CXCL1 ELISA KIT 趋化因子1ELISA试剂盒(

Rat CXCL1/KC ELISA Kit (KTE3038‑2)

中文名称：CXCL1 ELISA KIT 趋化因子1 ELISA试剂盒（大鼠）

产品分类：核心产品  |  产品中心：细胞分析  |  检测类型：ELISA（夹心法）

一、产品概述

本套装基于EliKine™ 双抗体夹心酶联免疫吸附技术，专为大鼠CXCL1（亦称KC、GROα、NAP‑3）在细胞培养上清、血清及血浆中的定量检测而设。高亲和力预包被板以及生物素化检测抗体确保了15.63 pg/mL的灵敏度与^{1,000 pg/mL}的线性检测范围，适用于炎症、伤口修复、血管生成及肿瘤相关研究。

二、核心技术与检测原理

• 固相抗体预包被在高亲和力酶标板孔中，实现对CXCL1的专一捕获。
• 加入标准品或待测样本后，CXCL1与固相抗体形成第一层复合物。
• 加入生物素化检测抗体（100×），形成夹心结构。
• 通过链霉亲和素‑HRP（100×）结合生物素，实现酶标记。
• 加入TMB底物显色，随CXCL1浓度呈颜色深浅，终止液后在450 nm（参考570‑630 nm）读取吸光度。

三、试剂盒组成

• Rat CXCL1 Microplate
• Rat CXCL1 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat CXCL1 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

四、主要性能指标

检测范围	15.63 pg/mL – 1,000 pg/mL
灵敏度（LOD）	15.63 pg/mL
样本类型	细胞培养上清、血清、血浆
反应物种	大鼠

五、实验步骤（建议）

1. 将酶标板、标准品、样本在室温（≈20‑25 °C）平衡30 分钟。
2. 加入100 µL标准或样本至对应孔，37 °C孵育1‑2 h（或按说明书设定时间）。
3. 洗板5次（使用Wash Buffer），每次摇晃30 秒。
4. 加入含检测抗体的混合液，37 °C再孵育1 h。
5. 重复洗板步骤后，加入Streptavidin‑HRP并孵育30 min。
6. 再次洗板，加入TMB底物，避光显色5‑15 min。
7. 加入Stop Solution终止反应，立即在450 nm读取吸光度。
8. 建议每个样本做双孔或三孔复孔，以提高数据可靠性。

六、使用注意事项

• 请勿混用不同批号或超过有效期的试剂。
• 所有操作应使用一次性吸头，防止交叉污染。
• 未使用的酶标板应立即放回包装，密封后置于4 °C保存。
• 关键步骤（如加入检测抗体、Streptavidin‑HRP）需充分混匀，推荐使用低速振荡器或每10 分钟手动摇匀。
• 样品和标准建议使用复孔检测。

七、保存与运输

未开封试剂盒：避光保存于2‑8 °C，保质期24个月。

启用后：请参照说明书对各组分分别保存。

运输方式：冰袋（蓝冰）冷链运输，以确保活性。

八、法规与声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套装不构成对任何专利或许可证的侵犯，若因使用产生法律纠纷，制造商不承担责任。

九、技术参数与订购信息

货号	KTE3038‑2
规格	48 T | 96 T
单套价格（人民币）	48 T: 1,598.0 | 96 T: 2,558.0
货期	请咨询
检测分子	CXCL1
基因/蛋白 ID	未提供

快速获取：如需进一步技术支持或样品验证方案，请联系销售代表或访问我们的官方网站。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat CXCL1/KC ELISA Kit (KTE3038‑2)

Product Name (Chinese): CXCL1 ELISA KIT Chemokine (C‑X‑C motif) Ligand 1 ELISA Reagent Kit (Rat)

Product Category: Core Product | Product Center: Cell Analysis | Detection Type: ELISA (Sandwich)

I. Product Overview

This kit utilizes EliKine™ double‑antibody sandwich ELISA technology, specifically designed for quantitative detection of rat CXCL1 (also known as KC, GROα, NAP‑3) in cell culture supernatants, serum, and plasma. The high‑affinity pre‑coated plates together with a biotinylated detection antibody provide a sensitivity of 15.63 pg/mL and a linear detection range up to ^{1,000 pg/mL}, suitable for studies on inflammation, wound healing, angiogenesis, and tumor biology.

II. Core Technology and Detection Principle

• Capture antibody is pre‑coated on high‑affinity microplate wells for specific capture of CXCL1.
• After adding standards or samples, CXCL1 binds to the solid‑phase antibody forming the first complex.
• Biotinylated detection antibody (100×) is added, creating a sandwich structure.
• Streptavidin‑HRP (100×) binds to the biotin, providing enzymatic labeling.
• TMB substrate is added for color development; the intensity correlates with CXCL1 concentration. Reaction is stopped and absorbance read at 450 nm (reference 570–630 nm).

III. Kit Components

• Rat CXCL1 Microplate
• Rat CXCL1 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat CXCL1 Detection Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

IV. Key Performance Specifications

Detection Range	15.63 pg/mL – 1,000 pg/mL
Sensitivity (LOD)	15.63 pg/mL
Sample Types	Cell culture supernatant, serum, plasma
Species Reactivity	Rat

V. Recommended Experimental Procedure

1. Equilibrate the microplate, standards, and samples at room temperature (≈20‑25 °C) for 30 minutes.
2. Add 100 µL of standard or sample to the appropriate wells and incubate at 37 °C for 1‑2 hours (or as specified in the manual).
3. Wash the plate five times with Wash Buffer, shaking 30 seconds each wash.
4. Add the detection‑antibody mix, incubate at 37 °C for an additional 1 hour.
5. Repeat the washing step, then add Streptavidin‑HRP and incubate for 30 minutes.
6. Wash again, add TMB substrate, and develop in the dark for 5‑15 minutes.
7. Add Stop Solution to terminate the reaction and immediately read absorbance at 450 nm.
8. It is recommended to run each sample in duplicate or triplicate to improve data reliability.

VI. Precautions

• Do not mix reagents from different lot numbers or use reagents past their expiration date.
• All operations should be performed with disposable tips to avoid cross‑contamination.
• Unused microplates should be returned to their original packaging, sealed, and stored at 4 °C.
• Critical steps (e.g., addition of detection antibody, Streptavidin‑HRP) must be mixed thoroughly; gentle orbital shaking or manual inversion every 10 minutes is recommended.
• Samples and standards are best analyzed using replicate wells.

VII. Storage and Shipping

Unopened kit: Store protected from light at 2‑8 °C; shelf life 24 months.

After opening: Follow the instructions to store each component individually.

Shipping method: Cold‑chain transport with ice packs (blue ice) to maintain activity.

VIII. Regulations and Disclaimer

This product is for research use only and is not intended for human or clinical diagnostic applications. Use of this kit does not infringe any patents or licenses; the manufacturer bears no liability for any legal disputes arising from its use.

IX. Technical Specifications and Ordering Information

Catalog No.	KTE3038‑2
Pack Size	48 T | 96 T
Unit Price (CNY)	48 T: 1,598.0 | 96 T: 2,558.0
Lead Time	Please inquire
Analyte	CXCL1
Gene/Protein ID	Not provided

Quick Access: For additional technical support or sample validation protocols, please contact your sales representative or visit our website.

Note: The information above may change due to product optimization or upgrades; please consult Yakein Co. for the latest details.