JE/MCP-1/CCL2 ELISA KIT 趋化因子2E

EliKine™ 大鼠 JE/MCP-1/CCL2 趋化因子2 ELISA试剂盒 (货号: KTE3087-2)

产品概述

本产品是一款基于双抗体夹心法原理的高品质ELISA检测试剂盒，专门用于定量检测大鼠样本（包括细胞培养上清、血清、血浆）中的趋化因子配体2 (CCL2)，也称为单核细胞趋化蛋白1 (MCP-1)。该试剂盒是细胞分析领域的核心产品，具有优异的灵敏度和特异性，为炎症机制、免疫反应及神经退行性疾病等相关研究提供可靠的分析工具。

核心特性与优势

• 高灵敏度：最低检测浓度可达 7.81 pg/mL，能够精准检测低丰度表达。
• 宽广的检测范围：7.81 pg/mL - 500 pg/mL，适用于多种浓度水平的样本分析。
• 卓越的特异性：经严格验证，对目标蛋白的类似物未见明显的交叉反应或背景干扰。
• 可靠的重复性：优化的实验体系确保了结果的可重复性与准确性。
• 即用型组分：提供预包被板、标准品、检测抗体等完整组分，实验流程简化，节省准备时间。

产品规格与价格

规格	货号	价格 (人民币)	货期
48 T	KTE3087-2	1598.0	请咨询
96 T	KTE3087-2	2558.0	期货（5天内发货）

试剂盒组分

• 预包被大鼠CCL2微孔板
• 大鼠CCL2标准品（冻干）
• 检测缓冲液 (10×)
• 大鼠CCL2检测抗体 (100×)
• 链霉亲和素-HRP偶联物 (100×)
• HRP底物 (TMB)
• 终止液
• 洗涤缓冲液 (20×)
• 板盖

背景信息

趋化因子配体2 (CCL2/MCP-1) 是CC趋化因子家族的关键成员，主要由单核细胞、巨噬细胞和树突状细胞分泌。它在招募免疫细胞（如单核细胞、记忆T细胞）至炎症部位中发挥核心作用，与银屑病、类风湿性关节炎、动脉粥样硬化及多种神经退行性疾病的发病机制密切相关。

实验注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温下平衡约30分钟。
• 为避免交叉污染，建议使用一次性吸头。
• 未使用的板条应立即放回原包装袋并密封，于4℃保存。
• 实验过程中充分的混匀至关重要，推荐使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对样品和标准品进行复孔（双孔或三孔）检测。

保存与运输

保存建议：未开封的试剂盒请避光保存于2-8℃，有效期24个月。启用后，请参照说明书妥善保存各组分。
运输条件：全程采用冰袋（蓝冰）低温运输，确保试剂活性。

重要声明

本产品仅限于科学研究用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定，对于因使用本产品可能导致的任何专利侵权或其他违规行为，生产商不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Rat JE/MCP-1/CCL2 Chemokine ELISA Kit (Cat. No.: KTE3087-2)

Product Overview

This product is a high-quality ELISA detection kit based on the double-antibody sandwich principle, specifically designed for the quantitative detection of Chemokine (C-C motif) ligand 2 (CCL2), also known as Monocyte Chemoattractant Protein-1 (MCP-1), in rat samples (including cell culture supernatants, serum, and plasma). As a core product in the field of cellular analysis, this kit offers excellent sensitivity and specificity, providing a reliable analytical tool for research related to inflammatory mechanisms, immune responses, neurodegenerative diseases, and more.

Core Features & Advantages

• High Sensitivity: Minimum detectable concentration reaches 7.81 pg/mL, enabling accurate detection of low-abundance expression.
• Wide Detection Range: 7.81 pg/mL - 500 pg/mL, suitable for analyzing samples with various concentration levels.
• Excellent Specificity: Rigorously validated, showing no significant cross-reactivity or background interference with analogues of the target protein.
• Reliable Reproducibility: The optimized experimental system ensures reproducible and accurate results.
• Ready-to-Use Components: Provides complete components including pre-coated plates, standards, detection antibodies, etc., simplifying the experimental procedure and saving preparation time.

Specifications & Pricing

Specification	Catalog Number	Price (RMB)	Availability
48 T	KTE3087-2	1598.0	Please Inquire
96 T	KTE3087-2	2558.0	Future Goods (Ships within 5 days)

Kit Components

• Pre-coated Rat CCL2 Microplate
• Rat CCL2 Standard (Lyophilized)
• Detection Buffer (10×)
• Rat CCL2 Detection Antibody (100×)
• Streptavidin-HRP Conjugate (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Sealers

Background Information

Chemokine (C-C motif) ligand 2 (CCL2/MCP-1) is a key member of the CC chemokine family, primarily secreted by monocytes, macrophages, and dendritic cells. It plays a central role in recruiting immune cells (such as monocytes and memory T cells) to sites of inflammation and is closely associated with the pathogenesis of psoriasis, rheumatoid arthritis, atherosclerosis, and various neurodegenerative diseases.

Experimental Precautions

• Do not mix reagents from different lots or those that have expired.
• All reagents should be equilibrated to room temperature for approximately 30 minutes before use.
• To avoid cross-contamination, the use of disposable pipette tips is recommended.
• Unused strips should be immediately returned to the original foil pouch and sealed, stored at 4°C.
• Thorough mixing during the experiment is crucial; the use of a low-speed shaker or periodic manual shaking is recommended.
• For more reliable data, it is recommended to perform duplicate or triplicate measurements for both samples and standards.

Storage & Shipping

Storage Recommendation: Unopened kits should be stored protected from light at 2-8°C, with a shelf life of 24 months. After opening, please store components properly according to the instructions.
Shipping Conditions: Shipped under low-temperature conditions with ice packs (blue ice) throughout to ensure reagent activity.

Important Notice

This product is intended for research use only and is not for use in humans or clinical diagnosis. The user should ensure that its application does not infringe upon any patents or license regulations. The manufacturer assumes no liability for any patent infringement or other violations that may result from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.