请使用一次性吸头，避免交叉污染。
• 未使用的板条应立即放回原包装袋并密封，置于4℃保存。
• 充分混匀试剂对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告： 本产品仅供科学研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。

别名信息： Immunoglobulin G4; IgG4; IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat Immunoglobulin G4 (IgG4) ELISA Kit (One-Step)

Catalog Number: KTE4048-2

Product Overview

The EliKine™ Rat Immunoglobulin G4 (IgG4) ELISA Quantitative Kit is a high-quality detection tool developed based on the mature double-antibody sandwich principle. It is specifically designed for the precise quantification of IgG4 in samples such as rat serum, plasma, tissue homogenates, and cell culture supernatants. As a core product in cell analysis, this kit provides reliable technical support for ELISA applications. Its optimized one-step operation procedure effectively reduces experimental time and improves efficiency.

Key Features & Advantages

• High Sensitivity & Wide Detection Range: The minimum detectable concentration is as low as 20 pg/mL, with a linear detection range of 100 pg/mL - 8000 pg/mL, enabling accurate detection of target proteins at very low concentrations.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with analogues of the target protein, ensuring accurate and reliable results.
• Operational Convenience: The one-step detection procedure reduces operational steps, helping to improve experimental reproducibility and minimize human error.
• Ready-to-Use Components: The kit provides pre-coated microplates and ready-to-use standards, detection antibodies, and other fully optimized components, allowing for immediate use without tedious preparation.

Detection Principle

This kit employs the double-antibody sandwich ELISA principle. A specific antibody pre-coated on the microplate captures rat IgG4 from the sample. Subsequently, a biotinylated detection antibody and Streptavidin-HRP are added sequentially to form a complex. After washing, the substrate TMB is added for color development. The color intensity is proportional to the concentration of rat IgG4 in the sample. Quantitative analysis is performed by measuring the OD value at 450 nm.

Kit Components

• Pre-coated Rat IgG4 Microplate
• Rat IgG4 Standard (1,600 pg/mL)
• Standard Diluent
• Rat IgG4 Detect Antibody
• Streptavidin-HRP
• HRP Substrate A
• HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Covers

Specifications & Price

Specification	Price (RMB)	Lead Time
48 T (Tests)	1,998.0	Stock (Ships within 3 days)
96 T (Tests)	3,198.0	Stock (Ships within 3 days)

Sample Types

Suitable for rat serum, plasma, tissue homogenates, cell culture supernatants, and related liquid samples.

Storage & Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2-8°C; the shelf life is 6 months. After opening, store components according to the conditions suggested in the manual.
Shipping Conditions: Shipped with ice packs (blue ice) to ensure reagent activity.

Important Notes

• Do not mix reagents from different lot numbers or use expired reagents.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• Use disposable pipette tips during the experiment to avoid cross-contamination.
• Unused strips should be immediately returned to the original foil pouch and sealed, stored at 4°C.
• Thorough mixing of reagents is crucial for experimental results; use a low-speed shaker or periodic manual shaking is recommended.
• For more reliable data, it is recommended to test samples and standards in replicates (duplicate or triplicate wells).

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Users should ensure their application does not violate any patents or licensing regulations.

Alias Information: Immunoglobulin G4; IgG4; IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.